Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome
Background This research investigates the metabolic profiles of follicular fluid (FF) samples from patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilisation and aims to identify diagnostic and therapeutic biomarkers for PCOS through lipidomic analysis.Methods We performed non-...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Journal of Obstetrics and Gynaecology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2378489 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841553160162770944 |
|---|---|
| author | Qing He Xiaoli Guo Wenqiang Lv Junchao Cui Jing Meng Xiao Gao Jiachen Ma Nan Zhou Yijuan Cao |
| author_facet | Qing He Xiaoli Guo Wenqiang Lv Junchao Cui Jing Meng Xiao Gao Jiachen Ma Nan Zhou Yijuan Cao |
| author_sort | Qing He |
| collection | DOAJ |
| description | Background This research investigates the metabolic profiles of follicular fluid (FF) samples from patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilisation and aims to identify diagnostic and therapeutic biomarkers for PCOS through lipidomic analysis.Methods We performed non-targeted lipid analysis of FF samples from women with PCOS (n = 6) and normal controls (n = 6) using ultra-high-performance liquid chromatography–tandem mass spectrometry. Differential lipids between the two groups were screened using multidimensional statistical analysis, followed by fold change analysis and t-tests to identify potential PCOS biomarkers.Results Multivariate statistical analysis revealed significant differences in FF lipid levels between the PCOS and control groups. Five different lipids were selected as standards, with p < .05. Phosphatidylcholine (PC), the main differentially expressed lipid, was significantly increased in the FF of the POCS group and was closely related to other lipids.Conclusions Using ultra-high-performance liquid chromatography–tandem mass spectrometry, we investigated lipid biomarkers based on FF lipidomics to provide useful information for the discovery of diagnostic markers for PCOS. Our study identified five distinct lipids as potential markers of PCOS, with PC being the primary aberrant lipid found in the FF of patients with PCOS. |
| format | Article |
| id | doaj-art-8994e79839174e808722d82a80d81862 |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-8994e79839174e808722d82a80d818622025-01-09T12:13:17ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2378489Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndromeQing He0Xiaoli Guo1Wenqiang Lv2Junchao Cui3Jing Meng4Xiao Gao5Jiachen Ma6Nan Zhou7Yijuan Cao8Affiliated Xuzhou Clinical College of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, ChinaCollege of Pharmacy, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, ChinaCollege of Pharmacy, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, ChinaCollege of Pharmacy, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, ChinaCollege of Pharmacy, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, ChinaCollege of Pharmacy, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, ChinaCollege of Pharmacy, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, ChinaCollege of Pharmacy, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, ChinaAffiliated Xuzhou Clinical College of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, ChinaBackground This research investigates the metabolic profiles of follicular fluid (FF) samples from patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilisation and aims to identify diagnostic and therapeutic biomarkers for PCOS through lipidomic analysis.Methods We performed non-targeted lipid analysis of FF samples from women with PCOS (n = 6) and normal controls (n = 6) using ultra-high-performance liquid chromatography–tandem mass spectrometry. Differential lipids between the two groups were screened using multidimensional statistical analysis, followed by fold change analysis and t-tests to identify potential PCOS biomarkers.Results Multivariate statistical analysis revealed significant differences in FF lipid levels between the PCOS and control groups. Five different lipids were selected as standards, with p < .05. Phosphatidylcholine (PC), the main differentially expressed lipid, was significantly increased in the FF of the POCS group and was closely related to other lipids.Conclusions Using ultra-high-performance liquid chromatography–tandem mass spectrometry, we investigated lipid biomarkers based on FF lipidomics to provide useful information for the discovery of diagnostic markers for PCOS. Our study identified five distinct lipids as potential markers of PCOS, with PC being the primary aberrant lipid found in the FF of patients with PCOS.https://www.tandfonline.com/doi/10.1080/01443615.2024.2378489Polycystic ovary syndromelipidomicsbiomarkersfollicular fluidultra-high-performance liquid chromatography–tandem mass spectrometry |
| spellingShingle | Qing He Xiaoli Guo Wenqiang Lv Junchao Cui Jing Meng Xiao Gao Jiachen Ma Nan Zhou Yijuan Cao Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome Journal of Obstetrics and Gynaecology Polycystic ovary syndrome lipidomics biomarkers follicular fluid ultra-high-performance liquid chromatography–tandem mass spectrometry |
| title | Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome |
| title_full | Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome |
| title_fullStr | Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome |
| title_full_unstemmed | Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome |
| title_short | Follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome |
| title_sort | follicular fluid lipidomics analysis reveals altered lipid signatures in patients with polycystic ovary syndrome |
| topic | Polycystic ovary syndrome lipidomics biomarkers follicular fluid ultra-high-performance liquid chromatography–tandem mass spectrometry |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2378489 |
| work_keys_str_mv | AT qinghe follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT xiaoliguo follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT wenqianglv follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT junchaocui follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT jingmeng follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT xiaogao follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT jiachenma follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT nanzhou follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome AT yijuancao follicularfluidlipidomicsanalysisrevealsalteredlipidsignaturesinpatientswithpolycysticovarysyndrome |