Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq
Background: Human bocavirus (HBoV) typically affects adolescents and causes upper and lower respiratory infections. However, little is known about the clinical features of this pathogen and its implications for respiratory infections in adults. Objectives: To detect the HBoV effect on adults and ado...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-10-01
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| Series: | Medical Journal of Babylon |
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| Online Access: | https://doi.org/10.4103/MJBL.MJBL_1265_23 |
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| author | Anmar A. AlTaie Noor Raad Abdulghany Muhammad Abdul-Ghani Muhammad Mohammad M. Salih Iman Mutasher Aufi |
| author_facet | Anmar A. AlTaie Noor Raad Abdulghany Muhammad Abdul-Ghani Muhammad Mohammad M. Salih Iman Mutasher Aufi |
| author_sort | Anmar A. AlTaie |
| collection | DOAJ |
| description | Background: Human bocavirus (HBoV) typically affects adolescents and causes upper and lower respiratory infections. However, little is known about the clinical features of this pathogen and its implications for respiratory infections in adults. Objectives: To detect the HBoV effect on adults and adolescents causing respiratory diseases in the lower respiratory tract. Materials and Methods: Seventy throat and nasopharyngeal swabs were collected from male and female patients, aged 11–57 years, from three hospitals in Mosul City, Iraq, within 6 months in 2024. The samples were stored in a viral transport medium until indirect enzyme-linked immunosorbent assay and real-time polymerase chain reaction (RT-PCR) were performed. Results: Out of 70 samples, the prevalence rate of immunoglobulin (Ig)M antibodies was 37 (53%), IgG was 33 (47%), and viral DNA for HBoV was 24 (34%), respectively. The dominating age groups were above 30 years, and males were more susceptible to infection compared with females. Conclusion: The best diagnostic method for the detection of HBoV is RT-PCR. The present study highlights RT-PCR in diagnosing respiratory infections. This technique can be compared with other immunological methods to determine the severity of the disease. |
| format | Article |
| id | doaj-art-8789b9abb7d2433480053deadb3a3fc8 |
| institution | Kabale University |
| issn | 1812-156X 2312-6760 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Babylon |
| spelling | doaj-art-8789b9abb7d2433480053deadb3a3fc82025-01-14T11:24:47ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602024-10-0121Suppl 2S177S18110.4103/MJBL.MJBL_1265_23Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, IraqAnmar A. AlTaieNoor Raad AbdulghanyMuhammad Abdul-Ghani MuhammadMohammad M. SalihIman Mutasher AufiBackground: Human bocavirus (HBoV) typically affects adolescents and causes upper and lower respiratory infections. However, little is known about the clinical features of this pathogen and its implications for respiratory infections in adults. Objectives: To detect the HBoV effect on adults and adolescents causing respiratory diseases in the lower respiratory tract. Materials and Methods: Seventy throat and nasopharyngeal swabs were collected from male and female patients, aged 11–57 years, from three hospitals in Mosul City, Iraq, within 6 months in 2024. The samples were stored in a viral transport medium until indirect enzyme-linked immunosorbent assay and real-time polymerase chain reaction (RT-PCR) were performed. Results: Out of 70 samples, the prevalence rate of immunoglobulin (Ig)M antibodies was 37 (53%), IgG was 33 (47%), and viral DNA for HBoV was 24 (34%), respectively. The dominating age groups were above 30 years, and males were more susceptible to infection compared with females. Conclusion: The best diagnostic method for the detection of HBoV is RT-PCR. The present study highlights RT-PCR in diagnosing respiratory infections. This technique can be compared with other immunological methods to determine the severity of the disease.https://doi.org/10.4103/MJBL.MJBL_1265_23bocaviruselisafast-track respiratory pathogens 33real-time pcrrespiratory tract infections |
| spellingShingle | Anmar A. AlTaie Noor Raad Abdulghany Muhammad Abdul-Ghani Muhammad Mohammad M. Salih Iman Mutasher Aufi Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq Medical Journal of Babylon bocavirus elisa fast-track respiratory pathogens 33 real-time pcr respiratory tract infections |
| title | Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq |
| title_full | Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq |
| title_fullStr | Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq |
| title_full_unstemmed | Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq |
| title_short | Immunological and Molecular Method for the Diagnosis of Human Bocavirus in Patients with Respiratory Infections in Mosul, Iraq |
| title_sort | immunological and molecular method for the diagnosis of human bocavirus in patients with respiratory infections in mosul iraq |
| topic | bocavirus elisa fast-track respiratory pathogens 33 real-time pcr respiratory tract infections |
| url | https://doi.org/10.4103/MJBL.MJBL_1265_23 |
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