Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testing
Abstract Aneurysm rupture is a life-threatening event, yet its underlying mechanisms remain largely unclear. This study investigated the fracture properties of the thoracic aneurysmatic aorta (TAA) using the symmetry-constraint Compact Tension (symconCT) test and compared results to native and enzym...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83233-6 |
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| author | Marta Alloisio Antti Siika David Freiholtz Anders Franco-Cereceda Joy Roy Hanna M. Björck T. Christian Gasser |
| author_facet | Marta Alloisio Antti Siika David Freiholtz Anders Franco-Cereceda Joy Roy Hanna M. Björck T. Christian Gasser |
| author_sort | Marta Alloisio |
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| description | Abstract Aneurysm rupture is a life-threatening event, yet its underlying mechanisms remain largely unclear. This study investigated the fracture properties of the thoracic aneurysmatic aorta (TAA) using the symmetry-constraint Compact Tension (symconCT) test and compared results to native and enzymatic-treated porcine aortas’ tests. With age, the aortic stiffness increased, and tissues ruptured at lower fracture energy $$D$$ . Patients with bicuspid aortic valves were more sensitive to age, had stronger aortas and required more $$D$$ than tricuspid valves individuals (peak load: axial loading 4.42 $$\pm$$ 1.56 N vs 2.51 $$\pm$$ 1.60 N; circumferential loading 5.76 $$\pm$$ 2.43 N vs 4.82 $$\pm$$ 1.49 N. Fracture energy: axial loading 1.92 $$\pm$$ 0.60 kJ m-2 vs 0.74 $$\pm$$ 0.50 kJ m-2; circumferential loading 2.12 $$\pm$$ 2.39 kJ m-2 vs 1.47 $$\pm$$ 0.91 kJ m-2). Collagen content partly explained the variability in $$D$$ , especially in bicuspid cases. Besides the primary crack, TAAs and enzymatic-treated porcine aortas displayed diffuse and shear-dominated dissection and tearing. As human tissue tests resembled enzymatic-treated porcine aortas, microstructural degeneration, including elastin loss and collagen degeneration, seems to be the main cause of TAA wall weakening. Additionally, a tortuous crack developing during the symconCT test reflected intact fracture toughening mechanisms and might characterize a healthier aorta. |
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| institution | Kabale University |
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| spelling | doaj-art-8813ace5bb0944ca83316635a288a0df2025-01-05T12:18:52ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-83233-6Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testingMarta Alloisio0Antti Siika1David Freiholtz2Anders Franco-Cereceda3Joy Roy4Hanna M. Björck5T. Christian Gasser6Department of Engineering Mechanics, KTH Royal Institute of TechnologyDepartment of Molecular Medicine and Surgery, Karolinska InstitutetSection of Cardiothoracic Surgery, Department of Molecular Medicine and Surgery, Karolinska InstitutetSection of Cardiothoracic Surgery, Department of Molecular Medicine and Surgery, Karolinska InstitutetDepartment of Molecular Medicine and Surgery, Karolinska InstitutetDivision of Cardiology, Center for Molecular Medicine, Department of Medicine, Karolinska University Hospital, Karolinska Institutet, SolnaDepartment of Engineering Mechanics, KTH Royal Institute of TechnologyAbstract Aneurysm rupture is a life-threatening event, yet its underlying mechanisms remain largely unclear. This study investigated the fracture properties of the thoracic aneurysmatic aorta (TAA) using the symmetry-constraint Compact Tension (symconCT) test and compared results to native and enzymatic-treated porcine aortas’ tests. With age, the aortic stiffness increased, and tissues ruptured at lower fracture energy $$D$$ . Patients with bicuspid aortic valves were more sensitive to age, had stronger aortas and required more $$D$$ than tricuspid valves individuals (peak load: axial loading 4.42 $$\pm$$ 1.56 N vs 2.51 $$\pm$$ 1.60 N; circumferential loading 5.76 $$\pm$$ 2.43 N vs 4.82 $$\pm$$ 1.49 N. Fracture energy: axial loading 1.92 $$\pm$$ 0.60 kJ m-2 vs 0.74 $$\pm$$ 0.50 kJ m-2; circumferential loading 2.12 $$\pm$$ 2.39 kJ m-2 vs 1.47 $$\pm$$ 0.91 kJ m-2). Collagen content partly explained the variability in $$D$$ , especially in bicuspid cases. Besides the primary crack, TAAs and enzymatic-treated porcine aortas displayed diffuse and shear-dominated dissection and tearing. As human tissue tests resembled enzymatic-treated porcine aortas, microstructural degeneration, including elastin loss and collagen degeneration, seems to be the main cause of TAA wall weakening. Additionally, a tortuous crack developing during the symconCT test reflected intact fracture toughening mechanisms and might characterize a healthier aorta.https://doi.org/10.1038/s41598-024-83233-6 |
| spellingShingle | Marta Alloisio Antti Siika David Freiholtz Anders Franco-Cereceda Joy Roy Hanna M. Björck T. Christian Gasser Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testing Scientific Reports |
| title | Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testing |
| title_full | Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testing |
| title_fullStr | Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testing |
| title_full_unstemmed | Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testing |
| title_short | Fracture properties of porcine versus human thoracic aortas from tricuspid/bicuspid aortic valve patients via symmetry-constraint Compact Tension testing |
| title_sort | fracture properties of porcine versus human thoracic aortas from tricuspid bicuspid aortic valve patients via symmetry constraint compact tension testing |
| url | https://doi.org/10.1038/s41598-024-83233-6 |
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