The determination of alpha naphthyl acetate esterase (anae) positivity in peripheral blood leukocytes of kyrgyzstan?s mountain yaks (bos grinniens)
<b>Aim:</b> A lysosomal enzyme, alpha naphthyl acetate esterase (ANAE), has been used to differentiate T and B lymphocytes, and monocytes in various species in peripheral blood smears. In this study, it was aimed to determine the positivity of the ANAE in peripheral blood leukocytes of K...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2020-10-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1295 |
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| Summary: | <b>Aim:</b> A lysosomal enzyme, alpha naphthyl acetate esterase (ANAE),
has been used to differentiate T and B lymphocytes, and monocytes
in various species in peripheral blood smears. In this study, it was
aimed to determine the positivity of the ANAE in peripheral blood
leukocytes of Kyrgyzstan's mountain yaks, as also known Topoz, (Bos
grinniens).<p>
<b>Materials and Methods:</b> As a material, the peripheral blood samples
collected from healthy thirteen Kyrgyzstan's mountain yaks, 2?4
year old aged, were used. ANAE was demonstrated on smears prepared
from blood samples.<p>
<b>Results:</b> ANAE-positivity was observed in all monocytes and only
some lymphocytes. ANAE-positivity pattern in lymphocytes was
dot like characterized with 1-4 reddish-brown cytoplasmic granules
while all monocytes have a diffuse, fine granular enzymatic reaction
along whole cytoplasm. No enzymatic reaction was observed in neutrophil
and eosinophil leukocytes. The ANAE positive lymphocyte
rate was found to be 58,85 % in Kyrgyzstan's mountain yaks.<p>
<b>Conclusion:</b> Studies on Kyrgyzstan mountain yaks have been extremely
limited due to their living conditions and aggressive temperaments
of these animals. Therefore, any kind of structural, histological,
enzyme histochemical, physiological, biochemical and
anatomical studies on these animals, as well as case reports of various
diseases that may be faced by veterinary clinicians or academicians
working in the country, will guide the studies to be carried out
on these animals in future. |
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| ISSN: | 1309-6958 2146-1953 |