Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective
Introduction: Muntingia calabura is a medicinal plant possessing antimicrobial properties against various bacteria. The purpose of this study was to examine in vitro and in silico activity of the ethyl acetate fraction of M. calabura leaves against the acne-causing commensal bacterium, Staphylococcu...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahrekord University of Medical Sciences
2024-04-01
|
| Series: | Journal of HerbMed Pharmacology |
| Subjects: | |
| Online Access: | https://herbmedpharmacol.com/PDF/jhp-13-240.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846142657003257856 |
|---|---|
| author | Aloysius Imanadi Sambi Bakti Wahyu Saputra Agustina Setiawati |
| author_facet | Aloysius Imanadi Sambi Bakti Wahyu Saputra Agustina Setiawati |
| author_sort | Aloysius Imanadi Sambi |
| collection | DOAJ |
| description | Introduction: Muntingia calabura is a medicinal plant possessing antimicrobial properties against various bacteria. The purpose of this study was to examine in vitro and in silico activity of the ethyl acetate fraction of M. calabura leaves against the acne-causing commensal bacterium, Staphylococcus epidermidis. Methods: In this study, M. calabura leaves were extracted using ethanol and then further fractionated with ethyl acetate. The phytochemicals in the fraction were identified with thin layer chromatography (TLC). The activity of the fraction was then tested in S. epidermidis culture using the agar diffusion method. Additionally, the molecular docking of M. calabura phytochemicals constituents was simulated to teicoplanin-associated locus regulator (TcaR) of S. epidermidis. Results: The ethyl acetate fraction of M. calabura exhibited robust antibacterial activity against S. epidermidis culture, resulting in inhibition zones ranging from 5 to 10 mm. The fraction was found to contain flavonoids, saponins, and tannins as identified constituents. Further, during the molecular docking analysis, stigmasterol and 7-methoxyflavone demonstrated binding to TcaR with a lower and comparable binding energy of -7.40 and -6.19 kcal/mol, respectively, compared to the control drug, Penicillin-G (-6.40 kcal/mol). Conclusion: M. calabura has the potential to serve as a valuable source of active phytochemical compounds for addressing acne. Further studies are needed to isolate and evaluate each compound found in M. calabura individually against S. epidermidis. |
| format | Article |
| id | doaj-art-86ce176556804934b3c3d07b0fdee098 |
| institution | Kabale University |
| issn | 2345-5004 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj-art-86ce176556804934b3c3d07b0fdee0982024-12-03T07:34:27ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042024-04-0113224024810.34172/jhp.2024.48170jhp-48170Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspectiveAloysius Imanadi Sambi0Bakti Wahyu Saputra1Agustina Setiawati2Faculty of Pharmacy, Sanata Dharma University, Sleman, Yogyakarta 55284, IndonesiaFaculty of Pharmacy, Sanata Dharma University, Sleman, Yogyakarta 55284, IndonesiaFaculty of Pharmacy, Sanata Dharma University, Sleman, Yogyakarta 55284, IndonesiaIntroduction: Muntingia calabura is a medicinal plant possessing antimicrobial properties against various bacteria. The purpose of this study was to examine in vitro and in silico activity of the ethyl acetate fraction of M. calabura leaves against the acne-causing commensal bacterium, Staphylococcus epidermidis. Methods: In this study, M. calabura leaves were extracted using ethanol and then further fractionated with ethyl acetate. The phytochemicals in the fraction were identified with thin layer chromatography (TLC). The activity of the fraction was then tested in S. epidermidis culture using the agar diffusion method. Additionally, the molecular docking of M. calabura phytochemicals constituents was simulated to teicoplanin-associated locus regulator (TcaR) of S. epidermidis. Results: The ethyl acetate fraction of M. calabura exhibited robust antibacterial activity against S. epidermidis culture, resulting in inhibition zones ranging from 5 to 10 mm. The fraction was found to contain flavonoids, saponins, and tannins as identified constituents. Further, during the molecular docking analysis, stigmasterol and 7-methoxyflavone demonstrated binding to TcaR with a lower and comparable binding energy of -7.40 and -6.19 kcal/mol, respectively, compared to the control drug, Penicillin-G (-6.40 kcal/mol). Conclusion: M. calabura has the potential to serve as a valuable source of active phytochemical compounds for addressing acne. Further studies are needed to isolate and evaluate each compound found in M. calabura individually against S. epidermidis.https://herbmedpharmacol.com/PDF/jhp-13-240.pdfantimicrobial agentnatural productsdrug discoveryteicoplanin-associated locus regulatormolecular docking |
| spellingShingle | Aloysius Imanadi Sambi Bakti Wahyu Saputra Agustina Setiawati Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective Journal of HerbMed Pharmacology antimicrobial agent natural products drug discovery teicoplanin-associated locus regulator molecular docking |
| title | Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective |
| title_full | Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective |
| title_fullStr | Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective |
| title_full_unstemmed | Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective |
| title_short | Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective |
| title_sort | exploring the anti acne potential of muntingia calabura l leaves against staphylococcus epidermidis in vitro and in silico perspective |
| topic | antimicrobial agent natural products drug discovery teicoplanin-associated locus regulator molecular docking |
| url | https://herbmedpharmacol.com/PDF/jhp-13-240.pdf |
| work_keys_str_mv | AT aloysiusimanadisambi exploringtheantiacnepotentialofmuntingiacalaburalleavesagainststaphylococcusepidermidisinvitroandinsilicoperspective AT baktiwahyusaputra exploringtheantiacnepotentialofmuntingiacalaburalleavesagainststaphylococcusepidermidisinvitroandinsilicoperspective AT agustinasetiawati exploringtheantiacnepotentialofmuntingiacalaburalleavesagainststaphylococcusepidermidisinvitroandinsilicoperspective |