Rational Design of a Truncated β-Galactosidase from Bacillus sp. and Its Application in the Synthesis of Lacto-N-neotetraose

Rational Design of a Truncated β-Galactosidase from Bacillus sp. and Its Application in the Synthesis of Lacto-N-neotetraose

Objective: A truncated β-galactosidase (BsGal95-D) from Bacillus sp. S3 was biochemically characterized, engineered through rational design, and applied in lacto-N-neotetraose (LNnT) synthesis. Methods: The truncated β-galactosidase gene (BsGal95-D) from Bacillus sp. S3 was cloned and expressed in E...

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Bibliographic Details
Main Authors: Jianyu WANG, Zhixuan XIANG, Dan LIU, Zhengqiang JIANG, Qiaojuan YAN
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-09-01
Series:Shipin gongye ke-ji
Subjects:
β-galactosidase
bacillus sp.
rational design
lacto-n-neotetraose
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024100378
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