Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy
The impressive increase in antimicrobial resistance has required the development of alternative treatments that act on multiple non-specific molecular targets and are effective against a broad range of microorganisms. Antimicrobial Photodynamic Therapy (aPDT) is based on microbial inactivation from...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Photochemistry and Photobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666469024000277 |
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| author | Isabelle Almeida de Lima Lorraine Gabriele Fiuza Johan Sebastián Díaz Tovar Dianeth Sara Lima Bejar Ana Julia Barbosa Tomé Michelle Barreto Requena Layla Pires Gang Zheng Natalia Mayumi Inada Cristina Kurachi Vanderlei Salvador Bagnato |
| author_facet | Isabelle Almeida de Lima Lorraine Gabriele Fiuza Johan Sebastián Díaz Tovar Dianeth Sara Lima Bejar Ana Julia Barbosa Tomé Michelle Barreto Requena Layla Pires Gang Zheng Natalia Mayumi Inada Cristina Kurachi Vanderlei Salvador Bagnato |
| author_sort | Isabelle Almeida de Lima |
| collection | DOAJ |
| description | The impressive increase in antimicrobial resistance has required the development of alternative treatments that act on multiple non-specific molecular targets and are effective against a broad range of microorganisms. Antimicrobial Photodynamic Therapy (aPDT) is based on microbial inactivation from oxidative stress and represents an important tool for inactivating microorganisms with low risk of resistance selection. Therefore, our research group has been devoted to demonstrating its effectiveness against pathogens that cause pneumonia, one of the most lethal infections worldwide. Previous studies reported the efficiency and safety of an in vitro photoinactivation protocol for Streptococcus pneumoniae and the delivery of infrared light (external illumination) and photosensitizer (PS) in an animal model. However, the in vivo inactivation of microorganisms still poses challenges due to the presence of lung surfactant (LS), which traps PSs, preventing them from reaching the microbial target. This study investigated different approaches such as use of emulsifiers, perfluorocarbon, oxygen nanobubbles, and copolymer towards overcoming LS and optimizing aPDT response. The most promising strategy consisted in combining indocyanine green (ICG) with GantrezTM AN-139 - a Polyvinyl Methyl Ether/Maleic Anhydride copolymer (PVM/MA) – showing high microbial inactivation and safety for human lung epithelial (A549) and fibroblast (MRC-9) cell lines. The in vitro experiments provided an alternative to overcome the limited PS distribution through LS and will serve as the basis for in vivo studies. |
| format | Article |
| id | doaj-art-87744e4b05594dfeb713eb3e3f1a0706 |
| institution | Kabale University |
| issn | 2666-4690 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Photochemistry and Photobiology |
| spelling | doaj-art-87744e4b05594dfeb713eb3e3f1a07062024-11-29T06:25:15ZengElsevierJournal of Photochemistry and Photobiology2666-46902024-12-0124100252Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapyIsabelle Almeida de Lima0Lorraine Gabriele Fiuza1Johan Sebastián Díaz Tovar2Dianeth Sara Lima Bejar3Ana Julia Barbosa Tomé4Michelle Barreto Requena5Layla Pires6Gang Zheng7Natalia Mayumi Inada8Cristina Kurachi9Vanderlei Salvador Bagnato10São Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil; Corresponding author.São Carlos Institute of Physics, University of São Paulo, São Carlos, SP, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, SP, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, SP, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, SP, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil; Department of Biomedical Engineering, Texas A&M University, USAPrincess Margaret Cancer Centre, University Health Network, Toronto, CanadaPrincess Margaret Cancer Centre, University Health Network, Toronto, CanadaSão Carlos Institute of Physics, University of São Paulo, São Carlos, SP, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, SP, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil; Department of Biomedical Engineering, Texas A&M University, USAThe impressive increase in antimicrobial resistance has required the development of alternative treatments that act on multiple non-specific molecular targets and are effective against a broad range of microorganisms. Antimicrobial Photodynamic Therapy (aPDT) is based on microbial inactivation from oxidative stress and represents an important tool for inactivating microorganisms with low risk of resistance selection. Therefore, our research group has been devoted to demonstrating its effectiveness against pathogens that cause pneumonia, one of the most lethal infections worldwide. Previous studies reported the efficiency and safety of an in vitro photoinactivation protocol for Streptococcus pneumoniae and the delivery of infrared light (external illumination) and photosensitizer (PS) in an animal model. However, the in vivo inactivation of microorganisms still poses challenges due to the presence of lung surfactant (LS), which traps PSs, preventing them from reaching the microbial target. This study investigated different approaches such as use of emulsifiers, perfluorocarbon, oxygen nanobubbles, and copolymer towards overcoming LS and optimizing aPDT response. The most promising strategy consisted in combining indocyanine green (ICG) with GantrezTM AN-139 - a Polyvinyl Methyl Ether/Maleic Anhydride copolymer (PVM/MA) – showing high microbial inactivation and safety for human lung epithelial (A549) and fibroblast (MRC-9) cell lines. The in vitro experiments provided an alternative to overcome the limited PS distribution through LS and will serve as the basis for in vivo studies.http://www.sciencedirect.com/science/article/pii/S2666469024000277PneumoniaLung surfactantIndocyanine greenInfrared lightAntimicrobial photodynamic therapy |
| spellingShingle | Isabelle Almeida de Lima Lorraine Gabriele Fiuza Johan Sebastián Díaz Tovar Dianeth Sara Lima Bejar Ana Julia Barbosa Tomé Michelle Barreto Requena Layla Pires Gang Zheng Natalia Mayumi Inada Cristina Kurachi Vanderlei Salvador Bagnato Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy Journal of Photochemistry and Photobiology Pneumonia Lung surfactant Indocyanine green Infrared light Antimicrobial photodynamic therapy |
| title | Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy |
| title_full | Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy |
| title_fullStr | Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy |
| title_full_unstemmed | Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy |
| title_short | Strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy |
| title_sort | strategies for overcoming the lung surfactant barrier and achieving success in antimicrobial photodynamic therapy |
| topic | Pneumonia Lung surfactant Indocyanine green Infrared light Antimicrobial photodynamic therapy |
| url | http://www.sciencedirect.com/science/article/pii/S2666469024000277 |
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