Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging
Pseudomonas fragi is a predominant meat-borne spoilage bacterium that is sensitive to CO2 under high-oxygen modified atmosphere packaging (HiOx-MAP). This study was designed to reveal the spoilage potential of a popular wild-type P. fragi T1 in HiOx-MAP beef by whole genome sequencing, and explore t...
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Tsinghua University Press
2024-12-01
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| Series: | Food Science of Animal Products |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSAP.2024.9240084 |
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| author | Jun Yang Yikun Xu Yimin Zhang Rongrong Liang Lixian Zhu Yanwei Mao Xin Luo Xiaoyin Yang |
| author_facet | Jun Yang Yikun Xu Yimin Zhang Rongrong Liang Lixian Zhu Yanwei Mao Xin Luo Xiaoyin Yang |
| author_sort | Jun Yang |
| collection | DOAJ |
| description | Pseudomonas fragi is a predominant meat-borne spoilage bacterium that is sensitive to CO2 under high-oxygen modified atmosphere packaging (HiOx-MAP). This study was designed to reveal the spoilage potential of a popular wild-type P. fragi T1 in HiOx-MAP beef by whole genome sequencing, and explore the bacterial metabolic response to CO2 utilizing combined metabolomic and volatile organic compounds (VOCs) analysis, under treatment (CO2-enriched) HiOx-MAP (TMAP, 50% O2/40% CO2/10% N2) or control (non-CO2) HiOx-MAP (CMAP, 50% O2/50% N2) during chilled storage. Results showed that the strain P. fragi T1 was endued with spoilage-related genes associated with protease, lipase and esterase production, amino acid metabolism, carbon metabolism, sulfur metabolism, and putrescine metabolism, which was responsible for the hydrolysis of meat protein and lipid, as well as off-odor formation. The growth of P. fragi under CMAP resulted in the production of VOCs, such as diacetyl, 1-undecene, 2-undecanone, nonanal, (Z)-5-decen-1-ol, and (E)-2-octenal, etc. The TMAP declined above VOCs concentrations significantly (P < 0.05) by inhibiting P. fragi growth and regulating its metabolic activities. The metabolomic analysis further manifested that CO2 inhibited the P. fragi growth by decreasing cell membrane fluidity, disturbing energy metabolism, and inhibiting amino acid metabolism and nucleotide biosynthesis. This work provides valuable information for understanding the P. fragi-induced meat spoilage phenomena, and the antibacterial mechanism of CO2 against P. fragi. |
| format | Article |
| id | doaj-art-8527ca35d26247279f7667e5dba59c34 |
| institution | Kabale University |
| issn | 2958-4124 2958-3780 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science of Animal Products |
| spelling | doaj-art-8527ca35d26247279f7667e5dba59c342025-01-10T06:49:39ZengTsinghua University PressFood Science of Animal Products2958-41242958-37802024-12-0124924008410.26599/FSAP.2024.9240084Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packagingJun Yang0Yikun Xu1Yimin Zhang2Rongrong Liang3Lixian Zhu4Yanwei Mao5Xin Luo6Xiaoyin Yang7Laboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaLaboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaLaboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaLaboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaLaboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaLaboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaLaboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaLaboratory of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University,Tai’an 271018, ChinaPseudomonas fragi is a predominant meat-borne spoilage bacterium that is sensitive to CO2 under high-oxygen modified atmosphere packaging (HiOx-MAP). This study was designed to reveal the spoilage potential of a popular wild-type P. fragi T1 in HiOx-MAP beef by whole genome sequencing, and explore the bacterial metabolic response to CO2 utilizing combined metabolomic and volatile organic compounds (VOCs) analysis, under treatment (CO2-enriched) HiOx-MAP (TMAP, 50% O2/40% CO2/10% N2) or control (non-CO2) HiOx-MAP (CMAP, 50% O2/50% N2) during chilled storage. Results showed that the strain P. fragi T1 was endued with spoilage-related genes associated with protease, lipase and esterase production, amino acid metabolism, carbon metabolism, sulfur metabolism, and putrescine metabolism, which was responsible for the hydrolysis of meat protein and lipid, as well as off-odor formation. The growth of P. fragi under CMAP resulted in the production of VOCs, such as diacetyl, 1-undecene, 2-undecanone, nonanal, (Z)-5-decen-1-ol, and (E)-2-octenal, etc. The TMAP declined above VOCs concentrations significantly (P < 0.05) by inhibiting P. fragi growth and regulating its metabolic activities. The metabolomic analysis further manifested that CO2 inhibited the P. fragi growth by decreasing cell membrane fluidity, disturbing energy metabolism, and inhibiting amino acid metabolism and nucleotide biosynthesis. This work provides valuable information for understanding the P. fragi-induced meat spoilage phenomena, and the antibacterial mechanism of CO2 against P. fragi.https://www.sciopen.com/article/10.26599/FSAP.2024.9240084pseudomonas fragico2 antibacterial mechanismmetabolomicswhole genome sequencevolatile organic compounds |
| spellingShingle | Jun Yang Yikun Xu Yimin Zhang Rongrong Liang Lixian Zhu Yanwei Mao Xin Luo Xiaoyin Yang Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging Food Science of Animal Products pseudomonas fragi co2 antibacterial mechanism metabolomics whole genome sequence volatile organic compounds |
| title | Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging |
| title_full | Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging |
| title_fullStr | Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging |
| title_full_unstemmed | Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging |
| title_short | Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging |
| title_sort | genome sequence and metabolic analysis of pseudomonas fragi unveil the meat spoilage and co2 antibacterial mechanism under high oxygen modified atmosphere packaging |
| topic | pseudomonas fragi co2 antibacterial mechanism metabolomics whole genome sequence volatile organic compounds |
| url | https://www.sciopen.com/article/10.26599/FSAP.2024.9240084 |
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