Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis
Abstract Purpose To evaluate the advantages and clinical utility of metagenomic sequencing (MGS) in diagnosing infectious uveitis pathogens. Methods A retrospective study was conducted on 20 infectious uveitis patients (20 eyes) who received treatments at the Affiliated Eye Hospital of Shandong Univ...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-12-01
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| Series: | Journal of Ophthalmic Inflammation and Infection |
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| Online Access: | https://doi.org/10.1186/s12348-024-00449-3 |
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| author | Jinxia Yu Yane Gao Hongsheng Bi Youhua Zhang Kai Tang Dadong Guo Xiaofeng Xie |
| author_facet | Jinxia Yu Yane Gao Hongsheng Bi Youhua Zhang Kai Tang Dadong Guo Xiaofeng Xie |
| author_sort | Jinxia Yu |
| collection | DOAJ |
| description | Abstract Purpose To evaluate the advantages and clinical utility of metagenomic sequencing (MGS) in diagnosing infectious uveitis pathogens. Methods A retrospective study was conducted on 20 infectious uveitis patients (20 eyes) who received treatments at the Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine from May 2020 to February 2021. Anterior aqueous humor of the patients was collected and analyzed using MGS. Then, pathogenic microorganisms that cause uveitis were identified through bioinformatic analyses based on the sequencing data of MGS. Finally, the pathogens identified by MGS were verified using both enzyme-linked immune sorbent assay (ELISA) and quantitative PCR (qPCR). Results MGS was used to detect viral pathogens in four patients, bacterial pathogens in two patients, and viral and bacterial pathogens in one patient. Among these seven subjects, five were verified by either ELISA or qPCR. Conclusions MGS holds significant value and promising potential in diagnosing infectious uveitis pathogens. However, it cannot completely replace the traditional diagnostic techniques and still needs to be integrated with conventional methods to enhance the sensitivity and specificity of pathogen detection. As a pioneering technology, MGS will advance the field of pathogen diagnosis in infectious uveitis. |
| format | Article |
| id | doaj-art-8b3e0621ede44b15ac6a536a59f70f29 |
| institution | Kabale University |
| issn | 1869-5760 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Ophthalmic Inflammation and Infection |
| spelling | doaj-art-8b3e0621ede44b15ac6a536a59f70f292025-01-05T12:43:05ZengSpringerOpenJournal of Ophthalmic Inflammation and Infection1869-57602024-12-0114111010.1186/s12348-024-00449-3Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitisJinxia Yu0Yane Gao1Hongsheng Bi2Youhua Zhang3Kai Tang4Dadong Guo5Xiaofeng Xie6Department of Ophthalmology, Affiliated Eye Hospital of Shandong University of Traditional Chinese MedicineShandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and TherapyDepartment of Ophthalmology, Affiliated Eye Hospital of Shandong University of Traditional Chinese MedicineDepartment of Ophthalmology, Affiliated Eye Hospital of Shandong University of Traditional Chinese MedicineDepartment of Ophthalmology, Affiliated Eye Hospital of Shandong University of Traditional Chinese MedicineShandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and TherapyDepartment of Ophthalmology, Affiliated Eye Hospital of Shandong University of Traditional Chinese MedicineAbstract Purpose To evaluate the advantages and clinical utility of metagenomic sequencing (MGS) in diagnosing infectious uveitis pathogens. Methods A retrospective study was conducted on 20 infectious uveitis patients (20 eyes) who received treatments at the Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine from May 2020 to February 2021. Anterior aqueous humor of the patients was collected and analyzed using MGS. Then, pathogenic microorganisms that cause uveitis were identified through bioinformatic analyses based on the sequencing data of MGS. Finally, the pathogens identified by MGS were verified using both enzyme-linked immune sorbent assay (ELISA) and quantitative PCR (qPCR). Results MGS was used to detect viral pathogens in four patients, bacterial pathogens in two patients, and viral and bacterial pathogens in one patient. Among these seven subjects, five were verified by either ELISA or qPCR. Conclusions MGS holds significant value and promising potential in diagnosing infectious uveitis pathogens. However, it cannot completely replace the traditional diagnostic techniques and still needs to be integrated with conventional methods to enhance the sensitivity and specificity of pathogen detection. As a pioneering technology, MGS will advance the field of pathogen diagnosis in infectious uveitis.https://doi.org/10.1186/s12348-024-00449-3Metagenomic sequencingInfectious uveitisMicroorganismPathogen diagnosis |
| spellingShingle | Jinxia Yu Yane Gao Hongsheng Bi Youhua Zhang Kai Tang Dadong Guo Xiaofeng Xie Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis Journal of Ophthalmic Inflammation and Infection Metagenomic sequencing Infectious uveitis Microorganism Pathogen diagnosis |
| title | Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis |
| title_full | Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis |
| title_fullStr | Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis |
| title_full_unstemmed | Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis |
| title_short | Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis |
| title_sort | preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis |
| topic | Metagenomic sequencing Infectious uveitis Microorganism Pathogen diagnosis |
| url | https://doi.org/10.1186/s12348-024-00449-3 |
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