Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats
ObjectiveTo explore the effect of slit guidance ligand 3 （Slit3） on rats with diabetic kidney disease （DKD）-induced fibrosis.MethodsA DKD rat model was established by multiple low-dose injections of streptozotocin combined with high-sugar high-fat diet. Forty Sprague-Dawley （SD） rats were randomly d...
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Editorial Department of Journal of Clinical Nephrology
2025-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| Online Access: | http://www.lcszb.com/article/doi/10.3969/j.issn.1671-2390.2025.01.008 |
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| author | Huang Ming-fang Dong Yang Shadek-jiang Harinul Gan Xin-yu Shi Wen-li Lu Chen |
| author_facet | Huang Ming-fang Dong Yang Shadek-jiang Harinul Gan Xin-yu Shi Wen-li Lu Chen |
| author_sort | Huang Ming-fang |
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| description | ObjectiveTo explore the effect of slit guidance ligand 3 （Slit3） on rats with diabetic kidney disease （DKD）-induced fibrosis.MethodsA DKD rat model was established by multiple low-dose injections of streptozotocin combined with high-sugar high-fat diet. Forty Sprague-Dawley （SD） rats were randomly divided into the blank control group （control）， diabetes mellitus （DM） group， 4-week DKD group and 8-week DKD group， with 10 rats in each group by random number table method. After successful modeling， there were 8 rats in the control group， and 6 in each of the remaining groups. The 24 h urinary protein content was detected. Total cholesterol （TC）， triglyceride （TG）， blood urea nitrogen （BUN）， serum creatinine （Scr）， and albumin （Alb） were analyzed after abdominal aorta blood collection. Hematoxylin and eosin （H&E） staining was used to observe the histopathological changes of the kidney. Kidney fibrosis degree was examined by Masson’s trichrome staining. The reverse-transcription quantitative real-time polymerase chain reaction （RT-qPCR） was performed to detect mRNA levels of Slit3， α-smooth muscle actin （α-SMA）， and transforming growth factor-β1 （TGF-β1） in kidney tissues， and their protein levels were detected by Western blot. The expression and localization of Slit3 in kidney tissues were detected by immunohistochemical staining.ResultsCompared with the control group， 24-hour urinary protein content ［（37.18 ± 5.55）mg、（47.38 ± 18.19）mg vs （10.66 ± 4.87）mg］， BUN ［（19.18 ± 7.97）mmol/L、（21.01 ± 6.74）mmol/L vs （6.86 ± 1.15）mmol/L］ and Scr ［（58.02 ± 12.49）μmol/L、（61.18 ± 20.76）μmol/L vs （28.34 ± 3.35）μmol/L］ of 4-week and 8-week DKD groups were significantly higher （P<0.05）. In comparison to the control group， TC ［（3.75 ± 3.21）mmol/L、（2.44 ± 2.08）mmol/L vs （1.37 ± 0.32）mmol/L］ was significantly higher， while TG ［（0.46 ± 0.64）mmol/L、（0.32 ± 0.50）mmol/L vs （0.93 ± 0.46）mmol/L］ was significantly lower in the 4-week and 8-week DKD groups （P<0.05）. Compared with the control group， the protein levels of Slit3 ［（0.100 ± 0.002）、（0.120 ± 0.003） vs （0.050 ± 0.006）］， α-SMA ［（0.370 ± 0.011）、（0.590 ± 0.003） vs （0.070 ± 0.006）］ and TGF-β1 ［（0.810 ± 0.024）、（0.940 ± 0.01） vs （0.520 ± 0.028）］ in rat kidney tissue were significantly higher in the 4-week and 8-week DKD groups （P<0.05）； so as their mRNA levels ［（2.520 ± 0.164）、（3.016 ± 0.226） vs （1.020 ± 0.034）； （3.010 ± 0.160）、（3.560 ± 0.218） vs （1.000 ± 0.100）； （3.030 ± 0.185）、（3.550 ± 0.194） vs （1.010 ± 0.087）］. Compared with the DM group， the protein levels of Slit3 ［（0.100 ± 0.002）、（0.120 ± 0.003） vs （0.070 ± 0.004）］， α-SMA ［（0.370 ± 0.011）、（0.590 ± 0.003） vs （0.220 ± 0.014）］ and TGF-β1 ［（0.810 ± 0.024）、（0.940 ± 0.010） vs （0.700 ± 0.028）］ in rat kidney tissue were significantly higher in the 4-week and 8-week DKD groups （P<0.05）， especially in the 8-week DKD group （P<0.01）. No significant differences in the mRNA levels of Slit3， α-SMA and TGF-β1 were examined in the rat kidney tissue between 4-week and 8-week DKD groups （P>0.05）.ConclusionSlit3 can significantly aggravate kidney fibrosis in DKD rats. |
| format | Article |
| id | doaj-art-84a645eef25d47bb915d011edf086fb7 |
| institution | Kabale University |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-84a645eef25d47bb915d011edf086fb72025-01-17T08:29:52ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902025-01-01251505810.3969/j.issn.1671-2390.2025.01.0081671-2390（2025）01-0050-09Effect of Slit3 on kidney fibrosis in diabetic kidney disease ratsHuang Ming-fang0Dong Yang1Shadek-jiang Harinul2Gan Xin-yu3Shi Wen-li4Lu Chen5Kidney Disease Center，the First Affiliated Hospital of Xinjiang Medical University，Urumqi 830054，ChinaKidney Disease Center，the First Affiliated Hospital of Xinjiang Medical University，Urumqi 830054，ChinaKidney Disease Center，the First Affiliated Hospital of Xinjiang Medical University，Urumqi 830054，ChinaKidney Disease Center，the First Affiliated Hospital of Xinjiang Medical University，Urumqi 830054，ChinaDepartment of Nephrology，People's Hospital of Xinjiang Uygur Autonomous Region，Urumqi 830001，ChinaKidney Disease Center，the First Affiliated Hospital of Xinjiang Medical University，Urumqi 830054，ChinaObjectiveTo explore the effect of slit guidance ligand 3 （Slit3） on rats with diabetic kidney disease （DKD）-induced fibrosis.MethodsA DKD rat model was established by multiple low-dose injections of streptozotocin combined with high-sugar high-fat diet. Forty Sprague-Dawley （SD） rats were randomly divided into the blank control group （control）， diabetes mellitus （DM） group， 4-week DKD group and 8-week DKD group， with 10 rats in each group by random number table method. After successful modeling， there were 8 rats in the control group， and 6 in each of the remaining groups. The 24 h urinary protein content was detected. Total cholesterol （TC）， triglyceride （TG）， blood urea nitrogen （BUN）， serum creatinine （Scr）， and albumin （Alb） were analyzed after abdominal aorta blood collection. Hematoxylin and eosin （H&E） staining was used to observe the histopathological changes of the kidney. Kidney fibrosis degree was examined by Masson’s trichrome staining. The reverse-transcription quantitative real-time polymerase chain reaction （RT-qPCR） was performed to detect mRNA levels of Slit3， α-smooth muscle actin （α-SMA）， and transforming growth factor-β1 （TGF-β1） in kidney tissues， and their protein levels were detected by Western blot. The expression and localization of Slit3 in kidney tissues were detected by immunohistochemical staining.ResultsCompared with the control group， 24-hour urinary protein content ［（37.18 ± 5.55）mg、（47.38 ± 18.19）mg vs （10.66 ± 4.87）mg］， BUN ［（19.18 ± 7.97）mmol/L、（21.01 ± 6.74）mmol/L vs （6.86 ± 1.15）mmol/L］ and Scr ［（58.02 ± 12.49）μmol/L、（61.18 ± 20.76）μmol/L vs （28.34 ± 3.35）μmol/L］ of 4-week and 8-week DKD groups were significantly higher （P<0.05）. In comparison to the control group， TC ［（3.75 ± 3.21）mmol/L、（2.44 ± 2.08）mmol/L vs （1.37 ± 0.32）mmol/L］ was significantly higher， while TG ［（0.46 ± 0.64）mmol/L、（0.32 ± 0.50）mmol/L vs （0.93 ± 0.46）mmol/L］ was significantly lower in the 4-week and 8-week DKD groups （P<0.05）. Compared with the control group， the protein levels of Slit3 ［（0.100 ± 0.002）、（0.120 ± 0.003） vs （0.050 ± 0.006）］， α-SMA ［（0.370 ± 0.011）、（0.590 ± 0.003） vs （0.070 ± 0.006）］ and TGF-β1 ［（0.810 ± 0.024）、（0.940 ± 0.01） vs （0.520 ± 0.028）］ in rat kidney tissue were significantly higher in the 4-week and 8-week DKD groups （P<0.05）； so as their mRNA levels ［（2.520 ± 0.164）、（3.016 ± 0.226） vs （1.020 ± 0.034）； （3.010 ± 0.160）、（3.560 ± 0.218） vs （1.000 ± 0.100）； （3.030 ± 0.185）、（3.550 ± 0.194） vs （1.010 ± 0.087）］. Compared with the DM group， the protein levels of Slit3 ［（0.100 ± 0.002）、（0.120 ± 0.003） vs （0.070 ± 0.004）］， α-SMA ［（0.370 ± 0.011）、（0.590 ± 0.003） vs （0.220 ± 0.014）］ and TGF-β1 ［（0.810 ± 0.024）、（0.940 ± 0.010） vs （0.700 ± 0.028）］ in rat kidney tissue were significantly higher in the 4-week and 8-week DKD groups （P<0.05）， especially in the 8-week DKD group （P<0.01）. No significant differences in the mRNA levels of Slit3， α-SMA and TGF-β1 were examined in the rat kidney tissue between 4-week and 8-week DKD groups （P>0.05）.ConclusionSlit3 can significantly aggravate kidney fibrosis in DKD rats.http://www.lcszb.com/article/doi/10.3969/j.issn.1671-2390.2025.01.008slit guidance ligand 3diabetic kidney diseasekidney fibrosis |
| spellingShingle | Huang Ming-fang Dong Yang Shadek-jiang Harinul Gan Xin-yu Shi Wen-li Lu Chen Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats Linchuang shenzangbing zazhi slit guidance ligand 3 diabetic kidney disease kidney fibrosis |
| title | Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats |
| title_full | Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats |
| title_fullStr | Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats |
| title_full_unstemmed | Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats |
| title_short | Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats |
| title_sort | effect of slit3 on kidney fibrosis in diabetic kidney disease rats |
| topic | slit guidance ligand 3 diabetic kidney disease kidney fibrosis |
| url | http://www.lcszb.com/article/doi/10.3969/j.issn.1671-2390.2025.01.008 |
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