A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets
Mycoplasma pneumonia (MPS), caused by Mycoplasma hyopneumoniae (Mhp), is a chronic, airborne respiratory disease that poses a significant threat to the global swine industry. The P97 and P46 proteins are major antigens of Mhp, with the R1 region of P97 possessing full adhesive capability. Studies ha...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1493650/full |
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| author | Yintao He Yintao He Kaiyuan Xie Kaiyuan Xie Zhongmao Yuan Zhongmao Yuan Ting Ouyang Ting Ouyang Anran Dong Anran Dong Bing Ling Bing Ling Weijun Zeng Weijun Zeng Yiqi Fang Yiqi Fang Yiwan Song Yiwan Song Lianxiang Wang Hongxing Ding Hongxing Ding Mingqiu Zhao Mingqiu Zhao Shuangqi Fan Shuangqi Fan Lin Yi Lin Yi Dongfang Zhao Dongfang Zhao Dongfang Zhao Jinding Chen Jinding Chen |
| author_facet | Yintao He Yintao He Kaiyuan Xie Kaiyuan Xie Zhongmao Yuan Zhongmao Yuan Ting Ouyang Ting Ouyang Anran Dong Anran Dong Bing Ling Bing Ling Weijun Zeng Weijun Zeng Yiqi Fang Yiqi Fang Yiwan Song Yiwan Song Lianxiang Wang Hongxing Ding Hongxing Ding Mingqiu Zhao Mingqiu Zhao Shuangqi Fan Shuangqi Fan Lin Yi Lin Yi Dongfang Zhao Dongfang Zhao Dongfang Zhao Jinding Chen Jinding Chen |
| author_sort | Yintao He |
| collection | DOAJ |
| description | Mycoplasma pneumonia (MPS), caused by Mycoplasma hyopneumoniae (Mhp), is a chronic, airborne respiratory disease that poses a significant threat to the global swine industry. The P97 and P46 proteins are major antigens of Mhp, with the R1 region of P97 possessing full adhesive capability. Studies have shown that the main antigenic regions of Mhp P42 and P65 proteins exhibit strong immunogenicity. In this study, we first linked the genes encoding P97R1 and P46 proteins to form the P97R1P65 gene and subsequently constructed three shuttle plasmids: pFBD-P97R1P46, pFBD-P97R1P46-p65, and pFBD-P65-P42. These proteins were expressed using the Bac to Bac system and formulated into subunit vaccines for mouse immunization. Mouse experiments indicated that the P97R1P46 + P65-P42 protein combination elicited higher levels of specific antibodies, IL-2, IL-4, and CD8+ T cells compared to other subunit vaccine groups, a finding further validated in subsequent mouse challenge protection experiments. Therefore, we utilized the MultiBac expression system to co-express P97R1P46, P65, and P42 proteins in the pFastMultibacDual vector for immunization experiments in piglets. The piglet immunization experiments demonstrated that the Mhp subunit vaccine prepared in this study could induce specific antibodies against Mhp, with the combination of P97R1P46, P65, and P42 proteins inducing the highest level of humoral immunity. This study provides valuable insights for the development of Mhp subunit vaccines. |
| format | Article |
| id | doaj-art-8a90dc59de5f4f8dba360a938940e092 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-8a90dc59de5f4f8dba360a938940e0922024-11-13T06:21:07ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-11-011110.3389/fvets.2024.14936501493650A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in pigletsYintao He0Yintao He1Kaiyuan Xie2Kaiyuan Xie3Zhongmao Yuan4Zhongmao Yuan5Ting Ouyang6Ting Ouyang7Anran Dong8Anran Dong9Bing Ling10Bing Ling11Weijun Zeng12Weijun Zeng13Yiqi Fang14Yiqi Fang15Yiwan Song16Yiwan Song17Lianxiang Wang18Hongxing Ding19Hongxing Ding20Mingqiu Zhao21Mingqiu Zhao22Shuangqi Fan23Shuangqi Fan24Lin Yi25Lin Yi26Dongfang Zhao27Dongfang Zhao28Dongfang Zhao29Jinding Chen30Jinding Chen31College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaWen’s Group Academy, Wen’s Foodstuffs Group Co., Ltd., Xinxing, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaWen’s Group Academy, Wen’s Foodstuffs Group Co., Ltd., Xinxing, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, ChinaMycoplasma pneumonia (MPS), caused by Mycoplasma hyopneumoniae (Mhp), is a chronic, airborne respiratory disease that poses a significant threat to the global swine industry. The P97 and P46 proteins are major antigens of Mhp, with the R1 region of P97 possessing full adhesive capability. Studies have shown that the main antigenic regions of Mhp P42 and P65 proteins exhibit strong immunogenicity. In this study, we first linked the genes encoding P97R1 and P46 proteins to form the P97R1P65 gene and subsequently constructed three shuttle plasmids: pFBD-P97R1P46, pFBD-P97R1P46-p65, and pFBD-P65-P42. These proteins were expressed using the Bac to Bac system and formulated into subunit vaccines for mouse immunization. Mouse experiments indicated that the P97R1P46 + P65-P42 protein combination elicited higher levels of specific antibodies, IL-2, IL-4, and CD8+ T cells compared to other subunit vaccine groups, a finding further validated in subsequent mouse challenge protection experiments. Therefore, we utilized the MultiBac expression system to co-express P97R1P46, P65, and P42 proteins in the pFastMultibacDual vector for immunization experiments in piglets. The piglet immunization experiments demonstrated that the Mhp subunit vaccine prepared in this study could induce specific antibodies against Mhp, with the combination of P97R1P46, P65, and P42 proteins inducing the highest level of humoral immunity. This study provides valuable insights for the development of Mhp subunit vaccines.https://www.frontiersin.org/articles/10.3389/fvets.2024.1493650/fullporcine Mycoplasma pneumoniabaculovirus expression systemsubunit vaccinesimmunityco-expression of multiple promoters |
| spellingShingle | Yintao He Yintao He Kaiyuan Xie Kaiyuan Xie Zhongmao Yuan Zhongmao Yuan Ting Ouyang Ting Ouyang Anran Dong Anran Dong Bing Ling Bing Ling Weijun Zeng Weijun Zeng Yiqi Fang Yiqi Fang Yiwan Song Yiwan Song Lianxiang Wang Hongxing Ding Hongxing Ding Mingqiu Zhao Mingqiu Zhao Shuangqi Fan Shuangqi Fan Lin Yi Lin Yi Dongfang Zhao Dongfang Zhao Dongfang Zhao Jinding Chen Jinding Chen A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets Frontiers in Veterinary Science porcine Mycoplasma pneumonia baculovirus expression system subunit vaccines immunity co-expression of multiple promoters |
| title | A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets |
| title_full | A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets |
| title_fullStr | A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets |
| title_full_unstemmed | A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets |
| title_short | A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets |
| title_sort | subunit vaccine based on p97r1 p46 p42 and p65 from mycoplasma hyopneumoniae can induce significant immune response in piglets |
| topic | porcine Mycoplasma pneumonia baculovirus expression system subunit vaccines immunity co-expression of multiple promoters |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1493650/full |
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