INVESTIGATION OF THE EFFECTS OF CHLORPYRIFOS AND TANNIC ACID APPLIED TO HEK 293 CELLS IN DIFFERENT DOSES AND TIMES ON CELL VITALITY
Objective: Chlorpyrifos, an organophosphate pesticide, is a widely used insecticide and shows its effect with poisoning by inhibiting the activity of acetylcholinesterase, causing acetylcholine accumulation in neuromuscular junctions, heart muscle, smooth muscle and central nervous system. Pesticide...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2023-02-01
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| Series: | Sabiad |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/F82A3654CB5642259A76810D592AD11F |
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| Summary: | Objective: Chlorpyrifos, an organophosphate pesticide, is a widely used insecticide and shows its effect with poisoning by inhibiting the activity of acetylcholinesterase, causing acetylcholine accumulation in neuromuscular junctions, heart muscle, smooth muscle and central nervous system. Pesticides are risk factors for cancer, neurodegenerative diseases, neurotoxicity, reproductive dysfunction and deterioration of many organ functions. Tannic acid is a polyphenol used in traditional and modern medicine with its many properties such as antioxidant, anti-inflammatory and anticarcinogenic. Our study was planned to investigate the possible effects of the application of chlorpyrifos and tannic acid at different doses and durations on the cell viability of the human embryonic kidney cell HEK293 cell line. Materials and Method: The study was carried out on 3 groups as control group, chlorpyrifos group and tannic acid group. MTT and xCELLigence analysis methods were used to determine at which doses and at what time chlorpyrifos and tannic acid were more effective on cell viability in HEK 293 cells. For this purpose, incubation was carried out by adding 10, 30, 60 and 90 μg/ml chlorpyrifos and 5, 10, 15, 20 and 25 μM tannic acid to the cell culture medium. Results: It was observed that the cell viability of the 30 μg/ml, 60 μg/ml and 90 μg/ml chlorpyrifos groups decreased significantly over the 24, 48 and 72 hours compared to the control group. The IC50 value of chlorpyrifos was found to be 79.94 μg/ml at 24-hour exposure. It was determined that the absorbance value of the group in which 25 μM of tannic acid was applied decreased significantly compared to the other study groups (p<0.001). Conclusion: It has been observed that chlorpyrifos is effective on cell viability especially at high doses and decreases viability more. It has been determined that the effective dose of tannic acid in increasing cell viability is 10µM and it is effective in reducing viability at high doses. |
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| ISSN: | 2651-4060 |