Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum
IntroductionChronic inflammation caused by Escherichia coli infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of ear...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2024.1523098/full |
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| author | Karol Mierzejewski Robert Stryiński Iwona Bogacka Monika Golubska Mónica Carrera Aleksandra Kurzynska |
| author_facet | Karol Mierzejewski Robert Stryiński Iwona Bogacka Monika Golubska Mónica Carrera Aleksandra Kurzynska |
| author_sort | Karol Mierzejewski |
| collection | DOAJ |
| description | IntroductionChronic inflammation caused by Escherichia coli infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of early pregnancy through the secretion of progesterone. Understanding the intricate interplay between inflammatory processes and reproductive organ’s function is crucial for the development of effective therapeutic strategies to alleviate reproductive disorders and improve fertility.MethodsThe aim of this study was to determine the in vitro effects of lipopolysaccharide (LPS) on the proteomic profile of the porcine CL in the mid-luteal phase of the estrous cycle using LC-MS/MS analysis. The CL slices were incubated in the presence of LPS for 24 h.ResultsWe identified 12 differentially regulated proteins after treatment with LPS (7 of them were upregulated, while 5 were downregulated). The analysis showed that these proteins are involved in processes such as glucose metabolism, the tricarboxylic acid cycle (TCA), detoxification processes as well as steroid biosynthesis in the CL. Moreover, we demonstrated that LPS decreases glucose levels and increases progesterone levels in the CL.ConclusionThese findings suggest that LPS modulates key metabolic pathways in the CL, potentially impacting its functional activity. |
| format | Article |
| id | doaj-art-868bde5dab0c4cd09d7002dc0c7dc620 |
| institution | Kabale University |
| issn | 2296-889X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj-art-868bde5dab0c4cd09d7002dc0c7dc6202025-01-07T06:40:27ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2025-01-011110.3389/fmolb.2024.15230981523098Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteumKarol Mierzejewski0Robert Stryiński1Iwona Bogacka2Monika Golubska3Mónica Carrera4Aleksandra Kurzynska5Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Food Technology, Institute of Marine Research (IIM), Spanish National Research Council (CSIC), Vigo, SpainDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandIntroductionChronic inflammation caused by Escherichia coli infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of early pregnancy through the secretion of progesterone. Understanding the intricate interplay between inflammatory processes and reproductive organ’s function is crucial for the development of effective therapeutic strategies to alleviate reproductive disorders and improve fertility.MethodsThe aim of this study was to determine the in vitro effects of lipopolysaccharide (LPS) on the proteomic profile of the porcine CL in the mid-luteal phase of the estrous cycle using LC-MS/MS analysis. The CL slices were incubated in the presence of LPS for 24 h.ResultsWe identified 12 differentially regulated proteins after treatment with LPS (7 of them were upregulated, while 5 were downregulated). The analysis showed that these proteins are involved in processes such as glucose metabolism, the tricarboxylic acid cycle (TCA), detoxification processes as well as steroid biosynthesis in the CL. Moreover, we demonstrated that LPS decreases glucose levels and increases progesterone levels in the CL.ConclusionThese findings suggest that LPS modulates key metabolic pathways in the CL, potentially impacting its functional activity.https://www.frontiersin.org/articles/10.3389/fmolb.2024.1523098/fullinflammationmid-luteal phasepigglucose metabolismprogesteronesteroidogenesis |
| spellingShingle | Karol Mierzejewski Robert Stryiński Iwona Bogacka Monika Golubska Mónica Carrera Aleksandra Kurzynska Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum Frontiers in Molecular Biosciences inflammation mid-luteal phase pig glucose metabolism progesterone steroidogenesis |
| title | Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum |
| title_full | Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum |
| title_fullStr | Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum |
| title_full_unstemmed | Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum |
| title_short | Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum |
| title_sort | lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum |
| topic | inflammation mid-luteal phase pig glucose metabolism progesterone steroidogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2024.1523098/full |
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