Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99
Abstract This study aimed to evaluate the safety profile and beneficial effects of Heyndrickxia coagulans strain BC99 (BC99) for potential use in functional foods and pharmaceuticals. We began with whole genome sequencing of BC99, followed by a comprehensive safety assessment comprising genome analy...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-78202-y |
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| author | Ying Wu Zhiyi Wu Yinyin Gao Yixuan Fan Yao Dong Yinan Zhang Zhonghui Gai Shaobin Gu |
| author_facet | Ying Wu Zhiyi Wu Yinyin Gao Yixuan Fan Yao Dong Yinan Zhang Zhonghui Gai Shaobin Gu |
| author_sort | Ying Wu |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the safety profile and beneficial effects of Heyndrickxia coagulans strain BC99 (BC99) for potential use in functional foods and pharmaceuticals. We began with whole genome sequencing of BC99, followed by a comprehensive safety assessment comprising genome analysis, hemolysis, cytotoxicity, antibiotic susceptibility tests, and cell adhesion and tolerance studies, along with acute and subacute oral toxicity studies in animal models. BC99 was isolated from a well-characterized collection originating from the feces of a healthy infant. Our results indicated no hemolytic activity on Columbia blood agar plates and broad antibiotic sensitivity, including to gentamicin, ampicillin, chloramphenicol, ciprofloxacin, and others. Cytotoxicity testing confirmed no adverse effects on HT-29 cells and significant adhesive properties to intestinal epithelial cells. Tolerance tests demonstrated over 90% viability of BC99 under simulated gastrointestinal conditions. In vivo studies in mice and rats confirmed the absence of adverse effects following oral administration. Collectively, these findings support BC99’s robust tolerance to gastrointestinal environments, strong adhesion capabilities, and a broad spectrum of antibiotic resistance, underlining its potential as a safe and effective agent for gut microbiota modulation and host health enhancement. |
| format | Article |
| id | doaj-art-83fa86e5ff8847c2bf94e9f7eae416f8 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-83fa86e5ff8847c2bf94e9f7eae416f82024-11-10T12:16:48ZengNature PortfolioScientific Reports2045-23222024-11-0114111510.1038/s41598-024-78202-yComprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99Ying Wu0Zhiyi Wu1Yinyin Gao2Yixuan Fan3Yao Dong4Yinan Zhang5Zhonghui Gai6Shaobin Gu7College of Food and Bioengineering, Henan University of Science and TechnologyDepartment of Research and Development, Henan Animic Biotechnology Co., LtdCollege of Food and Bioengineering, Henan University of Science and TechnologyDepartment of Research and Development, Henan Animic Biotechnology Co., LtdGermline Stem Cells and Microenvironment Lab, College of Animal Science and Technology, Nanjing Agricultural UniversityShanghai Institute of Quality Inspection and Technical ResearchDepartment of Research and Development, Henan Animic Biotechnology Co., LtdCollege of Food and Bioengineering, Henan University of Science and TechnologyAbstract This study aimed to evaluate the safety profile and beneficial effects of Heyndrickxia coagulans strain BC99 (BC99) for potential use in functional foods and pharmaceuticals. We began with whole genome sequencing of BC99, followed by a comprehensive safety assessment comprising genome analysis, hemolysis, cytotoxicity, antibiotic susceptibility tests, and cell adhesion and tolerance studies, along with acute and subacute oral toxicity studies in animal models. BC99 was isolated from a well-characterized collection originating from the feces of a healthy infant. Our results indicated no hemolytic activity on Columbia blood agar plates and broad antibiotic sensitivity, including to gentamicin, ampicillin, chloramphenicol, ciprofloxacin, and others. Cytotoxicity testing confirmed no adverse effects on HT-29 cells and significant adhesive properties to intestinal epithelial cells. Tolerance tests demonstrated over 90% viability of BC99 under simulated gastrointestinal conditions. In vivo studies in mice and rats confirmed the absence of adverse effects following oral administration. Collectively, these findings support BC99’s robust tolerance to gastrointestinal environments, strong adhesion capabilities, and a broad spectrum of antibiotic resistance, underlining its potential as a safe and effective agent for gut microbiota modulation and host health enhancement.https://doi.org/10.1038/s41598-024-78202-yHeyndrickxia coagulansGenomic analysisSafetyIn vitroIn vivo |
| spellingShingle | Ying Wu Zhiyi Wu Yinyin Gao Yixuan Fan Yao Dong Yinan Zhang Zhonghui Gai Shaobin Gu Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99 Scientific Reports Heyndrickxia coagulans Genomic analysis Safety In vitro In vivo |
| title | Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99 |
| title_full | Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99 |
| title_fullStr | Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99 |
| title_full_unstemmed | Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99 |
| title_short | Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99 |
| title_sort | comprehensive genomic analysis and evaluation of in vivo and in vitro safety of heyndrickxia coagulans bc99 |
| topic | Heyndrickxia coagulans Genomic analysis Safety In vitro In vivo |
| url | https://doi.org/10.1038/s41598-024-78202-y |
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