Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley Rats
Hyperlipidemia is a medical condition that can trigger various diseases, one of which is a neurodegenerative or neurological disease. Lactoferrin is known to have multiple protective activities, one of which is antioxidant. This study aimed to determine the potential of lactoferrin bovine to decreas...
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Universitas Sebelas Maret
2024-11-01
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| Series: | JPSCR: Journal of Pharmaceutical Science and Clinical Research |
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| Online Access: | https://jurnal.uns.ac.id/jpscr/article/view/72048 |
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| author | Havian Daulung Telium Tena Djuartina Dion Notario Linawati Hananta |
| author_facet | Havian Daulung Telium Tena Djuartina Dion Notario Linawati Hananta |
| author_sort | Havian Daulung Telium |
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| description | Hyperlipidemia is a medical condition that can trigger various diseases, one of which is a neurodegenerative or neurological disease. Lactoferrin is known to have multiple protective activities, one of which is antioxidant. This study aimed to determine the potential of lactoferrin bovine to decrease brain MDA levels in Sprague-Dawley (SD) rats induced by hyperlipidemia. This study was an in vivo experimental study using 24 male SD rats divided into six groups: normal, negative control, positive control, low-dose bovine lactoferrin (LLF), intermediate-dose bovine lactoferrin (ILF), and high-dose bovine lactoferrin (HLF). The diet in the normal group was the standard diet, and the other groups were induced by a high-fat diet (HFD). The intervention for the positive control group was simvastatin. In contrast, LLF, ILF, and HLF groups were given bovine lactoferrin doses of 100, 200, and 400 mg/kgBW, respectively. After seven weeks, all rats were necropsied, and their brains were taken to be tested for malondialdehyde (MDA) levels with an MDA assay kit using a spectrophotometer. Data was then analyzed using Shapiro-Wilk and Levene test and continued with one-way ANOVA and post-hoc Tukey test. There were significant differences (p<0.05) between the negative control group (269.99±13.50 nmol/g weight) and every bovine lactoferrin groups (219.92±22.99 nmol/g weight, 151.60±23.43 nmol/g weight, 158.16±12.33 nmol/g weight, respectively) in reducing brain MDA levels. In summary, all bovine lactoferrin groups (100, 200, and 400 mg/kgBW) significantly reduced brain MDA levels of SD rats in hyperlipidemia condition. |
| format | Article |
| id | doaj-art-8a56c1a6b542459ba1640d0c98842462 |
| institution | Kabale University |
| issn | 2503-331X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universitas Sebelas Maret |
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| series | JPSCR: Journal of Pharmaceutical Science and Clinical Research |
| spelling | doaj-art-8a56c1a6b542459ba1640d0c988424622024-12-13T07:19:31ZengUniversitas Sebelas MaretJPSCR: Journal of Pharmaceutical Science and Clinical Research2503-331X2024-11-019223224110.20961/jpscr.v9i2.7204843741Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley RatsHavian Daulung Telium0Tena Djuartina1Dion Notario2Linawati Hananta3School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, IndonesiaDepartment of Anatomy, Master Program in Biomedical Sciences, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, IndonesiaDepartment of Pharmacy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, IndonesiaDepartment of Pharmacology, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, IndonesiaHyperlipidemia is a medical condition that can trigger various diseases, one of which is a neurodegenerative or neurological disease. Lactoferrin is known to have multiple protective activities, one of which is antioxidant. This study aimed to determine the potential of lactoferrin bovine to decrease brain MDA levels in Sprague-Dawley (SD) rats induced by hyperlipidemia. This study was an in vivo experimental study using 24 male SD rats divided into six groups: normal, negative control, positive control, low-dose bovine lactoferrin (LLF), intermediate-dose bovine lactoferrin (ILF), and high-dose bovine lactoferrin (HLF). The diet in the normal group was the standard diet, and the other groups were induced by a high-fat diet (HFD). The intervention for the positive control group was simvastatin. In contrast, LLF, ILF, and HLF groups were given bovine lactoferrin doses of 100, 200, and 400 mg/kgBW, respectively. After seven weeks, all rats were necropsied, and their brains were taken to be tested for malondialdehyde (MDA) levels with an MDA assay kit using a spectrophotometer. Data was then analyzed using Shapiro-Wilk and Levene test and continued with one-way ANOVA and post-hoc Tukey test. There were significant differences (p<0.05) between the negative control group (269.99±13.50 nmol/g weight) and every bovine lactoferrin groups (219.92±22.99 nmol/g weight, 151.60±23.43 nmol/g weight, 158.16±12.33 nmol/g weight, respectively) in reducing brain MDA levels. In summary, all bovine lactoferrin groups (100, 200, and 400 mg/kgBW) significantly reduced brain MDA levels of SD rats in hyperlipidemia condition.https://jurnal.uns.ac.id/jpscr/article/view/72048bovine lactoferrinbrain mdahyperlipidemialipid peroxidation |
| spellingShingle | Havian Daulung Telium Tena Djuartina Dion Notario Linawati Hananta Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley Rats JPSCR: Journal of Pharmaceutical Science and Clinical Research bovine lactoferrin brain mda hyperlipidemia lipid peroxidation |
| title | Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley Rats |
| title_full | Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley Rats |
| title_fullStr | Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley Rats |
| title_full_unstemmed | Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley Rats |
| title_short | Therapeutic Potential of Bovine Lactoferrin to Reduce Brain Malondialdehyde Levels in Hyperlipidemia-Induced Sprague-Dawley Rats |
| title_sort | therapeutic potential of bovine lactoferrin to reduce brain malondialdehyde levels in hyperlipidemia induced sprague dawley rats |
| topic | bovine lactoferrin brain mda hyperlipidemia lipid peroxidation |
| url | https://jurnal.uns.ac.id/jpscr/article/view/72048 |
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