CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expression
Abstract Circular RNA (circRNA), a stable ring‐shaped RNA molecule found in eukaryotic cells, plays significant roles in biological regulation, particularly by interfering with transcription factor binding or enhancing gene expression. Using transcriptomic sequencing, we identified differentially ex...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Animal Research and One Health |
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| Online Access: | https://doi.org/10.1002/aro2.76 |
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| _version_ | 1846166554255818752 |
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| author | Peng Yang Xinmiao Li Lei Du Shijie Lyu Zijing Zhang Fengpeng Lin Xinglei Qi Xian Liu Eryao Wang Chuzhao Lei Yongzhen Huang |
| author_facet | Peng Yang Xinmiao Li Lei Du Shijie Lyu Zijing Zhang Fengpeng Lin Xinglei Qi Xian Liu Eryao Wang Chuzhao Lei Yongzhen Huang |
| author_sort | Peng Yang |
| collection | DOAJ |
| description | Abstract Circular RNA (circRNA), a stable ring‐shaped RNA molecule found in eukaryotic cells, plays significant roles in biological regulation, particularly by interfering with transcription factor binding or enhancing gene expression. Using transcriptomic sequencing, we identified differentially expressed circRNAs in bovine muscle at various time points. Specifically, circDYRK1A was discovered and shown to enhance differentiation while suppressing proliferation of adult myoblasts. Rescue experiments further demonstrated that circDYRK1A regulates the KLF5 gene expression by interacting with miR21‐5p, thus exerting its influence at the transcriptional level. This study marks the first identification of circDYRK1A in cattle and elucidates its role in bovine myoblast development through the circDYRK1A‐miR21‐5p‐KLF5 regulatory axis. These findings contribute novel insights into molecular breeding of cattle and advance fundamental research on beef cattle breeding and muscle development. |
| format | Article |
| id | doaj-art-881549f166894c209875e9d62b6e39a0 |
| institution | Kabale University |
| issn | 2835-5075 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Research and One Health |
| spelling | doaj-art-881549f166894c209875e9d62b6e39a02024-11-15T14:19:40ZengWileyAnimal Research and One Health2835-50752024-11-012443144510.1002/aro2.76CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expressionPeng Yang0Xinmiao Li1Lei Du2Shijie Lyu3Zijing Zhang4Fengpeng Lin5Xinglei Qi6Xian Liu7Eryao Wang8Chuzhao Lei9Yongzhen Huang10Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi Province College of Animal Science and Technology Northwest A&F University Yangling Shaanxi ChinaKey Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi Province College of Animal Science and Technology Northwest A&F University Yangling Shaanxi ChinaKey Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi Province College of Animal Science and Technology Northwest A&F University Yangling Shaanxi ChinaInstitute of Animal Husbandry and Veterinary Science Henan Academy of Agricultural Sciences Zhengzhou Henan ChinaInstitute of Animal Husbandry and Veterinary Science Henan Academy of Agricultural Sciences Zhengzhou Henan ChinaBureau of Animal Husbandry of Biyang County Biyang ChinaBureau of Animal Husbandry of Biyang County Biyang ChinaHenan Provincial Animal Husbandry General Station Zhengzhou Henan ChinaInstitute of Animal Husbandry and Veterinary Science Henan Academy of Agricultural Sciences Zhengzhou Henan ChinaKey Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi Province College of Animal Science and Technology Northwest A&F University Yangling Shaanxi ChinaKey Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi Province College of Animal Science and Technology Northwest A&F University Yangling Shaanxi ChinaAbstract Circular RNA (circRNA), a stable ring‐shaped RNA molecule found in eukaryotic cells, plays significant roles in biological regulation, particularly by interfering with transcription factor binding or enhancing gene expression. Using transcriptomic sequencing, we identified differentially expressed circRNAs in bovine muscle at various time points. Specifically, circDYRK1A was discovered and shown to enhance differentiation while suppressing proliferation of adult myoblasts. Rescue experiments further demonstrated that circDYRK1A regulates the KLF5 gene expression by interacting with miR21‐5p, thus exerting its influence at the transcriptional level. This study marks the first identification of circDYRK1A in cattle and elucidates its role in bovine myoblast development through the circDYRK1A‐miR21‐5p‐KLF5 regulatory axis. These findings contribute novel insights into molecular breeding of cattle and advance fundamental research on beef cattle breeding and muscle development.https://doi.org/10.1002/aro2.76bovinecircRNAKLF5miR21‐5pskeletal muscle |
| spellingShingle | Peng Yang Xinmiao Li Lei Du Shijie Lyu Zijing Zhang Fengpeng Lin Xinglei Qi Xian Liu Eryao Wang Chuzhao Lei Yongzhen Huang CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expression Animal Research and One Health bovine circRNA KLF5 miR21‐5p skeletal muscle |
| title | CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expression |
| title_full | CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expression |
| title_fullStr | CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expression |
| title_full_unstemmed | CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expression |
| title_short | CircDYRK1A regulates bovine myoblasts development by binding miR21‐5p to affect KLF5 gene expression |
| title_sort | circdyrk1a regulates bovine myoblasts development by binding mir21 5p to affect klf5 gene expression |
| topic | bovine circRNA KLF5 miR21‐5p skeletal muscle |
| url | https://doi.org/10.1002/aro2.76 |
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