Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic Stress
The Golden2-like (GLK) transcription factors belong to the GARP family of transcription factors and play significant roles in plant growth, development, and responses to both abiotic and biotic stresses. This study employed bioinformatics and expression analyses to investigate the regulatory roles o...
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2024-12-01
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| author | Junhui Xiang Pingu Liu Daniel Bimpong Jiayi Shen Xusi Liu Siting Wang Yan Li Youning Wang Dongfang Ma |
| author_facet | Junhui Xiang Pingu Liu Daniel Bimpong Jiayi Shen Xusi Liu Siting Wang Yan Li Youning Wang Dongfang Ma |
| author_sort | Junhui Xiang |
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| description | The Golden2-like (GLK) transcription factors belong to the GARP family of transcription factors and play significant roles in plant growth, development, and responses to both abiotic and biotic stresses. This study employed bioinformatics and expression analyses to investigate the regulatory roles of wheat GLK proteins under various stress conditions, including abscisic acid (ABA) treatment, osmotic stress, and infection by <i>Fusarium graminearum</i>. The study identified 125 <i>TaGLK</i> proteins and revealed that <i>TaGLKs</i> play a significant role in wheat’s development and response to adverse environmental conditions. The results indicate that <i>TaGLKs</i> may serve as potential transcriptional regulators capable of integrating multiple cellular signals to coordinate various developmental and physiological processes. Evolutionary analysis classified the <i>TaGLK</i> proteins into six subgroups, which shared similar conserved domains and motifs. Protein–protein interaction network analysis revealed that <i>TaGLKs</i> are involved in photoreceptor activity, cell cycle progression, and protein regulation. Gene expression analysis of <i>TaGLKs</i> discovered that they play key functions in wheat development, as well as regulation of biotic and abiotic stress conditions. RT-qPCR analysis showed that <i>TaGLKs</i> regulate earlier and late effects of osmotic stress, <i>F. graminearum</i> infections, and ABA treatment in wheat. These findings provide knowledge for future studies of the functions of <i>TaGLK</i> TFs in wheat stress tolerance and development, which could have significant implications for enhancing wheat tolerance to various environmental stressors. |
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| series | Agronomy |
| spelling | doaj-art-83351851e1c44f30a5b8cd49f57b42d42024-12-27T14:04:54ZengMDPI AGAgronomy2073-43952024-12-011412307010.3390/agronomy14123070Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic StressJunhui Xiang0Pingu Liu1Daniel Bimpong2Jiayi Shen3Xusi Liu4Siting Wang5Yan Li6Youning Wang7Dongfang Ma8Ministry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaMinistry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaMinistry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaMinistry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaMinistry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaMinistry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaMinistry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaHubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, ChinaMinistry of Agriculture and Rural Affairs (MARA), Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, ChinaThe Golden2-like (GLK) transcription factors belong to the GARP family of transcription factors and play significant roles in plant growth, development, and responses to both abiotic and biotic stresses. This study employed bioinformatics and expression analyses to investigate the regulatory roles of wheat GLK proteins under various stress conditions, including abscisic acid (ABA) treatment, osmotic stress, and infection by <i>Fusarium graminearum</i>. The study identified 125 <i>TaGLK</i> proteins and revealed that <i>TaGLKs</i> play a significant role in wheat’s development and response to adverse environmental conditions. The results indicate that <i>TaGLKs</i> may serve as potential transcriptional regulators capable of integrating multiple cellular signals to coordinate various developmental and physiological processes. Evolutionary analysis classified the <i>TaGLK</i> proteins into six subgroups, which shared similar conserved domains and motifs. Protein–protein interaction network analysis revealed that <i>TaGLKs</i> are involved in photoreceptor activity, cell cycle progression, and protein regulation. Gene expression analysis of <i>TaGLKs</i> discovered that they play key functions in wheat development, as well as regulation of biotic and abiotic stress conditions. RT-qPCR analysis showed that <i>TaGLKs</i> regulate earlier and late effects of osmotic stress, <i>F. graminearum</i> infections, and ABA treatment in wheat. These findings provide knowledge for future studies of the functions of <i>TaGLK</i> TFs in wheat stress tolerance and development, which could have significant implications for enhancing wheat tolerance to various environmental stressors.https://www.mdpi.com/2073-4395/14/12/3070wheatGolden2-like gene familybiotic and abiotic stressexpression analysis |
| spellingShingle | Junhui Xiang Pingu Liu Daniel Bimpong Jiayi Shen Xusi Liu Siting Wang Yan Li Youning Wang Dongfang Ma Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic Stress Agronomy wheat Golden2-like gene family biotic and abiotic stress expression analysis |
| title | Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic Stress |
| title_full | Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic Stress |
| title_fullStr | Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic Stress |
| title_full_unstemmed | Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic Stress |
| title_short | Identification and Expression Analysis of Wheat Golden2-like (<i>TaGLK</i>) Gene in Response to Biotic and Abiotic Stress |
| title_sort | identification and expression analysis of wheat golden2 like i taglk i gene in response to biotic and abiotic stress |
| topic | wheat Golden2-like gene family biotic and abiotic stress expression analysis |
| url | https://www.mdpi.com/2073-4395/14/12/3070 |
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