GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max)
Brassinosteroids (BRs) are key phytohormones influencing soybean development, yet their role in symbiosis remains unclear. Here, the RNA-Seq was used to identify important gene associated with BRs and symbiotic nitrogen fixation, and the function of candidate gene was verified by transgenic hairy ro...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1507307/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841527034949402624 |
|---|---|
| author | Mingliang Yang Mingliang Yang Chengjun Lei Chengjun Lei Chao Ma Chao Ma Xiuming Hou Xiuming Hou Mingming Yao Mingming Yao Liang Mi Liang Mi Enliang Liu Linli Xu Shukun Wang Shukun Wang Chunyan Liu Chunyan Liu Qingshan Chen Qingshan Chen Dawei Xin Dawei Xin Chang Xu Chang Xu Jinhui Wang Jinhui Wang |
| author_facet | Mingliang Yang Mingliang Yang Chengjun Lei Chengjun Lei Chao Ma Chao Ma Xiuming Hou Xiuming Hou Mingming Yao Mingming Yao Liang Mi Liang Mi Enliang Liu Linli Xu Shukun Wang Shukun Wang Chunyan Liu Chunyan Liu Qingshan Chen Qingshan Chen Dawei Xin Dawei Xin Chang Xu Chang Xu Jinhui Wang Jinhui Wang |
| author_sort | Mingliang Yang |
| collection | DOAJ |
| description | Brassinosteroids (BRs) are key phytohormones influencing soybean development, yet their role in symbiosis remains unclear. Here, the RNA-Seq was used to identify important gene associated with BRs and symbiotic nitrogen fixation, and the function of candidate gene was verified by transgenic hairy roots. The result shows that the RNA-Seq analysis was conducted in which BR signaling was found to suppress nodule formation and many DEGs enriched in immunity-related pathways. WGCNA analyses led to the identification of GmWRKY33a as being responsive to BR signaling in the context of symbiosis establishment. Transgenic hairy roots analyses indicated that GmWRKY33a served as a negative regulator of the establishment of symbiosis. The qRT-PCR analysis confirmed that BR signaling upregulates GmWRKY33a, leading to nodulation suppression and activation of soybean immune responses. In summary, our research revealed that BR suppresses root nodule formation by modulating the immune signaling pathway in soybean roots. We further identified that GmWRKY33a, a crucial transcription factor in BR signaling, plays a negative role in the symbiotic establishment. |
| format | Article |
| id | doaj-art-844aa1800f8e4069a1167a8bcc953028 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-844aa1800f8e4069a1167a8bcc9530282025-01-16T06:10:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15073071507307GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max)Mingliang Yang0Mingliang Yang1Chengjun Lei2Chengjun Lei3Chao Ma4Chao Ma5Xiuming Hou6Xiuming Hou7Mingming Yao8Mingming Yao9Liang Mi10Liang Mi11Enliang Liu12Linli Xu13Shukun Wang14Shukun Wang15Chunyan Liu16Chunyan Liu17Qingshan Chen18Qingshan Chen19Dawei Xin20Dawei Xin21Chang Xu22Chang Xu23Jinhui Wang24Jinhui Wang25Heilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaGrain Crops Institute, XinJiang Academy of Agricultural Sciences, Urumqi, Xinjiang Uygur, ChinaGrain Crops Institute, XinJiang Academy of Agricultural Sciences, Urumqi, Xinjiang Uygur, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaHeilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin, Heilongjiang, ChinaNational Key Laboratory of Smart Farm Technologies and Systems, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, ChinaBrassinosteroids (BRs) are key phytohormones influencing soybean development, yet their role in symbiosis remains unclear. Here, the RNA-Seq was used to identify important gene associated with BRs and symbiotic nitrogen fixation, and the function of candidate gene was verified by transgenic hairy roots. The result shows that the RNA-Seq analysis was conducted in which BR signaling was found to suppress nodule formation and many DEGs enriched in immunity-related pathways. WGCNA analyses led to the identification of GmWRKY33a as being responsive to BR signaling in the context of symbiosis establishment. Transgenic hairy roots analyses indicated that GmWRKY33a served as a negative regulator of the establishment of symbiosis. The qRT-PCR analysis confirmed that BR signaling upregulates GmWRKY33a, leading to nodulation suppression and activation of soybean immune responses. In summary, our research revealed that BR suppresses root nodule formation by modulating the immune signaling pathway in soybean roots. We further identified that GmWRKY33a, a crucial transcription factor in BR signaling, plays a negative role in the symbiotic establishment.https://www.frontiersin.org/articles/10.3389/fpls.2024.1507307/fullsoybeansymbiosisbrassinosteroids (BRs)RNA-SeqWGCNAGmWRKY33a |
| spellingShingle | Mingliang Yang Mingliang Yang Chengjun Lei Chengjun Lei Chao Ma Chao Ma Xiuming Hou Xiuming Hou Mingming Yao Mingming Yao Liang Mi Liang Mi Enliang Liu Linli Xu Shukun Wang Shukun Wang Chunyan Liu Chunyan Liu Qingshan Chen Qingshan Chen Dawei Xin Dawei Xin Chang Xu Chang Xu Jinhui Wang Jinhui Wang GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) Frontiers in Plant Science soybean symbiosis brassinosteroids (BRs) RNA-Seq WGCNA GmWRKY33a |
| title | GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) |
| title_full | GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) |
| title_fullStr | GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) |
| title_full_unstemmed | GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) |
| title_short | GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) |
| title_sort | gmwrky33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean glycine max |
| topic | soybean symbiosis brassinosteroids (BRs) RNA-Seq WGCNA GmWRKY33a |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1507307/full |
| work_keys_str_mv | AT mingliangyang gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT mingliangyang gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT chengjunlei gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT chengjunlei gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT chaoma gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT chaoma gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT xiuminghou gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT xiuminghou gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT mingmingyao gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT mingmingyao gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT liangmi gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT liangmi gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT enliangliu gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT linlixu gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT shukunwang gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT shukunwang gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT chunyanliu gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT chunyanliu gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT qingshanchen gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT qingshanchen gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT daweixin gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT daweixin gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT changxu gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT changxu gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT jinhuiwang gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax AT jinhuiwang gmwrky33aisahubgeneresponsivetobrassinosteroidsignalingthatsuppressesnodulationinsoybeanglycinemax |