<i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints
The highly valued oil of <i>Rosa damascena</i> Mill. (Rosaceae), widely used in high perfumery, cosmetics, and other spheres of human life, obliges us to know and study the safety profile of the product obtained from the water–steam distillation of fresh rose petals. The genotoxicity of...
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2024-12-01
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| author | Tsvetelina Gerasimova Svetla Gateva Gabriele Jovtchev Tsveta Angelova Margarita Topashka-Ancheva Ana Dobreva Milka Mileva |
| author_facet | Tsvetelina Gerasimova Svetla Gateva Gabriele Jovtchev Tsveta Angelova Margarita Topashka-Ancheva Ana Dobreva Milka Mileva |
| author_sort | Tsvetelina Gerasimova |
| collection | DOAJ |
| description | The highly valued oil of <i>Rosa damascena</i> Mill. (Rosaceae), widely used in high perfumery, cosmetics, and other spheres of human life, obliges us to know and study the safety profile of the product obtained from the water–steam distillation of fresh rose petals. The genotoxicity of the essential oil (EsO) has not been thoroughly studied despite its wide range of applications. That predetermined the object of this study—to evaluate, through classical cytogenetic methods, the possible cytotoxic/genotoxic activities of <i>R. damascena</i> Mill. EsO (EsORdm) in three different test systems: plant root meristem cells, mammalian bone marrow cells, and human lymphocyte cultures. The rose essential oil showed varying concentration- and time-dependent cytotoxic and genotoxic effects depending on the test system used, and it was established that the oil showed moderate cytotoxicity in lymphocyte cultures and non-high cytotoxicity in ICR mice but none in barley. Both barley and human lymphocytes showed a genotoxic effect with a dose-dependent increase in chromosomal aberrations (CAs) and a substantial rise in micronucleus (MN) frequency, while no genotoxicity was observed in bone marrow cells at the applied concentrations. Human lymphocytes exhibited the highest susceptibility to cytotoxic and genotoxic actions of the EsO. As a valuable plant-derived aromatic product with versatile uses in human life, <i>R. damascena</i> Mill. essential oil should be used in an appropriate concentration range tailored to cellular sensitivity. |
| format | Article |
| id | doaj-art-84bed796393f49979c3017a3b8053e25 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-84bed796393f49979c3017a3b8053e252025-01-10T13:18:48ZengMDPI AGMolecules1420-30492024-12-013017810.3390/molecules30010078<i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic EndpointsTsvetelina Gerasimova0Svetla Gateva1Gabriele Jovtchev2Tsveta Angelova3Margarita Topashka-Ancheva4Ana Dobreva5Milka Mileva6Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Str., 1113 Sofia, BulgariaInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Str., 1113 Sofia, BulgariaInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Str., 1113 Sofia, BulgariaInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Str., 1113 Sofia, BulgariaInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Str., 1113 Sofia, BulgariaInstitute for Roses and Aromatic Plants, Agricultural Academy, 49 Osvobojdenie Blvd., 6100 Kazanlak, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev Str., 1113 Sofia, BulgariaThe highly valued oil of <i>Rosa damascena</i> Mill. (Rosaceae), widely used in high perfumery, cosmetics, and other spheres of human life, obliges us to know and study the safety profile of the product obtained from the water–steam distillation of fresh rose petals. The genotoxicity of the essential oil (EsO) has not been thoroughly studied despite its wide range of applications. That predetermined the object of this study—to evaluate, through classical cytogenetic methods, the possible cytotoxic/genotoxic activities of <i>R. damascena</i> Mill. EsO (EsORdm) in three different test systems: plant root meristem cells, mammalian bone marrow cells, and human lymphocyte cultures. The rose essential oil showed varying concentration- and time-dependent cytotoxic and genotoxic effects depending on the test system used, and it was established that the oil showed moderate cytotoxicity in lymphocyte cultures and non-high cytotoxicity in ICR mice but none in barley. Both barley and human lymphocytes showed a genotoxic effect with a dose-dependent increase in chromosomal aberrations (CAs) and a substantial rise in micronucleus (MN) frequency, while no genotoxicity was observed in bone marrow cells at the applied concentrations. Human lymphocytes exhibited the highest susceptibility to cytotoxic and genotoxic actions of the EsO. As a valuable plant-derived aromatic product with versatile uses in human life, <i>R. damascena</i> Mill. essential oil should be used in an appropriate concentration range tailored to cellular sensitivity.https://www.mdpi.com/1420-3049/30/1/78<i>Rosa damascena</i> Mill.essential oilgenotoxicitycytotoxicitychromosomal aberrationsmicronuclei |
| spellingShingle | Tsvetelina Gerasimova Svetla Gateva Gabriele Jovtchev Tsveta Angelova Margarita Topashka-Ancheva Ana Dobreva Milka Mileva <i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints Molecules <i>Rosa damascena</i> Mill. essential oil genotoxicity cytotoxicity chromosomal aberrations micronuclei |
| title | <i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints |
| title_full | <i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints |
| title_fullStr | <i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints |
| title_full_unstemmed | <i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints |
| title_short | <i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints |
| title_sort | i rosa damascena i mill essential oil analysis of in vitro and in vivo genotoxic and cytotoxic potentials by employing three cytogenetic endpoints |
| topic | <i>Rosa damascena</i> Mill. essential oil genotoxicity cytotoxicity chromosomal aberrations micronuclei |
| url | https://www.mdpi.com/1420-3049/30/1/78 |
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