CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excess
Abstract Background Pseudomonas aeruginosa is able to survive, grow, and cause severe infections at different sites throughout the human body owing to its ability to sense diverse signals and precisely modulate target gene expression using its abundant signaling systems. Release of zinc (Zn) and hyd...
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BMC
2024-11-01
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| Online Access: | https://doi.org/10.1186/s12866-024-03671-0 |
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| author | Ting Li Zhifeng Mo Yuying Zhao Huiluo Cao Shuo Sheng Zeling Xu |
| author_facet | Ting Li Zhifeng Mo Yuying Zhao Huiluo Cao Shuo Sheng Zeling Xu |
| author_sort | Ting Li |
| collection | DOAJ |
| description | Abstract Background Pseudomonas aeruginosa is able to survive, grow, and cause severe infections at different sites throughout the human body owing to its ability to sense diverse signals and precisely modulate target gene expression using its abundant signaling systems. Release of zinc (Zn) and hydrogen peroxide (H2O2) within the phagocyte are two major host strategies to defend against bacterial infections. It was previously shown that the response regulator CzcR controls global gene expression including catalase genes during Zn excess, but regulatory mechanisms of catalase gene expression and the role of CzcR in H2O2 tolerance remain unclear. Results In the study, comparative transcriptome analysis comprehensively described the CzcR-dependent and -independent gene regulatory pattern in P. aeruginosa during Zn excess, which revealed the counteractive co-regulation of two key H2O2-detoxifying catalase genes katA and katB through CzcR-dependent and -independent pathways in response to Zn excess. Protein-DNA interaction assay demonstrated that CzcR negatively regulates the expression of catalase genes katA and katB by directly binding to their promoters. While interestingly, we further showed that CzcR positively regulates H2O2 tolerance by inducing the catalase activity during Zn excess. Conclusion This study reported the opposite functions of CzcR in negatively regulating the expression of catalase genes katA and katB but in positively regulating the activity of catalase and H2O2 tolerance during Zn excess in P. aeruginosa. |
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| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-8b0d0c2a7d2f49b2a8652363c13ac10f2024-12-01T12:12:38ZengBMCBMC Microbiology1471-21802024-11-0124111110.1186/s12866-024-03671-0CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excessTing Li0Zhifeng Mo1Yuying Zhao2Huiluo Cao3Shuo Sheng4Zeling Xu5Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural UniversityGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural UniversityGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural UniversityDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong KongKey Laboratory of Basic Pharmacology of the Ministry of Education, Joint International Research Laboratory of Ethnomedicine of the Ministry of Education and Key Laboratory of Basic Pharmacology of Guizhou Province, Zunyi Medical UniversityGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural UniversityAbstract Background Pseudomonas aeruginosa is able to survive, grow, and cause severe infections at different sites throughout the human body owing to its ability to sense diverse signals and precisely modulate target gene expression using its abundant signaling systems. Release of zinc (Zn) and hydrogen peroxide (H2O2) within the phagocyte are two major host strategies to defend against bacterial infections. It was previously shown that the response regulator CzcR controls global gene expression including catalase genes during Zn excess, but regulatory mechanisms of catalase gene expression and the role of CzcR in H2O2 tolerance remain unclear. Results In the study, comparative transcriptome analysis comprehensively described the CzcR-dependent and -independent gene regulatory pattern in P. aeruginosa during Zn excess, which revealed the counteractive co-regulation of two key H2O2-detoxifying catalase genes katA and katB through CzcR-dependent and -independent pathways in response to Zn excess. Protein-DNA interaction assay demonstrated that CzcR negatively regulates the expression of catalase genes katA and katB by directly binding to their promoters. While interestingly, we further showed that CzcR positively regulates H2O2 tolerance by inducing the catalase activity during Zn excess. Conclusion This study reported the opposite functions of CzcR in negatively regulating the expression of catalase genes katA and katB but in positively regulating the activity of catalase and H2O2 tolerance during Zn excess in P. aeruginosa.https://doi.org/10.1186/s12866-024-03671-0P. aeruginosaTwo-component systemCzcS/CzcRCatalaseOxidative tolerance |
| spellingShingle | Ting Li Zhifeng Mo Yuying Zhao Huiluo Cao Shuo Sheng Zeling Xu CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excess BMC Microbiology P. aeruginosa Two-component system CzcS/CzcR Catalase Oxidative tolerance |
| title | CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excess |
| title_full | CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excess |
| title_fullStr | CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excess |
| title_full_unstemmed | CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excess |
| title_short | CzcR-dependent reduction of catalase gene expression and induction of catalase activity in Pseudomonas aeruginosa during zinc excess |
| title_sort | czcr dependent reduction of catalase gene expression and induction of catalase activity in pseudomonas aeruginosa during zinc excess |
| topic | P. aeruginosa Two-component system CzcS/CzcR Catalase Oxidative tolerance |
| url | https://doi.org/10.1186/s12866-024-03671-0 |
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