Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis
Abstract Background The majority of stone formers (87.5–95.9%) exhibit mild to moderate interstitial inflammation surrounding the stone. Neutrophils and neutrophil-derived genes/proteins have been found in renal papillae, stone matrix and urine of calcium oxalate monohydrate (COM) stone formers. How...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | Cell Communication and Signaling |
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| Online Access: | https://doi.org/10.1186/s12964-025-02345-2 |
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| author | Chanettee Lertprapai Paleerath Peerapen Visith Thongboonkerd |
| author_facet | Chanettee Lertprapai Paleerath Peerapen Visith Thongboonkerd |
| author_sort | Chanettee Lertprapai |
| collection | DOAJ |
| description | Abstract Background The majority of stone formers (87.5–95.9%) exhibit mild to moderate interstitial inflammation surrounding the stone. Neutrophils and neutrophil-derived genes/proteins have been found in renal papillae, stone matrix and urine of calcium oxalate monohydrate (COM) stone formers. However, neutrophil-crystal interactions, especially responses of neutrophils to COM crystals, remained unknown. Methods This study addressed the effects of COM crystals on neutrophil cellular proteome and functions, including phagocytosis, activation/degranulation, neutrophil extracellular traps (NETs) formation and reactive oxygen species (ROS) production. Results Label-free quantitative (LFQ) proteomics using nanoLC-ESI-Qq-TOF MS/MS with highly stringent criteria revealed that COM caused altered levels of 22 neutrophil proteins involved mainly in immune responses. Investigating neutrophil innate immune functions using flow cytometry, immunofluorescence/fluorescence imaging, ELISA and dichlorodihydrofluorescein diacetate (DCFH-DA) assay revealed that COM enhanced neutrophil phagocytic activity, NETs formation, activation/degranulation and ROS production. Moreover, secretome (a set of secretory products) from COM-treated neutrophils induced the recruitment of macrophages to phagocytose the COM-treated neutrophils. Conclusions These findings illustrate the expression and functional responses of neutrophils to COM crystals and implicate the important roles that neutrophils play in nephrolithiasis. |
| format | Article |
| id | doaj-art-853f99bd12b94741b4bd9a8e19a2c8c5 |
| institution | Kabale University |
| issn | 1478-811X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj-art-853f99bd12b94741b4bd9a8e19a2c8c52025-08-20T03:43:01ZengBMCCell Communication and Signaling1478-811X2025-07-0123112110.1186/s12964-025-02345-2Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasisChanettee Lertprapai0Paleerath Peerapen1Visith Thongboonkerd2Medical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol UniversityMedical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol UniversityMedical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol UniversityAbstract Background The majority of stone formers (87.5–95.9%) exhibit mild to moderate interstitial inflammation surrounding the stone. Neutrophils and neutrophil-derived genes/proteins have been found in renal papillae, stone matrix and urine of calcium oxalate monohydrate (COM) stone formers. However, neutrophil-crystal interactions, especially responses of neutrophils to COM crystals, remained unknown. Methods This study addressed the effects of COM crystals on neutrophil cellular proteome and functions, including phagocytosis, activation/degranulation, neutrophil extracellular traps (NETs) formation and reactive oxygen species (ROS) production. Results Label-free quantitative (LFQ) proteomics using nanoLC-ESI-Qq-TOF MS/MS with highly stringent criteria revealed that COM caused altered levels of 22 neutrophil proteins involved mainly in immune responses. Investigating neutrophil innate immune functions using flow cytometry, immunofluorescence/fluorescence imaging, ELISA and dichlorodihydrofluorescein diacetate (DCFH-DA) assay revealed that COM enhanced neutrophil phagocytic activity, NETs formation, activation/degranulation and ROS production. Moreover, secretome (a set of secretory products) from COM-treated neutrophils induced the recruitment of macrophages to phagocytose the COM-treated neutrophils. Conclusions These findings illustrate the expression and functional responses of neutrophils to COM crystals and implicate the important roles that neutrophils play in nephrolithiasis.https://doi.org/10.1186/s12964-025-02345-2DegranulationInflammationNeutrophil extracellular trapsPhagocytosisProteomicsReactive oxygen species |
| spellingShingle | Chanettee Lertprapai Paleerath Peerapen Visith Thongboonkerd Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis Cell Communication and Signaling Degranulation Inflammation Neutrophil extracellular traps Phagocytosis Proteomics Reactive oxygen species |
| title | Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis |
| title_full | Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis |
| title_fullStr | Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis |
| title_full_unstemmed | Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis |
| title_short | Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis |
| title_sort | effects of calcium oxalate crystals on neutrophil cellular proteome and functions implications for nephrolithiasis |
| topic | Degranulation Inflammation Neutrophil extracellular traps Phagocytosis Proteomics Reactive oxygen species |
| url | https://doi.org/10.1186/s12964-025-02345-2 |
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