Research on the Interaction between Eight Polyphenols and Arachin
This study investigated the interaction between arachin and eight common polyphenols—ferulic acid, resveratrol, hesperidin, catechin, quercetin, curcumin, gallic acid, and myricetin. The interaction patterns of these polyphenols with arachin were analyzed using UV-visible and fluorescence spectrosco...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2025-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020208 |
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| author | Zuwei ZHANG Rurui LI Cong YANG Yongyan MENG Mingjuan SHEN Jian SUN Xuechun ZHANG |
| author_facet | Zuwei ZHANG Rurui LI Cong YANG Yongyan MENG Mingjuan SHEN Jian SUN Xuechun ZHANG |
| author_sort | Zuwei ZHANG |
| collection | DOAJ |
| description | This study investigated the interaction between arachin and eight common polyphenols—ferulic acid, resveratrol, hesperidin, catechin, quercetin, curcumin, gallic acid, and myricetin. The interaction patterns of these polyphenols with arachin were analyzed using UV-visible and fluorescence spectroscopy, with a focus on their effects on protein structure, surface hydrophobicity and solubility. UV-visible spectroscopy revealed that the polyphenols altered the microenvironment surrounding the amino acid residues of arachin, leading to changes in its molecular conformation. Fluorescence spectroscopy identified static quenching as the quenching mechanism of polyphenols on arachin, with resveratrol and catechin demonstrating the highest binding affinity. Additionally, catechin, myricetin, and gallic acid were observed to form high-molecular-weight polymers with arachin through covalent cross-linking, verified by SDS-PAGE. Fourier infrared spectroscopy showed that polyphenols induced various conformational changes in arachin, notably increasing the proportion of α-helix, with the maximum rise reaching 36.33%. The polyphenols decreased the surface hydrophobicity and solubility of arachin, with the lowest levels dropping to 19.92 µg and 51.7%, respectively. In conclusion, the interaction between the eight polyphenols and arachin induced diverse alterations to the conformation and physicochemical properties of arachin. This study offers a scientific basis and theoretical guidance for the further processing and utilization of polyphenols and arachin. |
| format | Article |
| id | doaj-art-87d29bd571a34615a217765e81a2c2e0 |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-87d29bd571a34615a217765e81a2c2e02025-01-10T06:49:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-01-01462748210.13386/j.issn1002-0306.20240202082024020208-2Research on the Interaction between Eight Polyphenols and ArachinZuwei ZHANG0Rurui LI1Cong YANG2Yongyan MENG3Mingjuan SHEN4Jian SUN5Xuechun ZHANG6College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaThis study investigated the interaction between arachin and eight common polyphenols—ferulic acid, resveratrol, hesperidin, catechin, quercetin, curcumin, gallic acid, and myricetin. The interaction patterns of these polyphenols with arachin were analyzed using UV-visible and fluorescence spectroscopy, with a focus on their effects on protein structure, surface hydrophobicity and solubility. UV-visible spectroscopy revealed that the polyphenols altered the microenvironment surrounding the amino acid residues of arachin, leading to changes in its molecular conformation. Fluorescence spectroscopy identified static quenching as the quenching mechanism of polyphenols on arachin, with resveratrol and catechin demonstrating the highest binding affinity. Additionally, catechin, myricetin, and gallic acid were observed to form high-molecular-weight polymers with arachin through covalent cross-linking, verified by SDS-PAGE. Fourier infrared spectroscopy showed that polyphenols induced various conformational changes in arachin, notably increasing the proportion of α-helix, with the maximum rise reaching 36.33%. The polyphenols decreased the surface hydrophobicity and solubility of arachin, with the lowest levels dropping to 19.92 µg and 51.7%, respectively. In conclusion, the interaction between the eight polyphenols and arachin induced diverse alterations to the conformation and physicochemical properties of arachin. This study offers a scientific basis and theoretical guidance for the further processing and utilization of polyphenols and arachin.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020208arachinpolyphenolinteractionspectrumstructure |
| spellingShingle | Zuwei ZHANG Rurui LI Cong YANG Yongyan MENG Mingjuan SHEN Jian SUN Xuechun ZHANG Research on the Interaction between Eight Polyphenols and Arachin Shipin gongye ke-ji arachin polyphenol interaction spectrum structure |
| title | Research on the Interaction between Eight Polyphenols and Arachin |
| title_full | Research on the Interaction between Eight Polyphenols and Arachin |
| title_fullStr | Research on the Interaction between Eight Polyphenols and Arachin |
| title_full_unstemmed | Research on the Interaction between Eight Polyphenols and Arachin |
| title_short | Research on the Interaction between Eight Polyphenols and Arachin |
| title_sort | research on the interaction between eight polyphenols and arachin |
| topic | arachin polyphenol interaction spectrum structure |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020208 |
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