Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traits
During the course of our ongoing study of the diversity of Thai fungi and their secondary metabolites, numerous specimens within the Hypoxylaceae have been characterised by traditional morphology, chemotaxonomy using ultra-high-performance liquid chromatography coupled to diode array detection and i...
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| Language: | English |
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Taylor & Francis Group
2025-01-01
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| Series: | Mycology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21501203.2024.2435979 |
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| author | Sarunyou Wongkanoun Esteban Charria-Girón Marjorie Cedeño-Sanchez Boonchuai Chainuwong Sayanh Somrithipol Eric Kuhnert Prasert Srikitikulchai Natapol Pornputtapong Frank Surup Jennifer Luangsa-ard Marc Stadler |
| author_facet | Sarunyou Wongkanoun Esteban Charria-Girón Marjorie Cedeño-Sanchez Boonchuai Chainuwong Sayanh Somrithipol Eric Kuhnert Prasert Srikitikulchai Natapol Pornputtapong Frank Surup Jennifer Luangsa-ard Marc Stadler |
| author_sort | Sarunyou Wongkanoun |
| collection | DOAJ |
| description | During the course of our ongoing study of the diversity of Thai fungi and their secondary metabolites, numerous specimens within the Hypoxylaceae have been characterised by traditional morphology, chemotaxonomy using ultra-high-performance liquid chromatography coupled to diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS), and molecular phylogenetic analyses. MS/MS-based analysis of the major stromatal metabolites of a newly identified taxon, Hypoxylon luteogranulatum, indicated the production of distinct compounds compared to the azaphilone and binaphthalene pigments commonly found in the Hypoxylaceae, aside from the presence of the chemotaxonomic marker binaphthalene tetrol (BNT). Further analysis suggested that one of the major metabolites had the molecular formula C13H13NO3, identical to hypoxyvermelhotin A, a yellow pigment so far exclusively found in Hypoxylon lechatii. Its identity was confirmed after purification by preparative high-performance liquid chromatography with nuclear magnetic resonance (NMR) data, and genome analysis of H. lechatii revealed the presence of different hybrid polyketide synthases-non-ribosomal peptide synthetases (PKS-NRPS) hybrid clusters. Despite chemotaxonomic similarities with H. lechatii, we propose a new species, which is morphologically distinct from H. lechatii. Our molecular phylogenetic study provides substantial evidence distinguishing H. luteogranulatum clearly from H. lechatii and allied members within the Hypoxylaceae. Additionally, future studies are needed to better understand the ecological behaviour of H. luteogranulatum and identify the ecological role of the vermelhotin-like molecules within this putative interaction. |
| format | Article |
| id | doaj-art-8736bf94bffd4485b8d88f00fc4f0932 |
| institution | Kabale University |
| issn | 2150-1203 2150-1211 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj-art-8736bf94bffd4485b8d88f00fc4f09322025-01-16T08:22:24ZengTaylor & Francis GroupMycology2150-12032150-12112025-01-0111910.1080/21501203.2024.2435979Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traitsSarunyou Wongkanoun0Esteban Charria-Girón1Marjorie Cedeño-Sanchez2Boonchuai Chainuwong3Sayanh Somrithipol4Eric Kuhnert5Prasert Srikitikulchai6Natapol Pornputtapong7Frank Surup8Jennifer Luangsa-ard9Marc Stadler10Department of Biochemistry and Microbiology, Center of Excellence for DNA Barcoding of Thai Medicinal Plants, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, ThailandDepartment of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), German Centre for Infection Research Association (DZIF), Braunschweig, GermanyDepartment of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), German Centre for Infection Research Association (DZIF), Braunschweig, GermanyNational Biobank of Thailand (NBT), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani, ThailandPlant Microbe Interaction Research Team (APMT), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani, ThailandGinkgo Bioworks, Inc., Boston, USANational Biobank of Thailand (NBT), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani, ThailandDepartment of Biochemistry and Microbiology, Center of Excellence for DNA Barcoding of Thai Medicinal Plants, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, ThailandDepartment of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), German Centre for Infection Research Association (DZIF), Braunschweig, GermanyPlant Microbe Interaction Research Team (APMT), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani, ThailandDepartment of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), German Centre for Infection Research Association (DZIF), Braunschweig, GermanyDuring the course of our ongoing study of the diversity of Thai fungi and their secondary metabolites, numerous specimens within the Hypoxylaceae have been characterised by traditional morphology, chemotaxonomy using ultra-high-performance liquid chromatography coupled to diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS), and molecular phylogenetic analyses. MS/MS-based analysis of the major stromatal metabolites of a newly identified taxon, Hypoxylon luteogranulatum, indicated the production of distinct compounds compared to the azaphilone and binaphthalene pigments commonly found in the Hypoxylaceae, aside from the presence of the chemotaxonomic marker binaphthalene tetrol (BNT). Further analysis suggested that one of the major metabolites had the molecular formula C13H13NO3, identical to hypoxyvermelhotin A, a yellow pigment so far exclusively found in Hypoxylon lechatii. Its identity was confirmed after purification by preparative high-performance liquid chromatography with nuclear magnetic resonance (NMR) data, and genome analysis of H. lechatii revealed the presence of different hybrid polyketide synthases-non-ribosomal peptide synthetases (PKS-NRPS) hybrid clusters. Despite chemotaxonomic similarities with H. lechatii, we propose a new species, which is morphologically distinct from H. lechatii. Our molecular phylogenetic study provides substantial evidence distinguishing H. luteogranulatum clearly from H. lechatii and allied members within the Hypoxylaceae. Additionally, future studies are needed to better understand the ecological behaviour of H. luteogranulatum and identify the ecological role of the vermelhotin-like molecules within this putative interaction.https://www.tandfonline.com/doi/10.1080/21501203.2024.2435979Phylogenytetramic acidsmetabolomicsgenome miningSordariomycetes: new speciesXylariales |
| spellingShingle | Sarunyou Wongkanoun Esteban Charria-Girón Marjorie Cedeño-Sanchez Boonchuai Chainuwong Sayanh Somrithipol Eric Kuhnert Prasert Srikitikulchai Natapol Pornputtapong Frank Surup Jennifer Luangsa-ard Marc Stadler Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traits Mycology Phylogeny tetramic acids metabolomics genome mining Sordariomycetes: new species Xylariales |
| title | Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traits |
| title_full | Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traits |
| title_fullStr | Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traits |
| title_full_unstemmed | Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traits |
| title_short | Hypoxylon luteogranulatum (Hypoxylaceae, Xylariales), a novel species from Thailand with distinct chemical and ecological traits |
| title_sort | hypoxylon luteogranulatum hypoxylaceae xylariales a novel species from thailand with distinct chemical and ecological traits |
| topic | Phylogeny tetramic acids metabolomics genome mining Sordariomycetes: new species Xylariales |
| url | https://www.tandfonline.com/doi/10.1080/21501203.2024.2435979 |
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