Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence Microscopy
Molecular expression on the vascular endothelium is critical in regulating the interaction of circulating cells with the blood vessel wall. Leukocytes as well as circulating cancer cells gain entry into tissue by interacting with adhesion molecules on the endothelial cells (EC). Molecular targets on...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00004 |
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| _version_ | 1841564550829178880 |
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| author | Judith M. Runnels Parisa Zamiri Joel A. Spencer Isreal Veilleux Xunbin Wei Alexei Bogdanov Charles P. Lin |
| author_facet | Judith M. Runnels Parisa Zamiri Joel A. Spencer Isreal Veilleux Xunbin Wei Alexei Bogdanov Charles P. Lin |
| author_sort | Judith M. Runnels |
| collection | DOAJ |
| description | Molecular expression on the vascular endothelium is critical in regulating the interaction of circulating cells with the blood vessel wall. Leukocytes as well as circulating cancer cells gain entry into tissue by interacting with adhesion molecules on the endothelial cells (EC). Molecular targets on the EC are increasingly being explored for tissue-specific delivery of therapeutic and imaging agents. Here we use in vivo immunofluorescence microscopy to visualize the endothelial molecular expression in the vasculature of live animals. High-resolution images are obtained by optical sectioning through the intact skin using in vivo confocal and multiphoton microscopy after in situ labeling of EC surface markers with fluorescent antibodies. Other vascular beds such as the bone marrow and ocular blood vessels can be imaged with little or no tissue manipulation. Live imaging is particularly useful for following the dynamic expression of inducible molecules such as E-selectin during an inflammatory response. |
| format | Article |
| id | doaj-art-8a5410a7642d4e7c853d273ee2572d64 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-8a5410a7642d4e7c853d273ee2572d642025-01-02T22:37:24ZengSAGE PublishingMolecular Imaging1536-01212006-01-01510.2310/7290.2006.0000410.2310_7290.2006.00004Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence MicroscopyJudith M. RunnelsParisa ZamiriJoel A. SpencerIsreal VeilleuxXunbin WeiAlexei BogdanovCharles P. LinMolecular expression on the vascular endothelium is critical in regulating the interaction of circulating cells with the blood vessel wall. Leukocytes as well as circulating cancer cells gain entry into tissue by interacting with adhesion molecules on the endothelial cells (EC). Molecular targets on the EC are increasingly being explored for tissue-specific delivery of therapeutic and imaging agents. Here we use in vivo immunofluorescence microscopy to visualize the endothelial molecular expression in the vasculature of live animals. High-resolution images are obtained by optical sectioning through the intact skin using in vivo confocal and multiphoton microscopy after in situ labeling of EC surface markers with fluorescent antibodies. Other vascular beds such as the bone marrow and ocular blood vessels can be imaged with little or no tissue manipulation. Live imaging is particularly useful for following the dynamic expression of inducible molecules such as E-selectin during an inflammatory response.https://doi.org/10.2310/7290.2006.00004 |
| spellingShingle | Judith M. Runnels Parisa Zamiri Joel A. Spencer Isreal Veilleux Xunbin Wei Alexei Bogdanov Charles P. Lin Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence Microscopy Molecular Imaging |
| title | Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence Microscopy |
| title_full | Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence Microscopy |
| title_fullStr | Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence Microscopy |
| title_full_unstemmed | Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence Microscopy |
| title_short | Imaging Molecular Expression on Vascular Endothelial Cells by In Vivo Immunofluorescence Microscopy |
| title_sort | imaging molecular expression on vascular endothelial cells by in vivo immunofluorescence microscopy |
| url | https://doi.org/10.2310/7290.2006.00004 |
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