Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes

Seed-based restoration methods are increasingly recognized as a relevant tool contributing to halt and reverse the loss of seagrass meadows while providing genetic and evolutionary benefit for the conservation of these habitats. Ad-hoc protocols aimed at maximizing the survival of plantlets obtained...

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Main Authors: Adriana Alagna, Vincenzo Maximiliano Giacalone, Arturo Zenone, Marco Martinez, Giovanni D’Anna, Gaspare Buffa, Caterina Jessica Cavalca, Anna Poli, Giovanna Cristina Varese, Valeria Paola Prigione, Fabio Badalamenti
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1433358/full
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author Adriana Alagna
Adriana Alagna
Vincenzo Maximiliano Giacalone
Vincenzo Maximiliano Giacalone
Arturo Zenone
Arturo Zenone
Arturo Zenone
Marco Martinez
Giovanni D’Anna
Giovanni D’Anna
Gaspare Buffa
Gaspare Buffa
Caterina Jessica Cavalca
Anna Poli
Anna Poli
Giovanna Cristina Varese
Giovanna Cristina Varese
Valeria Paola Prigione
Valeria Paola Prigione
Fabio Badalamenti
Fabio Badalamenti
author_facet Adriana Alagna
Adriana Alagna
Vincenzo Maximiliano Giacalone
Vincenzo Maximiliano Giacalone
Arturo Zenone
Arturo Zenone
Arturo Zenone
Marco Martinez
Giovanni D’Anna
Giovanni D’Anna
Gaspare Buffa
Gaspare Buffa
Caterina Jessica Cavalca
Anna Poli
Anna Poli
Giovanna Cristina Varese
Giovanna Cristina Varese
Valeria Paola Prigione
Valeria Paola Prigione
Fabio Badalamenti
Fabio Badalamenti
author_sort Adriana Alagna
collection DOAJ
description Seed-based restoration methods are increasingly recognized as a relevant tool contributing to halt and reverse the loss of seagrass meadows while providing genetic and evolutionary benefit for the conservation of these habitats. Ad-hoc protocols aimed at maximizing the survival of plantlets obtained from seeds in cultivation systems are therefore required. Previous trials of seedling culture of Posidonia oceanica, the dominant seagrass of the Mediterranean Sea, recorded up to 40% loss due to mould development. In this study we aim to (i) identify the putative causal agents of seed decay and (ii) test the efficacy of copper sulphate (0.2 and 2 ppm) and of tannin-based products derived from chestnut, tara and quebracho in reducing seed and seedling decay, while assessing possible phytotoxic effects on plant development. Halophytophthora lusitanica, H. thermoambigua and a putative new Halophytophtora species were identified as possible causal agents of seed loss. The antimicrobial agents (copper and tannins) reduced seed contamination by 20%, although copper sulphate at 2 ppm strongly inhibited the root growth. Among tannins, chestnut and tara reduced seeds germination by up to 75% and decreased shoot and root development, while quebracho showed a less severe phytotoxic effect. The use of copper sulphate at 0.2 ppm is therefore recommended to prevent P. oceanica seedling loss in culture facilities since it reduces seed contamination with no phytotoxic effects. Our results contribute to improving the seedling culture of one the key species of the Mediterranean Sea, increasing propagule availability for restoration purposes.
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spelling doaj-art-84dca2efd29c45dab55ba81a251aa1dd2024-11-25T04:26:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14333581433358Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposesAdriana Alagna0Adriana Alagna1Vincenzo Maximiliano Giacalone2Vincenzo Maximiliano Giacalone3Arturo Zenone4Arturo Zenone5Arturo Zenone6Marco Martinez7Giovanni D’Anna8Giovanni D’Anna9Gaspare Buffa10Gaspare Buffa11Caterina Jessica Cavalca12Anna Poli13Anna Poli14Giovanna Cristina Varese15Giovanna Cristina Varese16Valeria Paola Prigione17Valeria Paola Prigione18Fabio Badalamenti19Fabio Badalamenti20Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Palermo, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyInstitute for the Anthropic impacts and Sustainability in Marine Environment, IAS-CNR, Capo Granitola, ItalyDepartment of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Palermo, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyInstitute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Palermo, ItalyInstitute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Palermo, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyInstitute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Castellammare del Golfo, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyInstitute for the Anthropic impacts and Sustainability in Marine Environment, IAS-CNR, Capo Granitola, ItalyInstitute for the Anthropic impacts and Sustainability in Marine Environment, IAS-CNR, Capo Granitola, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Mycotheca Universitatis Taurinensis (MUT), Torino, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Mycotheca Universitatis Taurinensis (MUT), Torino, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Mycotheca Universitatis Taurinensis (MUT), Torino, ItalyNational Biodiversity Future Centre (NBFC), Palermo, ItalyInstitute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Palermo, ItalySeed-based restoration methods are increasingly recognized as a relevant tool contributing to halt and reverse the loss of seagrass meadows while providing genetic and evolutionary benefit for the conservation of these habitats. Ad-hoc protocols aimed at maximizing the survival of plantlets obtained from seeds in cultivation systems are therefore required. Previous trials of seedling culture of Posidonia oceanica, the dominant seagrass of the Mediterranean Sea, recorded up to 40% loss due to mould development. In this study we aim to (i) identify the putative causal agents of seed decay and (ii) test the efficacy of copper sulphate (0.2 and 2 ppm) and of tannin-based products derived from chestnut, tara and quebracho in reducing seed and seedling decay, while assessing possible phytotoxic effects on plant development. Halophytophthora lusitanica, H. thermoambigua and a putative new Halophytophtora species were identified as possible causal agents of seed loss. The antimicrobial agents (copper and tannins) reduced seed contamination by 20%, although copper sulphate at 2 ppm strongly inhibited the root growth. Among tannins, chestnut and tara reduced seeds germination by up to 75% and decreased shoot and root development, while quebracho showed a less severe phytotoxic effect. The use of copper sulphate at 0.2 ppm is therefore recommended to prevent P. oceanica seedling loss in culture facilities since it reduces seed contamination with no phytotoxic effects. Our results contribute to improving the seedling culture of one the key species of the Mediterranean Sea, increasing propagule availability for restoration purposes.https://www.frontiersin.org/articles/10.3389/fpls.2024.1433358/fullPosidonia oceanicaseed cultivationtanninscopper sulphateseagrass propagationpathogen
spellingShingle Adriana Alagna
Adriana Alagna
Vincenzo Maximiliano Giacalone
Vincenzo Maximiliano Giacalone
Arturo Zenone
Arturo Zenone
Arturo Zenone
Marco Martinez
Giovanni D’Anna
Giovanni D’Anna
Gaspare Buffa
Gaspare Buffa
Caterina Jessica Cavalca
Anna Poli
Anna Poli
Giovanna Cristina Varese
Giovanna Cristina Varese
Valeria Paola Prigione
Valeria Paola Prigione
Fabio Badalamenti
Fabio Badalamenti
Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes
Frontiers in Plant Science
Posidonia oceanica
seed cultivation
tannins
copper sulphate
seagrass propagation
pathogen
title Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes
title_full Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes
title_fullStr Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes
title_full_unstemmed Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes
title_short Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes
title_sort tannins and copper sulphate as antimicrobial agents to prevent contamination of posidonia oceanica seedling culture for restoration purposes
topic Posidonia oceanica
seed cultivation
tannins
copper sulphate
seagrass propagation
pathogen
url https://www.frontiersin.org/articles/10.3389/fpls.2024.1433358/full
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