MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa
Abstract MotY homologs are present in a variety of monotrichous bacterial strains and are thought to form an additional structural T ring in flagellar motors. While MotY potentially plays an important role in motor torque generation, its impact on motor output dynamics remains poorly understood. In...
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BMC
2024-11-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-024-03602-z |
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| author | Sanyuan Fu Maojin Tian Min Chen Zhengyu Wu Rongjing Zhang Junhua Yuan |
| author_facet | Sanyuan Fu Maojin Tian Min Chen Zhengyu Wu Rongjing Zhang Junhua Yuan |
| author_sort | Sanyuan Fu |
| collection | DOAJ |
| description | Abstract MotY homologs are present in a variety of monotrichous bacterial strains and are thought to form an additional structural T ring in flagellar motors. While MotY potentially plays an important role in motor torque generation, its impact on motor output dynamics remains poorly understood. In this study, we investigate the role of MotY in P. aeruginosa, elucidating its interactions with the two sets of stator units (MotAB and MotCD) using Förster resonance energy transfer (FRET) assays. Employing a newly developed bead assay, we characterize the dynamic behavior of flagellar motors in motY mutants, identifying MotY as the key functional protein to affect the clockwise bias of naturally unbiased motors in P. aeruginosa. Our findings reveal that MotY enhances stator assembly efficiency without affecting the overall assembly of the flagellar structure. Additionally, we demonstrate that MotY is essential for maintaining motor torque and regulating switching rates. Our study highlights the physiological significance of MotY in fine-tuning flagellar motor function in complex environments. |
| format | Article |
| id | doaj-art-8a0acd27f4e246818d1c08bf06b7169d |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-8a0acd27f4e246818d1c08bf06b7169d2024-11-10T12:10:55ZengBMCBMC Microbiology1471-21802024-11-0124111110.1186/s12866-024-03602-zMotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosaSanyuan Fu0Maojin Tian1Min Chen2Zhengyu Wu3Rongjing Zhang4Junhua Yuan5Hefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of ChinaCenter of Translational Medicine, Zibo Central Hospital Affiliated to Binzhou Medical UniversityHefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of ChinaResearch Center of Translational Medicine, Central Hospital Affiliated to Shandong First Medical UniversityHefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of ChinaHefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of ChinaAbstract MotY homologs are present in a variety of monotrichous bacterial strains and are thought to form an additional structural T ring in flagellar motors. While MotY potentially plays an important role in motor torque generation, its impact on motor output dynamics remains poorly understood. In this study, we investigate the role of MotY in P. aeruginosa, elucidating its interactions with the two sets of stator units (MotAB and MotCD) using Förster resonance energy transfer (FRET) assays. Employing a newly developed bead assay, we characterize the dynamic behavior of flagellar motors in motY mutants, identifying MotY as the key functional protein to affect the clockwise bias of naturally unbiased motors in P. aeruginosa. Our findings reveal that MotY enhances stator assembly efficiency without affecting the overall assembly of the flagellar structure. Additionally, we demonstrate that MotY is essential for maintaining motor torque and regulating switching rates. Our study highlights the physiological significance of MotY in fine-tuning flagellar motor function in complex environments.https://doi.org/10.1186/s12866-024-03602-zStatorFRETBead assayFlagellumBacterial motility |
| spellingShingle | Sanyuan Fu Maojin Tian Min Chen Zhengyu Wu Rongjing Zhang Junhua Yuan MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa BMC Microbiology Stator FRET Bead assay Flagellum Bacterial motility |
| title | MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa |
| title_full | MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa |
| title_fullStr | MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa |
| title_full_unstemmed | MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa |
| title_short | MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa |
| title_sort | moty modulates proton driven flagellar motor output in pseudomonas aeruginosa |
| topic | Stator FRET Bead assay Flagellum Bacterial motility |
| url | https://doi.org/10.1186/s12866-024-03602-z |
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