Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro
Ischemic heart disease is the main cause of death in western countries and its burden is increasing worldwide. It typically involves irreversible degeneration and loss of myocardial tissue leading to poor prognosis and fatal outcome. Autologous cells with the potential to regenerate damaged heart ti...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/247091 |
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| author | Klaus Neef Philipp Treskes Guoxing Xu Florian Drey Sureshkumar Perumal Srinivasan Tomo Saric Erastus Nembo Judith Semmler Filomain Nguemo Christof Stamm Douglas B. Cowan Antje-Christin Deppe Maximilian Scherner Thorsten Wittwer Jürgen Hescheler Thorsten Wahlers Yeong-Hoon Choi |
| author_facet | Klaus Neef Philipp Treskes Guoxing Xu Florian Drey Sureshkumar Perumal Srinivasan Tomo Saric Erastus Nembo Judith Semmler Filomain Nguemo Christof Stamm Douglas B. Cowan Antje-Christin Deppe Maximilian Scherner Thorsten Wittwer Jürgen Hescheler Thorsten Wahlers Yeong-Hoon Choi |
| author_sort | Klaus Neef |
| collection | DOAJ |
| description | Ischemic heart disease is the main cause of death in western countries and its burden is increasing worldwide. It typically involves irreversible degeneration and loss of myocardial tissue leading to poor prognosis and fatal outcome. Autologous cells with the potential to regenerate damaged heart tissue would be an ideal source for cell therapeutic approaches. Here, we compared different methods of conditional culture for increasing the yield and cardiogenic potential of murine skeletal muscle-derived stem cells. A subpopulation of nonadherent cells was isolated from skeletal muscle by preplating and applying cell culture conditions differing in support of cluster formation. In contrast to static culture conditions, dynamic culture with or without previous hanging drop preculture led to significantly increased cluster diameters and the expression of cardiac specific markers on the protein and mRNA level. Whole-cell patch-clamp studies revealed similarities to pacemaker action potentials and responsiveness to cardiac specific pharmacological stimuli. This data indicates that skeletal muscle-derived stem cells are capable of adopting enhanced cardiac muscle cell-like properties by applying specific culture conditions. Choosing this route for the establishment of a sustainable, autologous source of cells for cardiac therapies holds the potential of being clinically more acceptable than transgenic manipulation of cells. |
| format | Article |
| id | doaj-art-88fdd522903a4e5cbbd25eb6b942b251 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-88fdd522903a4e5cbbd25eb6b942b2512025-02-03T05:47:16ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/247091247091Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In VitroKlaus Neef0Philipp Treskes1Guoxing Xu2Florian Drey3Sureshkumar Perumal Srinivasan4Tomo Saric5Erastus Nembo6Judith Semmler7Filomain Nguemo8Christof Stamm9Douglas B. Cowan10Antje-Christin Deppe11Maximilian Scherner12Thorsten Wittwer13Jürgen Hescheler14Thorsten Wahlers15Yeong-Hoon Choi16Department of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyInstitute for Neurophysiology, University of Cologne, 50931 Cologne, GermanyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyInstitute for Neurophysiology, University of Cologne, 50931 Cologne, GermanyInstitute for Neurophysiology, University of Cologne, 50931 Cologne, GermanyInstitute for Neurophysiology, University of Cologne, 50931 Cologne, GermanyInstitute for Neurophysiology, University of Cologne, 50931 Cologne, GermanyBerlin-Brandenburg Center for Regenerative Therapies, 13353 Berlin, GermanyDepartment of Anesthesiology, Perioperative and Pain Medicine, Children’s Hospital Boston and Harvard Medical School, Boston, MA 02115, USADepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyInstitute for Neurophysiology, University of Cologne, 50931 Cologne, GermanyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, GermanyIschemic heart disease is the main cause of death in western countries and its burden is increasing worldwide. It typically involves irreversible degeneration and loss of myocardial tissue leading to poor prognosis and fatal outcome. Autologous cells with the potential to regenerate damaged heart tissue would be an ideal source for cell therapeutic approaches. Here, we compared different methods of conditional culture for increasing the yield and cardiogenic potential of murine skeletal muscle-derived stem cells. A subpopulation of nonadherent cells was isolated from skeletal muscle by preplating and applying cell culture conditions differing in support of cluster formation. In contrast to static culture conditions, dynamic culture with or without previous hanging drop preculture led to significantly increased cluster diameters and the expression of cardiac specific markers on the protein and mRNA level. Whole-cell patch-clamp studies revealed similarities to pacemaker action potentials and responsiveness to cardiac specific pharmacological stimuli. This data indicates that skeletal muscle-derived stem cells are capable of adopting enhanced cardiac muscle cell-like properties by applying specific culture conditions. Choosing this route for the establishment of a sustainable, autologous source of cells for cardiac therapies holds the potential of being clinically more acceptable than transgenic manipulation of cells.http://dx.doi.org/10.1155/2015/247091 |
| spellingShingle | Klaus Neef Philipp Treskes Guoxing Xu Florian Drey Sureshkumar Perumal Srinivasan Tomo Saric Erastus Nembo Judith Semmler Filomain Nguemo Christof Stamm Douglas B. Cowan Antje-Christin Deppe Maximilian Scherner Thorsten Wittwer Jürgen Hescheler Thorsten Wahlers Yeong-Hoon Choi Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro Stem Cells International |
| title | Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro |
| title_full | Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro |
| title_fullStr | Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro |
| title_full_unstemmed | Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro |
| title_short | Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro |
| title_sort | dynamic support culture of murine skeletal muscle derived stem cells improves their cardiogenic potential in vitro |
| url | http://dx.doi.org/10.1155/2015/247091 |
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