Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions
A critical challenge in ecological restoration of open-pit mine dumps in cold regions with limited topsoil resources is how to rapidly mitigate the plant growth-inhibitory effects of mineral black clay, thereby converting it into arable soil. Leveraging the high degradation capacity of coal seam-ass...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016124005892 |
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| author | María Laura Gigena Dong Xiao Fangzhou Li Chengyu Wu Yuhong Zhang |
| author_facet | María Laura Gigena Dong Xiao Fangzhou Li Chengyu Wu Yuhong Zhang |
| author_sort | María Laura Gigena |
| collection | DOAJ |
| description | A critical challenge in ecological restoration of open-pit mine dumps in cold regions with limited topsoil resources is how to rapidly mitigate the plant growth-inhibitory effects of mineral black clay, thereby converting it into arable soil. Leveraging the high degradation capacity of coal seam-associated microorganisms on fossil carbon materials, combined with soil conditioning techniques, this study developed a microbial-based approach for modifying black clay. Seed germination experiments informed both laboratory and field trial designs. This approach focused on removing germination-inhibiting compounds, establishing a plant-compatible soil ecological environment, and employing composite strategies to reduce soil viscosity. Field experiments demonstrated that in-situ microbial modification of black clay effectively supports ecological restoration, enhances plant growth. To refine and implement this microbial-based bioremediation strategy in practical ecological restoration efforts, two key technical methods were employed: • A comprehensive experimental protocol was established for black clay bioremediation, covering both laboratory-scale and field test procedures, ensuring the approach can be readily adapted to diverse environmental conditions. • By incorporating the characteristics of local species, employing representative seed germination tests to assess plant compatibility can facilitate a rapid evaluation of the bioconversion of mineral substrates into arable soils. |
| format | Article |
| id | doaj-art-8b5d7569153e41009e9bb1cb762f70c4 |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-8b5d7569153e41009e9bb1cb762f70c42025-01-05T04:28:12ZengElsevierMethodsX2215-01612025-06-0114103138Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regionsMaría Laura Gigena0Dong Xiao1Fangzhou Li2Chengyu Wu3Yuhong Zhang4CUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology, Xuzhou, Jiangsu province, 221116, PR China; Key Laboratory of Xinjiang Coal Resources Green Mining, Ministry of Education, Xinjiang Institute of Engineering, Urumqi, 830023, PR China; CUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, Universidad Católica de Salta, Salta, A4400EDD, ArgentinaCUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology, Xuzhou, Jiangsu province, 221116, PR China; Corresponding author.CUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, Universidad Católica de Salta, Salta, A4400EDD, ArgentinaCUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology, Xuzhou, Jiangsu province, 221116, PR ChinaCUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology, Xuzhou, Jiangsu province, 221116, PR China; Key Laboratory of Xinjiang Coal Resources Green Mining, Ministry of Education, Xinjiang Institute of Engineering, Urumqi, 830023, PR ChinaA critical challenge in ecological restoration of open-pit mine dumps in cold regions with limited topsoil resources is how to rapidly mitigate the plant growth-inhibitory effects of mineral black clay, thereby converting it into arable soil. Leveraging the high degradation capacity of coal seam-associated microorganisms on fossil carbon materials, combined with soil conditioning techniques, this study developed a microbial-based approach for modifying black clay. Seed germination experiments informed both laboratory and field trial designs. This approach focused on removing germination-inhibiting compounds, establishing a plant-compatible soil ecological environment, and employing composite strategies to reduce soil viscosity. Field experiments demonstrated that in-situ microbial modification of black clay effectively supports ecological restoration, enhances plant growth. To refine and implement this microbial-based bioremediation strategy in practical ecological restoration efforts, two key technical methods were employed: • A comprehensive experimental protocol was established for black clay bioremediation, covering both laboratory-scale and field test procedures, ensuring the approach can be readily adapted to diverse environmental conditions. • By incorporating the characteristics of local species, employing representative seed germination tests to assess plant compatibility can facilitate a rapid evaluation of the bioconversion of mineral substrates into arable soils.http://www.sciencedirect.com/science/article/pii/S2215016124005892Coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions. |
| spellingShingle | María Laura Gigena Dong Xiao Fangzhou Li Chengyu Wu Yuhong Zhang Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions MethodsX Coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions. |
| title | Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions |
| title_full | Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions |
| title_fullStr | Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions |
| title_full_unstemmed | Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions |
| title_short | Methodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions |
| title_sort | methodological study on coal based microbial modification of mineral black clay to overcome plant growth challenges on open pit mine dumps in cold regions |
| topic | Coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions. |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124005892 |
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