Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilation

Abstract Objectives This study aimed to identify the cyclical “on–off” flow of pulmonary microcirculation during inspiration and expiration by sidestream dark field imaging (SDF) technology in vivo and investigate the effects of volume status and driving pressure on cyclical “on–off” flow of microci...

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Main Authors: Siyi Yuan, Xiangyu Chen, Liangyu Mi, Yi Chi, Haoping Huang, Bo Liu, Chaofu Yue, Zeming Zhao, Longxiang Su, Yun Long, Şakir Akin, Can Ince, Huaiwu He
Format: Article
Language:English
Published: SpringerOpen 2024-12-01
Series:Intensive Care Medicine Experimental
Online Access:https://doi.org/10.1186/s40635-024-00689-6
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author Siyi Yuan
Xiangyu Chen
Liangyu Mi
Yi Chi
Haoping Huang
Bo Liu
Chaofu Yue
Zeming Zhao
Longxiang Su
Yun Long
Şakir Akin
Can Ince
Huaiwu He
author_facet Siyi Yuan
Xiangyu Chen
Liangyu Mi
Yi Chi
Haoping Huang
Bo Liu
Chaofu Yue
Zeming Zhao
Longxiang Su
Yun Long
Şakir Akin
Can Ince
Huaiwu He
author_sort Siyi Yuan
collection DOAJ
description Abstract Objectives This study aimed to identify the cyclical “on–off” flow of pulmonary microcirculation during inspiration and expiration by sidestream dark field imaging (SDF) technology in vivo and investigate the effects of volume status and driving pressure on cyclical “on–off” flow of microcirculation. Methods 24 ARDS-modeled rabbits were randomly divided into high-driving pressure group (HDP group) and low-driving pressure group (LDP group). Lung microcirculation measurements were performed using the SDF microscope at two timepoints (T1 CVP 2–4 mmHg, T2 CVP 8–10 mmHg). From T1 to T2, 10 ml/kg saline was infused to increase CVP. The cyclical “on–off” pulmonary microcirculation was quantitatively assessed by the change of microcirculation between expiration and inspiration. Results Proportion of perfused vessels (PPV), microvascular flow index (MFI), perfused vessel density (PVD), and total vessel density (TVD) at expiration were significantly higher than inspiration in the HDP group. The HDP group has a higher ΔPPV and ΔPVD. After fluid loading, ΔPPV and ΔMFI decreased. TNF-α, IL-6, Ang-2, and vWF levels in the HDP group were higher. The HDP group also has a higher lung wet-weight/body weight ratio, lung wet-to-dry weight ratio, and more severe damage of pulmonary capillaries than the LDP group. Conclusions The difference in alveolar perfused microcirculation between inspiration and expiration defined as cyclical “on–off flow” can be detected. High driving pressure can enhance the cyclical “on–off” flow, and fluid loading can relieve it. High driving pressure can potentially cause injury to pulmonary capillaries due to the phenomenon of “on–off” flow, thereby exacerbating ARDS.
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publisher SpringerOpen
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series Intensive Care Medicine Experimental
spelling doaj-art-83a11f18cea643bbaf92d29dd913ce4a2024-12-08T12:06:57ZengSpringerOpenIntensive Care Medicine Experimental2197-425X2024-12-0112111010.1186/s40635-024-00689-6Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilationSiyi Yuan0Xiangyu Chen1Liangyu Mi2Yi Chi3Haoping Huang4Bo Liu5Chaofu Yue6Zeming Zhao7Longxiang Su8Yun Long9Şakir Akin10Can Ince11Huaiwu He12Department of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceDepartment of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceDepartment of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceDepartment of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceDepartment of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceDepartment of Critical Care Medicine, Affiliated Hospital of Jining Medical UniversityDeparment of Intensive Care Unit, Qu Jing NO.1 HospitalJiamusi Central HospitalDepartment of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceDepartment of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceDepartment of Intensive Care, Erasmus MC University HospitalDepartment of Intensive Care, Erasmus MC University HospitalDepartment of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical ScienceAbstract Objectives This study aimed to identify the cyclical “on–off” flow of pulmonary microcirculation during inspiration and expiration by sidestream dark field imaging (SDF) technology in vivo and investigate the effects of volume status and driving pressure on cyclical “on–off” flow of microcirculation. Methods 24 ARDS-modeled rabbits were randomly divided into high-driving pressure group (HDP group) and low-driving pressure group (LDP group). Lung microcirculation measurements were performed using the SDF microscope at two timepoints (T1 CVP 2–4 mmHg, T2 CVP 8–10 mmHg). From T1 to T2, 10 ml/kg saline was infused to increase CVP. The cyclical “on–off” pulmonary microcirculation was quantitatively assessed by the change of microcirculation between expiration and inspiration. Results Proportion of perfused vessels (PPV), microvascular flow index (MFI), perfused vessel density (PVD), and total vessel density (TVD) at expiration were significantly higher than inspiration in the HDP group. The HDP group has a higher ΔPPV and ΔPVD. After fluid loading, ΔPPV and ΔMFI decreased. TNF-α, IL-6, Ang-2, and vWF levels in the HDP group were higher. The HDP group also has a higher lung wet-weight/body weight ratio, lung wet-to-dry weight ratio, and more severe damage of pulmonary capillaries than the LDP group. Conclusions The difference in alveolar perfused microcirculation between inspiration and expiration defined as cyclical “on–off flow” can be detected. High driving pressure can enhance the cyclical “on–off” flow, and fluid loading can relieve it. High driving pressure can potentially cause injury to pulmonary capillaries due to the phenomenon of “on–off” flow, thereby exacerbating ARDS.https://doi.org/10.1186/s40635-024-00689-6
spellingShingle Siyi Yuan
Xiangyu Chen
Liangyu Mi
Yi Chi
Haoping Huang
Bo Liu
Chaofu Yue
Zeming Zhao
Longxiang Su
Yun Long
Şakir Akin
Can Ince
Huaiwu He
Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilation
Intensive Care Medicine Experimental
title Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilation
title_full Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilation
title_fullStr Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilation
title_full_unstemmed Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilation
title_short Effect of fluid and driving pressure on cyclical “on–off” flow of pulmonary microcirculation during mechanical ventilation
title_sort effect of fluid and driving pressure on cyclical on off flow of pulmonary microcirculation during mechanical ventilation
url https://doi.org/10.1186/s40635-024-00689-6
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