Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings

Abstract Background Adventitious root (AR) formation is the key step for successful cutting propagation of tea plants (Camellia sinensis L.). Studies showed that arbuscular mycorrhizal fungus (AMF) can promote the rooting ability, and auxin pathway in basal stem of cuttings was involved in this proc...

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Main Authors: Weili Chen, Tingting Niu, Wenxu Lian, Tao Ye, Qinyu Sun, Jiaxia Zhang
Format: Article
Language:English
Published: BMC 2024-12-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-024-05955-z
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author Weili Chen
Tingting Niu
Wenxu Lian
Tao Ye
Qinyu Sun
Jiaxia Zhang
author_facet Weili Chen
Tingting Niu
Wenxu Lian
Tao Ye
Qinyu Sun
Jiaxia Zhang
author_sort Weili Chen
collection DOAJ
description Abstract Background Adventitious root (AR) formation is the key step for successful cutting propagation of tea plants (Camellia sinensis L.). Studies showed that arbuscular mycorrhizal fungus (AMF) can promote the rooting ability, and auxin pathway in basal stem of cuttings was involved in this process. However, auxin and abscisic acid (another important regulator on AR formation) in the other parts of cuttings at different rooting stages responding to AMF inoculation are not well studied. Therefore, in this paper, contents, enzymes and genes related to these two plant hormones were comprehensively determined aiming to unveil how endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA) involve in the AMF regulating AR development of tea cuttings. Results Inoculating with AMF significantly increased the proportion of cuttings at S2 stage (AR formation), which was more than twice as much as the control. And the total rooting rate in mycorrhizal treatment was also higher than that in the control with an increase of 8.66%. Enzyme activity assays showed that except for decreased polyphenol oxidase (PPO) activity at the S3 stage and peroxidase (POD) activity in middle stem of S3 stage, AMF inoculation increased activities of POD, PPO, superoxide dismutase (SOD) and catalase (CAT) to varying degrees in leaf, middle stem and basal stem of tea cuttings. After inoculation with AMF, the indoleacetic acid oxidase (IAAO) activity decreased to a certain extent in the first three stages of tea cuttings, which showed a trend of ‘low-high-low’ in the basal stem of all treatments. Besides, there was a significantly positive correlation between SOD activity and AR formation, especially for the proportion of cuttings at S2 and S3 stages. Higher IAA level and IAA/ABA ratio was found in basal stem of cuttings at S1 stage induced by AMF, which promoting the AR formation as revealed by correlation analysis. At the same time, AMF significantly elevated the level of IAA in leaf at S1 stage. By screening differentially expressed genes (DEGs) related to IAA and ABA pathways, together with redundant analysis, it was indicated that auxin biosynthesis and transport, as well as ABA transport and signal transduction, were involved in AMF regulating the rooting of tea cuttings. Conclusions Overall, both endogenous IAA and ABA played roles in the regulation of AR formation of tea cuttings by AMF inoculating, enriching the theoretical basis of AMF regulating rooting of cuttings and providing foundations for cutting propagation of tea plants.
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spelling doaj-art-89512a25d4d14b149dd10f8ea6b2f54a2024-12-29T12:15:15ZengBMCBMC Plant Biology1471-22292024-12-0124111810.1186/s12870-024-05955-zInvolvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttingsWeili Chen0Tingting Niu1Wenxu Lian2Tao Ye3Qinyu Sun4Jiaxia Zhang5Tea Research Institute, Anhui Academy of Agricultural SciencesTea Research Institute, Anhui Academy of Agricultural SciencesTea Research Institute, Anhui Academy of Agricultural SciencesTea Research Institute, Anhui Academy of Agricultural SciencesTea Research Institute, Anhui Academy of Agricultural SciencesTea Research Institute, Anhui Academy of Agricultural SciencesAbstract Background Adventitious root (AR) formation is the key step for successful cutting propagation of tea plants (Camellia sinensis L.). Studies showed that arbuscular mycorrhizal fungus (AMF) can promote the rooting ability, and auxin pathway in basal stem of cuttings was involved in this process. However, auxin and abscisic acid (another important regulator on AR formation) in the other parts of cuttings at different rooting stages responding to AMF inoculation are not well studied. Therefore, in this paper, contents, enzymes and genes related to these two plant hormones were comprehensively determined aiming to unveil how endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA) involve in the AMF regulating AR development of tea cuttings. Results Inoculating with AMF significantly increased the proportion of cuttings at S2 stage (AR formation), which was more than twice as much as the control. And the total rooting rate in mycorrhizal treatment was also higher than that in the control with an increase of 8.66%. Enzyme activity assays showed that except for decreased polyphenol oxidase (PPO) activity at the S3 stage and peroxidase (POD) activity in middle stem of S3 stage, AMF inoculation increased activities of POD, PPO, superoxide dismutase (SOD) and catalase (CAT) to varying degrees in leaf, middle stem and basal stem of tea cuttings. After inoculation with AMF, the indoleacetic acid oxidase (IAAO) activity decreased to a certain extent in the first three stages of tea cuttings, which showed a trend of ‘low-high-low’ in the basal stem of all treatments. Besides, there was a significantly positive correlation between SOD activity and AR formation, especially for the proportion of cuttings at S2 and S3 stages. Higher IAA level and IAA/ABA ratio was found in basal stem of cuttings at S1 stage induced by AMF, which promoting the AR formation as revealed by correlation analysis. At the same time, AMF significantly elevated the level of IAA in leaf at S1 stage. By screening differentially expressed genes (DEGs) related to IAA and ABA pathways, together with redundant analysis, it was indicated that auxin biosynthesis and transport, as well as ABA transport and signal transduction, were involved in AMF regulating the rooting of tea cuttings. Conclusions Overall, both endogenous IAA and ABA played roles in the regulation of AR formation of tea cuttings by AMF inoculating, enriching the theoretical basis of AMF regulating rooting of cuttings and providing foundations for cutting propagation of tea plants.https://doi.org/10.1186/s12870-024-05955-zArbuscular mycorrhizal fungusTea plantAdventitious rootAuxinAbscisic acidCutting propagation
spellingShingle Weili Chen
Tingting Niu
Wenxu Lian
Tao Ye
Qinyu Sun
Jiaxia Zhang
Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings
BMC Plant Biology
Arbuscular mycorrhizal fungus
Tea plant
Adventitious root
Auxin
Abscisic acid
Cutting propagation
title Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings
title_full Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings
title_fullStr Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings
title_full_unstemmed Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings
title_short Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings
title_sort involvement of endogenous iaa and aba in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant camellia sinensis l cuttings
topic Arbuscular mycorrhizal fungus
Tea plant
Adventitious root
Auxin
Abscisic acid
Cutting propagation
url https://doi.org/10.1186/s12870-024-05955-z
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