Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia
Several members of the epidermal growth factor (EGF) family have been implicated in the biology of schizophrenia (Ketharanathan et al., 2024). The EGF-related ligand, Betacellulin (BTC), plays an important role in the proliferation and differentiation of neural stem cells and our group found markedl...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-10-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506125001588 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849227017267970048 |
|---|---|
| author | Agustin Cota-Coronado Murray Manning Dong-Hyung Kim Joohyung Lee Andrew Gibbons Joseph Rosenbluh Rachel A. Hill Suresh Sundram |
| author_facet | Agustin Cota-Coronado Murray Manning Dong-Hyung Kim Joohyung Lee Andrew Gibbons Joseph Rosenbluh Rachel A. Hill Suresh Sundram |
| author_sort | Agustin Cota-Coronado |
| collection | DOAJ |
| description | Several members of the epidermal growth factor (EGF) family have been implicated in the biology of schizophrenia (Ketharanathan et al., 2024). The EGF-related ligand, Betacellulin (BTC), plays an important role in the proliferation and differentiation of neural stem cells and our group found markedly reduced BTC levels in patients with schizophrenia. Nevertheless, the interplay of affected BTC and its participation in neural specification and neurodevelopment remains elusive. We generated Knockout (KO) − BTC clones from an existing hiPSC line through CRISPR/Cas9-mediated modification. Furthermore, we validated BTC-KO through genotyping/sequencing, FACS and Western Blot. Finally, we demonstrated trilineage differentiation potential in vitro. |
| format | Article |
| id | doaj-art-89a8090c30a04f22a45464f76d17151c |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-89a8090c30a04f22a45464f76d17151c2025-08-24T05:12:00ZengElsevierStem Cell Research1873-50612025-10-018810380810.1016/j.scr.2025.103808Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophreniaAgustin Cota-Coronado0Murray Manning1Dong-Hyung Kim2Joohyung Lee3Andrew Gibbons4Joseph Rosenbluh5Rachel A. Hill6Suresh Sundram7Department of Psychiatry, School of Clinical Sciences, Monash University, Clayton, Victoria 3168, Australia; Corresponding author.Cancer Research Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia; Functional Genomics Platform, Monash University, Clayton, VIC, AustraliaDepartment of Psychiatry, School of Clinical Sciences, Monash University, Clayton, Victoria 3168, AustraliaDepartment of Psychiatry, School of Clinical Sciences, Monash University, Clayton, Victoria 3168, Australia; Centre for Endocrinology and Metabolism, Hudson Institute of Medical Research, Clayton, Victoria 3168, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3168, AustraliaDepartment of Psychiatry, School of Clinical Sciences, Monash University, Clayton, Victoria 3168, AustraliaCancer Research Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia; Functional Genomics Platform, Monash University, Clayton, VIC, AustraliaDepartment of Psychiatry, School of Clinical Sciences, Monash University, Clayton, Victoria 3168, AustraliaDepartment of Psychiatry, School of Clinical Sciences, Monash University, Clayton, Victoria 3168, Australia; Mental Health Program, Monash Health, Clayton, Victoria 3168, AustraliaSeveral members of the epidermal growth factor (EGF) family have been implicated in the biology of schizophrenia (Ketharanathan et al., 2024). The EGF-related ligand, Betacellulin (BTC), plays an important role in the proliferation and differentiation of neural stem cells and our group found markedly reduced BTC levels in patients with schizophrenia. Nevertheless, the interplay of affected BTC and its participation in neural specification and neurodevelopment remains elusive. We generated Knockout (KO) − BTC clones from an existing hiPSC line through CRISPR/Cas9-mediated modification. Furthermore, we validated BTC-KO through genotyping/sequencing, FACS and Western Blot. Finally, we demonstrated trilineage differentiation potential in vitro.http://www.sciencedirect.com/science/article/pii/S1873506125001588 |
| spellingShingle | Agustin Cota-Coronado Murray Manning Dong-Hyung Kim Joohyung Lee Andrew Gibbons Joseph Rosenbluh Rachel A. Hill Suresh Sundram Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia Stem Cell Research |
| title | Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia |
| title_full | Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia |
| title_fullStr | Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia |
| title_full_unstemmed | Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia |
| title_short | Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia |
| title_sort | generation of two betacellulin crispr cas9 knockout hipsc lines to study the affected egf system paradigm in schizophrenia |
| url | http://www.sciencedirect.com/science/article/pii/S1873506125001588 |
| work_keys_str_mv | AT agustincotacoronado generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia AT murraymanning generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia AT donghyungkim generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia AT joohyunglee generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia AT andrewgibbons generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia AT josephrosenbluh generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia AT rachelahill generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia AT sureshsundram generationoftwobetacellulincrisprcas9knockouthipsclinestostudytheaffectedegfsystemparadigminschizophrenia |