Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification
A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural protein corona on natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix the natural corona on “biological nanoparticles”...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Beilstein-Institut
2024-12-01
|
| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.15.130 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841557363649150976 |
|---|---|
| author | Julia E. Poletaeva Anastasiya V. Tupitsyna Alina E. Grigor’eva Ilya S. Dovydenko Elena I. Ryabchikova |
| author_facet | Julia E. Poletaeva Anastasiya V. Tupitsyna Alina E. Grigor’eva Ilya S. Dovydenko Elena I. Ryabchikova |
| author_sort | Julia E. Poletaeva |
| collection | DOAJ |
| description | A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural protein corona on natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix the natural corona on “biological nanoparticles” (bio-NPs) in fetal bovine serum and newborn bovine serum; native sera served as a control. To isolate photomodified bio-NPs, we used ultracentrifugation (UC), sucrose gradient (12%, 30%, and 50%), and sucrose cushion (30%) methods. Isolated bio-NPs were visualized using transmission electron microscopy. The results showed that a protein corona is present on extracellular vesicles and lipoproteins isolated by UC. The isolation of bio-NPs through sucrose gradient or cushion did not preserve the protein corona. At the same time, we observed signs of a negative effect of the sucrose gradient on bio-NPs of intact and photomodified serum; when isolating these particles on a sucrose cushion, no negative effects were observed. We believe that the data we present will be useful to researchers using sucrose solutions to isolate bio-NPs and working on the properties of the protein corona. In this work, we have obtained direct images of a “natural” protein corona on natural bio-NPs of blood serum for the first time |
| format | Article |
| id | doaj-art-874fbb23bcec47f2b0178b2b12ae6e71 |
| institution | Kabale University |
| issn | 2190-4286 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj-art-874fbb23bcec47f2b0178b2b12ae6e712025-01-06T12:27:48ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862024-12-011511654166610.3762/bjnano.15.1302190-4286-15-130Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodificationJulia E. Poletaeva0Anastasiya V. Tupitsyna1Alina E. Grigor’eva2Ilya S. Dovydenko3Elena I. Ryabchikova4Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av., 8, Novosibirsk, 630090, Russian Federation Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av., 8, Novosibirsk, 630090, Russian Federation Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av., 8, Novosibirsk, 630090, Russian Federation Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av., 8, Novosibirsk, 630090, Russian Federation Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av., 8, Novosibirsk, 630090, Russian Federation A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural protein corona on natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix the natural corona on “biological nanoparticles” (bio-NPs) in fetal bovine serum and newborn bovine serum; native sera served as a control. To isolate photomodified bio-NPs, we used ultracentrifugation (UC), sucrose gradient (12%, 30%, and 50%), and sucrose cushion (30%) methods. Isolated bio-NPs were visualized using transmission electron microscopy. The results showed that a protein corona is present on extracellular vesicles and lipoproteins isolated by UC. The isolation of bio-NPs through sucrose gradient or cushion did not preserve the protein corona. At the same time, we observed signs of a negative effect of the sucrose gradient on bio-NPs of intact and photomodified serum; when isolating these particles on a sucrose cushion, no negative effects were observed. We believe that the data we present will be useful to researchers using sucrose solutions to isolate bio-NPs and working on the properties of the protein corona. In this work, we have obtained direct images of a “natural” protein corona on natural bio-NPs of blood serum for the first timehttps://doi.org/10.3762/bjnano.15.130chylomicronsextracellular vesicleslipoproteinsphotomodificationprotein corona |
| spellingShingle | Julia E. Poletaeva Anastasiya V. Tupitsyna Alina E. Grigor’eva Ilya S. Dovydenko Elena I. Ryabchikova Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification Beilstein Journal of Nanotechnology chylomicrons extracellular vesicles lipoproteins photomodification protein corona |
| title | Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification |
| title_full | Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification |
| title_fullStr | Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification |
| title_full_unstemmed | Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification |
| title_short | Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification |
| title_sort | attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification |
| topic | chylomicrons extracellular vesicles lipoproteins photomodification protein corona |
| url | https://doi.org/10.3762/bjnano.15.130 |
| work_keys_str_mv | AT juliaepoletaeva attemptstopreserveandvisualizeproteincoronaonthesurfaceofbiologicalnanoparticlesinbloodserumusingphotomodification AT anastasiyavtupitsyna attemptstopreserveandvisualizeproteincoronaonthesurfaceofbiologicalnanoparticlesinbloodserumusingphotomodification AT alinaegrigoreva attemptstopreserveandvisualizeproteincoronaonthesurfaceofbiologicalnanoparticlesinbloodserumusingphotomodification AT ilyasdovydenko attemptstopreserveandvisualizeproteincoronaonthesurfaceofbiologicalnanoparticlesinbloodserumusingphotomodification AT elenairyabchikova attemptstopreserveandvisualizeproteincoronaonthesurfaceofbiologicalnanoparticlesinbloodserumusingphotomodification |