Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors
Several studies have demonstrated the prognostic value of circulating tumor DNA (ctDNA); however, the correlation of mean tumor molecules (MTM)/ml of plasma and mean variant allele frequency (mVAF; %) with clinical parameters is yet to be understood. In this study, we analyzed ctDNA data in a pan‐ca...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Molecular Oncology |
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| Online Access: | https://doi.org/10.1002/1878-0261.13557 |
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| author | Ekaterina Kalashnikova Vasily N. Aushev Allyson Koyen Malashevich Antony Tin Shifra Krinshpun Raheleh Salari Carly Bess Scalise Rosalyn Ram Meenakshi Malhotra Harini Ravi Himanshu Sethi Stephanie Sanchez Robert Tanner Hagelstrom Maxim Brevnov Matthew Rabinowitz Solomon Moshkevich Bernhard G. Zimmermann Minetta C. Liu Alexey Aleshin |
| author_facet | Ekaterina Kalashnikova Vasily N. Aushev Allyson Koyen Malashevich Antony Tin Shifra Krinshpun Raheleh Salari Carly Bess Scalise Rosalyn Ram Meenakshi Malhotra Harini Ravi Himanshu Sethi Stephanie Sanchez Robert Tanner Hagelstrom Maxim Brevnov Matthew Rabinowitz Solomon Moshkevich Bernhard G. Zimmermann Minetta C. Liu Alexey Aleshin |
| author_sort | Ekaterina Kalashnikova |
| collection | DOAJ |
| description | Several studies have demonstrated the prognostic value of circulating tumor DNA (ctDNA); however, the correlation of mean tumor molecules (MTM)/ml of plasma and mean variant allele frequency (mVAF; %) with clinical parameters is yet to be understood. In this study, we analyzed ctDNA data in a pan‐cancer cohort of 23 543 patients who had ctDNA testing performed using a personalized, tumor‐informed assay (Signatera™, mPCR‐NGS assay). For ctDNA‐positive patients, the correlation between MTM/ml and mVAF was examined. Two subanalyses were performed: (a) to establish the association of ctDNA with tumor volume and (b) to assess the correlation between ctDNA dynamics and patient outcomes. On a global cohort, a positive correlation between MTM/ml and mVAF was observed. Among 18 426 patients with longitudinal ctDNA measurements, 13.3% had discordant trajectories between MTM/ml and mVAF at subsequent time points. In metastatic patients receiving immunotherapy (N = 51), changes in ctDNA levels expressed both in MTM/ml and mVAF showed a statistically significant association with progression‐free survival; however, the correlation with MTM/ml was numerically stronger. |
| format | Article |
| id | doaj-art-8b2bcff23d824c9a904d5bfa7e01ecb7 |
| institution | Kabale University |
| issn | 1574-7891 1878-0261 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Oncology |
| spelling | doaj-art-8b2bcff23d824c9a904d5bfa7e01ecb72024-11-08T18:26:20ZengWileyMolecular Oncology1574-78911878-02612024-11-0118112649265710.1002/1878-0261.13557Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumorsEkaterina Kalashnikova0Vasily N. Aushev1Allyson Koyen Malashevich2Antony Tin3Shifra Krinshpun4Raheleh Salari5Carly Bess Scalise6Rosalyn Ram7Meenakshi Malhotra8Harini Ravi9Himanshu Sethi10Stephanie Sanchez11Robert Tanner Hagelstrom12Maxim Brevnov13Matthew Rabinowitz14Solomon Moshkevich15Bernhard G. Zimmermann16Minetta C. Liu17Alexey Aleshin18Natera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USANatera, Inc. Austin TX USASeveral studies have demonstrated the prognostic value of circulating tumor DNA (ctDNA); however, the correlation of mean tumor molecules (MTM)/ml of plasma and mean variant allele frequency (mVAF; %) with clinical parameters is yet to be understood. In this study, we analyzed ctDNA data in a pan‐cancer cohort of 23 543 patients who had ctDNA testing performed using a personalized, tumor‐informed assay (Signatera™, mPCR‐NGS assay). For ctDNA‐positive patients, the correlation between MTM/ml and mVAF was examined. Two subanalyses were performed: (a) to establish the association of ctDNA with tumor volume and (b) to assess the correlation between ctDNA dynamics and patient outcomes. On a global cohort, a positive correlation between MTM/ml and mVAF was observed. Among 18 426 patients with longitudinal ctDNA measurements, 13.3% had discordant trajectories between MTM/ml and mVAF at subsequent time points. In metastatic patients receiving immunotherapy (N = 51), changes in ctDNA levels expressed both in MTM/ml and mVAF showed a statistically significant association with progression‐free survival; however, the correlation with MTM/ml was numerically stronger.https://doi.org/10.1002/1878-0261.13557biomarkerscirculating tumor DNAmean tumor moleculesvariant allele frequency |
| spellingShingle | Ekaterina Kalashnikova Vasily N. Aushev Allyson Koyen Malashevich Antony Tin Shifra Krinshpun Raheleh Salari Carly Bess Scalise Rosalyn Ram Meenakshi Malhotra Harini Ravi Himanshu Sethi Stephanie Sanchez Robert Tanner Hagelstrom Maxim Brevnov Matthew Rabinowitz Solomon Moshkevich Bernhard G. Zimmermann Minetta C. Liu Alexey Aleshin Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors Molecular Oncology biomarkers circulating tumor DNA mean tumor molecules variant allele frequency |
| title | Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors |
| title_full | Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors |
| title_fullStr | Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors |
| title_full_unstemmed | Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors |
| title_short | Correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors |
| title_sort | correlation between variant allele frequency and mean tumor molecules with tumor burden in patients with solid tumors |
| topic | biomarkers circulating tumor DNA mean tumor molecules variant allele frequency |
| url | https://doi.org/10.1002/1878-0261.13557 |
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