Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol Nanoemulsion

In this study, thymol-loaded nanoemulsion (THYNE) was incorporated into a mixture of egg white protein and hyaluronic acid to prepare antibacterial biopolymer coatings. The oil phase of the nanoemulsion (NE) was prepared by mixing different mass ratios of thymol and corn oil. NE was formed using ult...

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Main Authors: Huajiang Zhang, Afeng Wei, Rui Chuang, Lina Xu, Cuiping Han, Hanyu Li, Ning Xia
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/13/23/3809
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author Huajiang Zhang
Afeng Wei
Rui Chuang
Lina Xu
Cuiping Han
Hanyu Li
Ning Xia
author_facet Huajiang Zhang
Afeng Wei
Rui Chuang
Lina Xu
Cuiping Han
Hanyu Li
Ning Xia
author_sort Huajiang Zhang
collection DOAJ
description In this study, thymol-loaded nanoemulsion (THYNE) was incorporated into a mixture of egg white protein and hyaluronic acid to prepare antibacterial biopolymer coatings. The oil phase of the nanoemulsion (NE) was prepared by mixing different mass ratios of thymol and corn oil. NE was formed using ultrasonic emulsification, and the physicochemical properties of the NE were investigated. When the content of thymol in the oil phase was 30%, the particle size reached a minimum of 107.93 nm, PDI was 0.167, and Zeta potential was −18.2 mV, and it remained kinetically stable after 4 weeks of storage at 4 °C. Based on this study, composite coatings containing 5%, 10% and 20% THYNE were prepared, and the rheological properties, microstructure, FTIR, release properties and antibacterial properties of the coatings were investigated. The results show that the coating solutions exhibited shear thinning behavior. With increasing THYNE content, the coating structure became loose and inhomogeneous. The release rate of THY in the coatings was greater in 95% ethanol–water solution than in deionized water. In addition, the coating solutions showed stronger antibacterial activity against <i>Staphylococcus aureus</i> than against <i>Escherichia coli</i>. The egg white protein-based composite coating containing THYNE developed in this study is expected to be an antibacterial material for food packaging with sustained release performance.
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id doaj-art-8a3e313c35cf4c7293ff76f574796b1f
institution Kabale University
issn 2304-8158
language English
publishDate 2024-11-01
publisher MDPI AG
record_format Article
series Foods
spelling doaj-art-8a3e313c35cf4c7293ff76f574796b1f2024-12-13T16:26:40ZengMDPI AGFoods2304-81582024-11-011323380910.3390/foods13233809Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol NanoemulsionHuajiang Zhang0Afeng Wei1Rui Chuang2Lina Xu3Cuiping Han4Hanyu Li5Ning Xia6College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaIn this study, thymol-loaded nanoemulsion (THYNE) was incorporated into a mixture of egg white protein and hyaluronic acid to prepare antibacterial biopolymer coatings. The oil phase of the nanoemulsion (NE) was prepared by mixing different mass ratios of thymol and corn oil. NE was formed using ultrasonic emulsification, and the physicochemical properties of the NE were investigated. When the content of thymol in the oil phase was 30%, the particle size reached a minimum of 107.93 nm, PDI was 0.167, and Zeta potential was −18.2 mV, and it remained kinetically stable after 4 weeks of storage at 4 °C. Based on this study, composite coatings containing 5%, 10% and 20% THYNE were prepared, and the rheological properties, microstructure, FTIR, release properties and antibacterial properties of the coatings were investigated. The results show that the coating solutions exhibited shear thinning behavior. With increasing THYNE content, the coating structure became loose and inhomogeneous. The release rate of THY in the coatings was greater in 95% ethanol–water solution than in deionized water. In addition, the coating solutions showed stronger antibacterial activity against <i>Staphylococcus aureus</i> than against <i>Escherichia coli</i>. The egg white protein-based composite coating containing THYNE developed in this study is expected to be an antibacterial material for food packaging with sustained release performance.https://www.mdpi.com/2304-8158/13/23/3809thymol nanoemulsionegg white proteinedible coatingantibacterial packaging
spellingShingle Huajiang Zhang
Afeng Wei
Rui Chuang
Lina Xu
Cuiping Han
Hanyu Li
Ning Xia
Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol Nanoemulsion
Foods
thymol nanoemulsion
egg white protein
edible coating
antibacterial packaging
title Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol Nanoemulsion
title_full Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol Nanoemulsion
title_fullStr Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol Nanoemulsion
title_full_unstemmed Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol Nanoemulsion
title_short Preparation and Characterization of Egg White Protein-Based Composite Edible Coating Containing Thymol Nanoemulsion
title_sort preparation and characterization of egg white protein based composite edible coating containing thymol nanoemulsion
topic thymol nanoemulsion
egg white protein
edible coating
antibacterial packaging
url https://www.mdpi.com/2304-8158/13/23/3809
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