Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer

Spherical gold/polyacrylic acid (Au/PAA) polymer–inorganic Janus nanoparticles (JNPs) with simultaneous therapeutic and targeting functions were fabricated. The obtained Au/PAA JNPs were further selectively functionalized with folic acid (FA) and thiol PEG amine (SH-PEG-NH2) on Au sides to provide s...

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Main Authors: Hanieh Montaseri, Heidi Abrahamse
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Artificial Cells, Nanomedicine, and Biotechnology
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Online Access:https://www.tandfonline.com/doi/10.1080/21691401.2024.2347369
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author Hanieh Montaseri
Heidi Abrahamse
author_facet Hanieh Montaseri
Heidi Abrahamse
author_sort Hanieh Montaseri
collection DOAJ
description Spherical gold/polyacrylic acid (Au/PAA) polymer–inorganic Janus nanoparticles (JNPs) with simultaneous therapeutic and targeting functions were fabricated. The obtained Au/PAA JNPs were further selectively functionalized with folic acid (FA) and thiol PEG amine (SH-PEG-NH2) on Au sides to provide superior biocompatibility and active targeting, while the other PAA sides were loaded with 5-aminolevulinic acid (5-ALA) to serve as a photosensitizer (PS) for photodynamic therapeutic (PDT) effects on MCF-7 cancer cells. The PS loading of 5-ALA was found to be 83% with an average hydrodynamic size and z-potential of 146 ± 0.8 nm and −6.40 mV respectively for FA-Au/PAA-ALA JNPs. The in vitro PDT study of the JNPs on MCF-7 breast cancer cells under 636 nm laser irradiation indicated the cell viability of 24.7% ± 0.5 for FA-Au/PAA-ALA JNPs at the IC50 value of 0.125 mM. In this regard, the actively targeted FA-Au/PAA-ALA JNPs treatment holds great potential for tumour therapy with high cancer cell-killing efficacy.
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spelling doaj-art-85e7acdb01aa48fa83f3d334895eeaf02024-12-06T10:48:00ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2024-12-0152127027710.1080/21691401.2024.2347369Targeted photodynamic therapy technique of Janus nanoparticles on breast cancerHanieh Montaseri0Heidi Abrahamse1Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South AfricaLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South AfricaSpherical gold/polyacrylic acid (Au/PAA) polymer–inorganic Janus nanoparticles (JNPs) with simultaneous therapeutic and targeting functions were fabricated. The obtained Au/PAA JNPs were further selectively functionalized with folic acid (FA) and thiol PEG amine (SH-PEG-NH2) on Au sides to provide superior biocompatibility and active targeting, while the other PAA sides were loaded with 5-aminolevulinic acid (5-ALA) to serve as a photosensitizer (PS) for photodynamic therapeutic (PDT) effects on MCF-7 cancer cells. The PS loading of 5-ALA was found to be 83% with an average hydrodynamic size and z-potential of 146 ± 0.8 nm and −6.40 mV respectively for FA-Au/PAA-ALA JNPs. The in vitro PDT study of the JNPs on MCF-7 breast cancer cells under 636 nm laser irradiation indicated the cell viability of 24.7% ± 0.5 for FA-Au/PAA-ALA JNPs at the IC50 value of 0.125 mM. In this regard, the actively targeted FA-Au/PAA-ALA JNPs treatment holds great potential for tumour therapy with high cancer cell-killing efficacy.https://www.tandfonline.com/doi/10.1080/21691401.2024.2347369Janus nanoparticlesphotodynamic therapybreast canceractive targeting
spellingShingle Hanieh Montaseri
Heidi Abrahamse
Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer
Artificial Cells, Nanomedicine, and Biotechnology
Janus nanoparticles
photodynamic therapy
breast cancer
active targeting
title Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer
title_full Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer
title_fullStr Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer
title_full_unstemmed Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer
title_short Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer
title_sort targeted photodynamic therapy technique of janus nanoparticles on breast cancer
topic Janus nanoparticles
photodynamic therapy
breast cancer
active targeting
url https://www.tandfonline.com/doi/10.1080/21691401.2024.2347369
work_keys_str_mv AT haniehmontaseri targetedphotodynamictherapytechniqueofjanusnanoparticlesonbreastcancer
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