Analysis of immune-related microRNAs in Bovine milk exosomes and construction of their regulatory networks

Bovine colostrum is rich in many immune components. Milk exosomes are secreted by mammary epithelial cells and contain proteins, nucleic acids, microRNAs (miRNAs) and other bioactive substances related to immune response and growth. MiRNAs are short non-coding RNAs (ncRNA)with various biological fun...

Full description

Saved in:
Bibliographic Details
Main Authors: Lele Wang, Gaofeng Wang, Meng Zhao, Rugang Tian
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Applied Animal Research
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/09712119.2025.2512724
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bovine colostrum is rich in many immune components. Milk exosomes are secreted by mammary epithelial cells and contain proteins, nucleic acids, microRNAs (miRNAs) and other bioactive substances related to immune response and growth. MiRNAs are short non-coding RNAs (ncRNA)with various biological functions through translational inhibition or mRNA destabilisation. Mature miRNAs are involved in various regulatory pathways, including development, viral defense, cell proliferation and apoptosis, and fat metabolism. MiRNA sequencing of bovine colostrum and milk exosomes was performed, and the miRNA and mRNA data were jointly analyzed. The results suggest that bta-miR-19b, bta-miR-186 and bta-miR-151-5p may be key miRNAs associated with the immune function of bovine colostrum exosomes. Target gene prediction revealed that miRNAs are involved in multiple signalling pathways through target genes such as IL1R1, BOLA-DOA, MAP3K1, MAPK8, RELA and TNF. Our results indicate that specific miRNAs and their target genes likely contribute to immune regulation, yet the exact molecular mechanisms governing these interactions remain to be fully elucidated.
ISSN:0971-2119
0974-1844