The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver Samples
Background: Epidermal growth factor receptor 4 (ERBB4) and neuregulin 4 (NRG4) have been shown to reduce steatosis and prevent the development of non-alcoholic steatohepatitis in mouse models, but little to nothing is known about their role in non-alcoholic fatty liver disease (NA...
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IMR Press
2024-12-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/29/12/10.31083/j.fbl2912414 |
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| author | Maria Bograya Maria Vulf Anastasia Minchenko Natalia Gazatova Georgy Kuznetsov Natalia Todosenko Larisa Volkova Pavel Ivanov Larisa Litvinova |
| author_facet | Maria Bograya Maria Vulf Anastasia Minchenko Natalia Gazatova Georgy Kuznetsov Natalia Todosenko Larisa Volkova Pavel Ivanov Larisa Litvinova |
| author_sort | Maria Bograya |
| collection | DOAJ |
| description | Background: Epidermal growth factor receptor 4 (ERBB4) and neuregulin 4 (NRG4) have been shown to reduce steatosis and prevent the development of non-alcoholic steatohepatitis in mouse models, but little to nothing is known about their role in non-alcoholic fatty liver disease (NAFLD) in humans. This study is the first to investigate the expression of ERBB4 and NRG4 mRNAs and their role in lipid metabolism in the livers of individuals with obesity, type 2 diabetes and biopsy-proven NAFLD. Methods: Liver biospecimens were obtained intraoperatively from 80 individuals. Quantitative reverse transcription polymerase chain reaction was used to measure the expression levels of mRNAs ERBB4 and NRG4, as well as key lipogenesis genes in the liver tissue of the donors. Histological analysis was conducted on liver biopsies from 36 subjects, and the levels of the examined transcripts were compared with the stage of NAFLD. Results: In individuals with elevated body mass index (BMI), ERBB4 and NRG4 levels decreased, while ACACA levels increased. A strong negative correlation was observed between NRG4 and ACACA levels. No deregulation of the analyzed transcripts was detected in NAFLD. Conclusions: The study demonstrates a decrease in ERBB4 and NRG4 mRNA expression in the livers of subjects with high BMI but not in those with NAFLD. The correlation of the studied transcripts with major lipogenesis genes was assessed, and on this basis a putative scheme for NRG4-mediated suppression of hepatic de novo lipogenesis was hypothesised, offering new research vectors in this field. |
| format | Article |
| id | doaj-art-843c910104e646e29356d4508d6ea3de |
| institution | Kabale University |
| issn | 2768-6701 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj-art-843c910104e646e29356d4508d6ea3de2024-12-30T11:39:30ZengIMR PressFrontiers in Bioscience-Landmark2768-67012024-12-01291241410.31083/j.fbl2912414S2768-6701(24)01547-8The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver SamplesMaria Bograya0Maria Vulf1Anastasia Minchenko2Natalia Gazatova3Georgy Kuznetsov4Natalia Todosenko5Larisa Volkova6Pavel Ivanov7Larisa Litvinova8Center for Immunology and Cellular Biotechnology, Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University, 236001 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University, 236001 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University, 236001 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University, 236001 Kaliningrad, RussiaSurgical Department, Regional Clinical Hospital Kaliningrad, 236035 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University, 236001 Kaliningrad, RussiaMedical Faculty, Moscow University for Industry and Finance “Synergy”, 125190 Moscow, RussiaCenter for Immunology and Cellular Biotechnology, Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University, 236001 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University, 236001 Kaliningrad, RussiaBackground: Epidermal growth factor receptor 4 (ERBB4) and neuregulin 4 (NRG4) have been shown to reduce steatosis and prevent the development of non-alcoholic steatohepatitis in mouse models, but little to nothing is known about their role in non-alcoholic fatty liver disease (NAFLD) in humans. This study is the first to investigate the expression of ERBB4 and NRG4 mRNAs and their role in lipid metabolism in the livers of individuals with obesity, type 2 diabetes and biopsy-proven NAFLD. Methods: Liver biospecimens were obtained intraoperatively from 80 individuals. Quantitative reverse transcription polymerase chain reaction was used to measure the expression levels of mRNAs ERBB4 and NRG4, as well as key lipogenesis genes in the liver tissue of the donors. Histological analysis was conducted on liver biopsies from 36 subjects, and the levels of the examined transcripts were compared with the stage of NAFLD. Results: In individuals with elevated body mass index (BMI), ERBB4 and NRG4 levels decreased, while ACACA levels increased. A strong negative correlation was observed between NRG4 and ACACA levels. No deregulation of the analyzed transcripts was detected in NAFLD. Conclusions: The study demonstrates a decrease in ERBB4 and NRG4 mRNA expression in the livers of subjects with high BMI but not in those with NAFLD. The correlation of the studied transcripts with major lipogenesis genes was assessed, and on this basis a putative scheme for NRG4-mediated suppression of hepatic de novo lipogenesis was hypothesised, offering new research vectors in this field.https://www.imrpress.com/journal/FBL/29/12/10.31083/j.fbl2912414erbb4nrg4obesitynafldsteatosisnash |
| spellingShingle | Maria Bograya Maria Vulf Anastasia Minchenko Natalia Gazatova Georgy Kuznetsov Natalia Todosenko Larisa Volkova Pavel Ivanov Larisa Litvinova The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver Samples Frontiers in Bioscience-Landmark erbb4 nrg4 obesity nafld steatosis nash |
| title | The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver Samples |
| title_full | The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver Samples |
| title_fullStr | The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver Samples |
| title_full_unstemmed | The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver Samples |
| title_short | The Putative Antilipogenic Role of NRG4 and ERBB4: First Expression Study on Human Liver Samples |
| title_sort | putative antilipogenic role of nrg4 and erbb4 first expression study on human liver samples |
| topic | erbb4 nrg4 obesity nafld steatosis nash |
| url | https://www.imrpress.com/journal/FBL/29/12/10.31083/j.fbl2912414 |
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