Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion Mechanism
Herein, a novel approach is presented to mitigate the fluorescence interference during the detection of vibrational signal via the stimulated emission depletion (STED). STED is the mechanism commonly employed in optical imaging; however, its application should not be confined solely to this field. T...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-01-01
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| Series: | Advanced Photonics Research |
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| Online Access: | https://doi.org/10.1002/adpr.202400078 |
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| author | Ge Wang Lizhen Chen Qiuqiang Zhan Guangyou Fang Yisen Wang |
| author_facet | Ge Wang Lizhen Chen Qiuqiang Zhan Guangyou Fang Yisen Wang |
| author_sort | Ge Wang |
| collection | DOAJ |
| description | Herein, a novel approach is presented to mitigate the fluorescence interference during the detection of vibrational signal via the stimulated emission depletion (STED). STED is the mechanism commonly employed in optical imaging; however, its application should not be confined solely to this field. To explore additional possibilities, a novel application of STED in vibrational spectroscopy detection is introduced. Vibrational spectroscopy is a widely used technique for the material detection and identification, but its sensitivity is influenced by impurity signals, especially the fluorescence. The proposed method is capable of suppressing fluorescence without influencing vibrational signal. At the low concentration of fluorescent impurities, the signal‐to‐background ratio of vibrational spectroscopy is 2.6 times as high as that without this method. The introduction of depletion light can enhance the detection of vibrational signals, resulting in more optimal signal detection. A promising new application of STED other than super‐resolution imaging is investigated. |
| format | Article |
| id | doaj-art-852819130eca439ea6110f9136c0a244 |
| institution | Kabale University |
| issn | 2699-9293 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Photonics Research |
| spelling | doaj-art-852819130eca439ea6110f9136c0a2442025-01-06T02:15:53ZengWiley-VCHAdvanced Photonics Research2699-92932025-01-0161n/an/a10.1002/adpr.202400078Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion MechanismGe Wang0Lizhen Chen1Qiuqiang Zhan2Guangyou Fang3Yisen Wang4GBA Branch of Aerospace Information Research Institute Chinese Academy of Sciences Guangdong Provincial Key Laboratory of Terahertz Quantum Electromagnetics Guangzhou 510700 ChinaGBA Branch of Aerospace Information Research Institute Chinese Academy of Sciences Guangdong Provincial Key Laboratory of Terahertz Quantum Electromagnetics Guangzhou 510700 ChinaCentre for Optical and Electromagnetic Research South China Academy of Advanced Optoelectronics South China Normal University Guangzhou 510006 ChinaGBA Branch of Aerospace Information Research Institute Chinese Academy of Sciences Guangdong Provincial Key Laboratory of Terahertz Quantum Electromagnetics Guangzhou 510700 ChinaGBA Branch of Aerospace Information Research Institute Chinese Academy of Sciences Guangdong Provincial Key Laboratory of Terahertz Quantum Electromagnetics Guangzhou 510700 ChinaHerein, a novel approach is presented to mitigate the fluorescence interference during the detection of vibrational signal via the stimulated emission depletion (STED). STED is the mechanism commonly employed in optical imaging; however, its application should not be confined solely to this field. To explore additional possibilities, a novel application of STED in vibrational spectroscopy detection is introduced. Vibrational spectroscopy is a widely used technique for the material detection and identification, but its sensitivity is influenced by impurity signals, especially the fluorescence. The proposed method is capable of suppressing fluorescence without influencing vibrational signal. At the low concentration of fluorescent impurities, the signal‐to‐background ratio of vibrational spectroscopy is 2.6 times as high as that without this method. The introduction of depletion light can enhance the detection of vibrational signals, resulting in more optimal signal detection. A promising new application of STED other than super‐resolution imaging is investigated.https://doi.org/10.1002/adpr.202400078fluorescencessignal‐to‐background ratiosstimulated emission depletionsvibrational spectroscopies |
| spellingShingle | Ge Wang Lizhen Chen Qiuqiang Zhan Guangyou Fang Yisen Wang Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion Mechanism Advanced Photonics Research fluorescences signal‐to‐background ratios stimulated emission depletions vibrational spectroscopies |
| title | Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion Mechanism |
| title_full | Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion Mechanism |
| title_fullStr | Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion Mechanism |
| title_full_unstemmed | Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion Mechanism |
| title_short | Enhancement of Signal‐to‐Background Ratio in Molecular Vibrational Signal Extraction by Stimulated Emission Depletion Mechanism |
| title_sort | enhancement of signal to background ratio in molecular vibrational signal extraction by stimulated emission depletion mechanism |
| topic | fluorescences signal‐to‐background ratios stimulated emission depletions vibrational spectroscopies |
| url | https://doi.org/10.1002/adpr.202400078 |
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