UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuria
Abstract This study evaluated the diagnostic value of the automated UF-5000 parameters and compared it with that of aberrant erythrocytes and acanthocytes classified by microscopy for identifying IgA glomerular hematuria to propose a predictive model for clinical use. It also compared correlations b...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-85575-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544759562207232 |
|---|---|
| author | Shuangshuang Lv Yi Fang Xiaxuan Hu Jian Zhang Xinxin Lou Caihong Chen Lijiao Cao Dongmei Zhang |
| author_facet | Shuangshuang Lv Yi Fang Xiaxuan Hu Jian Zhang Xinxin Lou Caihong Chen Lijiao Cao Dongmei Zhang |
| author_sort | Shuangshuang Lv |
| collection | DOAJ |
| description | Abstract This study evaluated the diagnostic value of the automated UF-5000 parameters and compared it with that of aberrant erythrocytes and acanthocytes classified by microscopy for identifying IgA glomerular hematuria to propose a predictive model for clinical use. It also compared correlations between erythrocyte parameters and malformed erythrocytes. Urine samples from 53 biopsy-proven IgA hematuria cases and 143 non-IGA nephropathic hematuria cases as controls were analyzed. The ratio of small red blood cells to nonlysed red blood cells (UF-sRBC%) and lysed red blood cells (lysed RBCs) showed good diagnostic performance for IgA glomerular hematuria (area under the curve [AUC] = 0.857 [P = 0.000] and AUC = 0.860 [P = 0.000], respectively). Combining UF-sRBC%, lysed RBCs, and urine protein dry chemistry improved the diagnostic accuracy (AUC = 0.967; positive predictive value [PPV] = 91.89%; negative predictive value [NPV] = 93.10%; P = 0.000). This approach surpassed traditional microscopy for aberrant erythrocytes (AUC = 0.895; PPV = 62.27%; NPV = 88.66%; P = 0.008) and acanthocytes (AUC = 0.868; PPV = 72.97%; NPV = 83.65%; P = 0.006). The erythrocyte size index was negatively correlated with the proportion of urinary aberrant erythrocytes (r = − 0.787; P = 0.000). The UF-5000 erythrocyte parameters facilitate rapid identification of IgA nephropathy and could replace manual microscopy. |
| format | Article |
| id | doaj-art-83813b73690e4ceeb6cc4ac8e48fef1d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-83813b73690e4ceeb6cc4ac8e48fef1d2025-01-12T12:19:34ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-85575-1UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuriaShuangshuang Lv0Yi Fang1Xiaxuan Hu2Jian Zhang3Xinxin Lou4Caihong Chen5Lijiao Cao6Dongmei Zhang7Clinical Laboratory, Dongyang People’s HospitalClinical Laboratory, Dongyang People’s HospitalClinical Laboratory, Dongyang People’s HospitalClinical Laboratory, Dongyang People’s HospitalClinical Laboratory, Dongyang People’s HospitalClinical Laboratory, Dongyang People’s HospitalClinical Laboratory, Dongyang People’s HospitalClinical Laboratory, Dongyang People’s HospitalAbstract This study evaluated the diagnostic value of the automated UF-5000 parameters and compared it with that of aberrant erythrocytes and acanthocytes classified by microscopy for identifying IgA glomerular hematuria to propose a predictive model for clinical use. It also compared correlations between erythrocyte parameters and malformed erythrocytes. Urine samples from 53 biopsy-proven IgA hematuria cases and 143 non-IGA nephropathic hematuria cases as controls were analyzed. The ratio of small red blood cells to nonlysed red blood cells (UF-sRBC%) and lysed red blood cells (lysed RBCs) showed good diagnostic performance for IgA glomerular hematuria (area under the curve [AUC] = 0.857 [P = 0.000] and AUC = 0.860 [P = 0.000], respectively). Combining UF-sRBC%, lysed RBCs, and urine protein dry chemistry improved the diagnostic accuracy (AUC = 0.967; positive predictive value [PPV] = 91.89%; negative predictive value [NPV] = 93.10%; P = 0.000). This approach surpassed traditional microscopy for aberrant erythrocytes (AUC = 0.895; PPV = 62.27%; NPV = 88.66%; P = 0.008) and acanthocytes (AUC = 0.868; PPV = 72.97%; NPV = 83.65%; P = 0.006). The erythrocyte size index was negatively correlated with the proportion of urinary aberrant erythrocytes (r = − 0.787; P = 0.000). The UF-5000 erythrocyte parameters facilitate rapid identification of IgA nephropathy and could replace manual microscopy.https://doi.org/10.1038/s41598-025-85575-1HematuriaIgA nephropathyMicroscopyErythrocytesAcanthocytes |
| spellingShingle | Shuangshuang Lv Yi Fang Xiaxuan Hu Jian Zhang Xinxin Lou Caihong Chen Lijiao Cao Dongmei Zhang UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuria Scientific Reports Hematuria IgA nephropathy Microscopy Erythrocytes Acanthocytes |
| title | UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuria |
| title_full | UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuria |
| title_fullStr | UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuria |
| title_full_unstemmed | UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuria |
| title_short | UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA glomerular hematuria |
| title_sort | uf 5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing iga glomerular hematuria |
| topic | Hematuria IgA nephropathy Microscopy Erythrocytes Acanthocytes |
| url | https://doi.org/10.1038/s41598-025-85575-1 |
| work_keys_str_mv | AT shuangshuanglv uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria AT yifang uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria AT xiaxuanhu uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria AT jianzhang uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria AT xinxinlou uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria AT caihongchen uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria AT lijiaocao uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria AT dongmeizhang uf5000urinaryerythrocyteparametersversusurinaryaberranterythrocytesandacanthocytesfordiagnosingigaglomerularhematuria |