Identification of poultry meat using random amplified polymorphic dna (rapd) technique
In this study, meats of 8 poultry species (chicken, turkey, gull, ostrich, duck, goose, quail, partridge) ware identified by random amplified polymorphic DNA (RAPD) method using two different primers of 10 nucleotides each (primer 1- CAA TCG CCG T, primer 2- CCA CAG CAG T). A 45-cyclus PCR (each cyc...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=147 |
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| Summary: | In this study, meats of 8 poultry species (chicken, turkey, gull, ostrich, duck, goose, quail, partridge) ware identified by random amplified polymorphic DNA (RAPD) method using two different primers of 10 nucleotides each (primer 1- CAA TCG CCG T, primer 2- CCA CAG CAG T). A 45-cyclus PCR (each cycle; 45 s at 94°C, 45 s at 364C, and 60 s at 72°C) was performed for identification of poultry flesh. Results concluded that RAPD is a practical, rapid, and reliable method that can be used for identification of poultry meals in control of adulteration of consumers from exposure to the flesh thai the society normally does not consume. |
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| ISSN: | 1309-6958 2146-1953 |