Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, its-1 fragment of eimeria tenella and eimeria acervulina from broiler chicken in korea
<b>Aim:</b> The objective of the present study is the simple PCR assay that permits the detection of the Eimeria tenella, Eimeria acervulina that infect domestic fowl in Korea and making phylogenetic relation with in the same and neighboring species.<p> <b>Materials and Metho...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1037 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <b>Aim:</b> The objective of the present study is the simple PCR assay
that permits the detection of the Eimeria tenella, Eimeria
acervulina that infect domestic fowl in Korea and making
phylogenetic relation with in the same and neighboring species.<p>
<b>Materials and Methods:</b> The internal transcribed spacer 1
(ITS-1) region of ribosomal RNA genes of two poultry Eimeria
species; E. tenella and E. acervulina were sequenced and
analyzed the phylogenetic relationship among them from
field isolates.<p>
<b>Results:</b> About the 273-bp and 147-bp sequence of ITS-1 of
E. tenella and E. acervulina in Korea were similar reflected
in the phylogram constructed using the partial nucleotide
sequences. In case of E. tenella for homology searching the
sequences among the same species was 98% and with E. necatrix
was 89%. In case of E. acervulina sequence similarity
within the same species was 97%, whereas the similarity E.
maxima and E. mitis were 77% and 76%, respectively. ITS-
1 region of each Eimeria species had sufficient inter-specific
sequence variation and the primer is sufficient to amplify
each target Korean isolates.<p>
<b>Conclusions:</b> The molecular method examine in the present
study appears useful for discriminating among similar Eimeria
species providing useful methods for diagnosis and epidemiology
of avian coccidial infection. |
|---|---|
| ISSN: | 1309-6958 2146-1953 |