Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitro
Escherichia coli (E. coli) is a gram-negative bacterium extremely relevant in the context of public health, being involved in the development of several diseases worldwide. The main treatment available for infections caused by E. coli is the use of antibiotics. However, several strains of this bact...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2024-12-01
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| Series: | Acta Scientiarum: Biological Sciences |
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| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/67572 |
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| _version_ | 1846126433050558464 |
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| author | Igor Pereira Ribeiro Muniz Stefano Pedro de Melo Calado Israel Souza Ribeiro Maria Poliana Leite Galantini Caroline Vieira Gonçalves Luciano Pereira Rosa Francine Cristina Silva Rosa Robson Amaro Augusto da Silva |
| author_facet | Igor Pereira Ribeiro Muniz Stefano Pedro de Melo Calado Israel Souza Ribeiro Maria Poliana Leite Galantini Caroline Vieira Gonçalves Luciano Pereira Rosa Francine Cristina Silva Rosa Robson Amaro Augusto da Silva |
| author_sort | Igor Pereira Ribeiro Muniz |
| collection | DOAJ |
| description |
Escherichia coli (E. coli) is a gram-negative bacterium extremely relevant in the context of public health, being involved in the development of several diseases worldwide. The main treatment available for infections caused by E. coli is the use of antibiotics. However, several strains of this bacterium have shown resistance to available antibiotics, necessitating the development of alternative treatment methodologies. In this scenario, Antimicrobial Photodynamic Therapy (aPDT) emerges as an excellent viable alternative to antibiotic use. This study aimed to standardize protocols based on aPDT utilizing curcumin as photosensitizer activated by blue LED light for the control of E. coli in vitro. Curcumin solutions of 60, 80, and 100 μg mL-1 were made and photoactivated by blue LED light with fluencies of 6.6, 13.2, and 19.8 J cm-2. E. coli (ATCC® 25922 ™) was cultured for 24 hours at 37 °C. Bacterial suspensions of 1.5 x 106 CFU mL-1 were exposed to aPDT treatments. Control groups were established for each light dose and curcumin concentration. Protocols using 80 and 100 μg mL-1 curcumin solution with19.8 J cm-2 irradiation effectively inhibited bacterial growth, suggesting significant antibacterial activity (p-value < 0.05), and are recommended for this purpose.
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| format | Article |
| id | doaj-art-8a131297f64a4e50b7958cba5ee2a824 |
| institution | Kabale University |
| issn | 1679-9283 1807-863X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj-art-8a131297f64a4e50b7958cba5ee2a8242024-12-12T18:55:16ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2024-12-0146110.4025/actascibiolsci.v46i1.67572Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitroIgor Pereira Ribeiro Muniz0Stefano Pedro de Melo Calado1Israel Souza Ribeiro2Maria Poliana Leite Galantini3Caroline Vieira Gonçalves4Luciano Pereira Rosa5Francine Cristina Silva Rosa6Robson Amaro Augusto da Silva7Universidade Federal da Bahia / Universidade Estadual do Sudoeste da BahiaUniversidade Federal da BahiaUniversidade Federal da Bahia / Universidade Federal do Sul da BahiaUniversidade Federal da BahiaUniversidade Federal da BahiaUniversidade Federal da BahiaUniversidade Federal da BahiaUniversidade Federal da Bahia Escherichia coli (E. coli) is a gram-negative bacterium extremely relevant in the context of public health, being involved in the development of several diseases worldwide. The main treatment available for infections caused by E. coli is the use of antibiotics. However, several strains of this bacterium have shown resistance to available antibiotics, necessitating the development of alternative treatment methodologies. In this scenario, Antimicrobial Photodynamic Therapy (aPDT) emerges as an excellent viable alternative to antibiotic use. This study aimed to standardize protocols based on aPDT utilizing curcumin as photosensitizer activated by blue LED light for the control of E. coli in vitro. Curcumin solutions of 60, 80, and 100 μg mL-1 were made and photoactivated by blue LED light with fluencies of 6.6, 13.2, and 19.8 J cm-2. E. coli (ATCC® 25922 ™) was cultured for 24 hours at 37 °C. Bacterial suspensions of 1.5 x 106 CFU mL-1 were exposed to aPDT treatments. Control groups were established for each light dose and curcumin concentration. Protocols using 80 and 100 μg mL-1 curcumin solution with19.8 J cm-2 irradiation effectively inhibited bacterial growth, suggesting significant antibacterial activity (p-value < 0.05), and are recommended for this purpose. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/67572microbiology; photochemotherapy; optics and photonics. |
| spellingShingle | Igor Pereira Ribeiro Muniz Stefano Pedro de Melo Calado Israel Souza Ribeiro Maria Poliana Leite Galantini Caroline Vieira Gonçalves Luciano Pereira Rosa Francine Cristina Silva Rosa Robson Amaro Augusto da Silva Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitro Acta Scientiarum: Biological Sciences microbiology; photochemotherapy; optics and photonics. |
| title | Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitro |
| title_full | Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitro |
| title_fullStr | Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitro |
| title_full_unstemmed | Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitro |
| title_short | Evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of Escherichia coli in vitro |
| title_sort | evaluation of different protocols using antimicrobial photodynamic therapy with curcumin and blue led light control of escherichia coli in vitro |
| topic | microbiology; photochemotherapy; optics and photonics. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/67572 |
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