Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcoding
IntroductionSalvia miltiorrhiza radix et rhizoma (Danshen) is a crucial medicinal material for treating cardiovascular and cerebrovascular diseases. However, the presence of adulterants and intraspecific variability poses challenges to its clinical safety.MethodsThis study collected samples of S. mi...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1518906/full |
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| author | Gaojie He Jinhui Man Ying Chen Xiaoqin Zhang Xin Wang Kelu An Laha Amu Wenqin Chen Baowei Wang Yue Shi Yue Shi Xiaohui Wang Xiaohui Wang Shengli Wei Shengli Wei |
| author_facet | Gaojie He Jinhui Man Ying Chen Xiaoqin Zhang Xin Wang Kelu An Laha Amu Wenqin Chen Baowei Wang Yue Shi Yue Shi Xiaohui Wang Xiaohui Wang Shengli Wei Shengli Wei |
| author_sort | Gaojie He |
| collection | DOAJ |
| description | IntroductionSalvia miltiorrhiza radix et rhizoma (Danshen) is a crucial medicinal material for treating cardiovascular and cerebrovascular diseases. However, the presence of adulterants and intraspecific variability poses challenges to its clinical safety.MethodsThis study collected samples of S. miltiorrhiza from various regions and commonly encountered adulterants. The composition differences of S. miltiorrhiza radix and its adulterants were analyzed by fingerprint and broad-target metabolomics. Chloroplast genome was used to distinguish intra-genus species and DNA barcoding was used to identify germplasm sources.ResultsThe fingerprinting analysis proved that there is no chemical composition consistency between S. miltiorrhiza radix and its adulterants. Broad-targeted metabolomics can distinguish S. miltiorrhiza radix from Salvia yunnanensis radix, Dipsacus asperoides radix, and Arctium lappa radix. Additionally, comparative chloroplast genome analysis indicated that atpF and rps4-trnT-UGU were the potential DNA barcodes for S. miltiorrhiza. 259 samples from 13 provinces and 21 origins were amplified and sequenced, resulting in the identification of 62 haplotypes. The unique haplotypes found in Shanxi Luoyang, Shandong Qingdao and other places can be used as molecular geographic markers for the identification of the germplasm source of S. miltiorrhiza.DiscussionThis study systematically differentiates S. miltiorrhiza from its adulterants and highlights the potential of unique haplotypes as markers for sourcing. The findings provide strong scientific evidence for the clinical safety of S. miltiorrhiza, emphasizing the importance of proper cultivation, selection, and breeding of varieties. |
| format | Article |
| id | doaj-art-88cbeb17000f4f9da1e2a9b079bc2496 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-88cbeb17000f4f9da1e2a9b079bc24962025-01-07T06:40:41ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15189061518906Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcodingGaojie He0Jinhui Man1Ying Chen2Xiaoqin Zhang3Xin Wang4Kelu An5Laha Amu6Wenqin Chen7Baowei Wang8Yue Shi9Yue Shi10Xiaohui Wang11Xiaohui Wang12Shengli Wei13Shengli Wei14School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaEngineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing, ChinaSchool of Biology and Medicine, Beijing City University, Beijing, ChinaEngineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing, ChinaModern Research Center for Traditional Chinese Medicine, Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaEngineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing, ChinaIntroductionSalvia miltiorrhiza radix et rhizoma (Danshen) is a crucial medicinal material for treating cardiovascular and cerebrovascular diseases. However, the presence of adulterants and intraspecific variability poses challenges to its clinical safety.MethodsThis study collected samples of S. miltiorrhiza from various regions and commonly encountered adulterants. The composition differences of S. miltiorrhiza radix and its adulterants were analyzed by fingerprint and broad-target metabolomics. Chloroplast genome was used to distinguish intra-genus species and DNA barcoding was used to identify germplasm sources.ResultsThe fingerprinting analysis proved that there is no chemical composition consistency between S. miltiorrhiza radix and its adulterants. Broad-targeted metabolomics can distinguish S. miltiorrhiza radix from Salvia yunnanensis radix, Dipsacus asperoides radix, and Arctium lappa radix. Additionally, comparative chloroplast genome analysis indicated that atpF and rps4-trnT-UGU were the potential DNA barcodes for S. miltiorrhiza. 259 samples from 13 provinces and 21 origins were amplified and sequenced, resulting in the identification of 62 haplotypes. The unique haplotypes found in Shanxi Luoyang, Shandong Qingdao and other places can be used as molecular geographic markers for the identification of the germplasm source of S. miltiorrhiza.DiscussionThis study systematically differentiates S. miltiorrhiza from its adulterants and highlights the potential of unique haplotypes as markers for sourcing. The findings provide strong scientific evidence for the clinical safety of S. miltiorrhiza, emphasizing the importance of proper cultivation, selection, and breeding of varieties.https://www.frontiersin.org/articles/10.3389/fphar.2024.1518906/fullSalvia miltiorrhizatargeted metabolomicschloroplast genomeDNA barcodinggermplasm resource identification |
| spellingShingle | Gaojie He Jinhui Man Ying Chen Xiaoqin Zhang Xin Wang Kelu An Laha Amu Wenqin Chen Baowei Wang Yue Shi Yue Shi Xiaohui Wang Xiaohui Wang Shengli Wei Shengli Wei Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcoding Frontiers in Pharmacology Salvia miltiorrhiza targeted metabolomics chloroplast genome DNA barcoding germplasm resource identification |
| title | Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcoding |
| title_full | Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcoding |
| title_fullStr | Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcoding |
| title_full_unstemmed | Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcoding |
| title_short | Identification of Salvia miltiorrhiza germplasm resources based on metabolomics and DNA barcoding |
| title_sort | identification of salvia miltiorrhiza germplasm resources based on metabolomics and dna barcoding |
| topic | Salvia miltiorrhiza targeted metabolomics chloroplast genome DNA barcoding germplasm resource identification |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1518906/full |
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