In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea
Abstract The refinement of neural circuits towards mature function is driven during development by patterned spontaneous calcium-dependent electrical activity. In the auditory system, this sensory-independent activity arises in the pre-hearing cochlea and regulates the survival and refinement of the...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55519-w |
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| author | Francesca De Faveri Federico Ceriani Walter Marcotti |
| author_facet | Francesca De Faveri Federico Ceriani Walter Marcotti |
| author_sort | Francesca De Faveri |
| collection | DOAJ |
| description | Abstract The refinement of neural circuits towards mature function is driven during development by patterned spontaneous calcium-dependent electrical activity. In the auditory system, this sensory-independent activity arises in the pre-hearing cochlea and regulates the survival and refinement of the auditory pathway. However, the origin and interplay of calcium signals during cochlear development is unknown in vivo. Here we show how calcium dynamics in the cochlear neuroepithelium of live pre-hearing mice shape the activity of the inner hair cells (IHCs) and their afferent synapses. Both IHCs and supporting cells (SCs) generate spontaneous calcium-dependent activity. Calcium waves from SCs synchronise the activity of nearby IHCs, which then spreads longitudinally recruiting several additional IHCs via a calcium wave-independent mechanism. This synchronised IHC activity in vivo increases the probability of afferent terminal recruitment. Moreover, the modiolar-to-pillar segregation in sound sensitivity of mature auditory nerve fibres appears to be primed at pre-hearing ages. |
| format | Article |
| id | doaj-art-8baad9ca7b2d4fcb87b5ea5fd8639806 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-8baad9ca7b2d4fcb87b5ea5fd86398062025-01-05T12:36:54ZengNature PortfolioNature Communications2041-17232025-01-0116111410.1038/s41467-024-55519-wIn vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochleaFrancesca De Faveri0Federico Ceriani1Walter Marcotti2School of Biosciences, University of SheffieldSchool of Biosciences, University of SheffieldSchool of Biosciences, University of SheffieldAbstract The refinement of neural circuits towards mature function is driven during development by patterned spontaneous calcium-dependent electrical activity. In the auditory system, this sensory-independent activity arises in the pre-hearing cochlea and regulates the survival and refinement of the auditory pathway. However, the origin and interplay of calcium signals during cochlear development is unknown in vivo. Here we show how calcium dynamics in the cochlear neuroepithelium of live pre-hearing mice shape the activity of the inner hair cells (IHCs) and their afferent synapses. Both IHCs and supporting cells (SCs) generate spontaneous calcium-dependent activity. Calcium waves from SCs synchronise the activity of nearby IHCs, which then spreads longitudinally recruiting several additional IHCs via a calcium wave-independent mechanism. This synchronised IHC activity in vivo increases the probability of afferent terminal recruitment. Moreover, the modiolar-to-pillar segregation in sound sensitivity of mature auditory nerve fibres appears to be primed at pre-hearing ages.https://doi.org/10.1038/s41467-024-55519-w |
| spellingShingle | Francesca De Faveri Federico Ceriani Walter Marcotti In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea Nature Communications |
| title | In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea |
| title_full | In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea |
| title_fullStr | In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea |
| title_full_unstemmed | In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea |
| title_short | In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea |
| title_sort | in vivo spontaneous ca2 activity in the pre hearing mammalian cochlea |
| url | https://doi.org/10.1038/s41467-024-55519-w |
| work_keys_str_mv | AT francescadefaveri invivospontaneousca2activityintheprehearingmammaliancochlea AT federicoceriani invivospontaneousca2activityintheprehearingmammaliancochlea AT waltermarcotti invivospontaneousca2activityintheprehearingmammaliancochlea |