Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe
Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky’s disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequen...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2019/1806842 |
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| author | Zsolt Csabai Dóra Tombácz Zoltán Deim Michael Snyder Zsolt Boldogkői |
| author_facet | Zsolt Csabai Dóra Tombácz Zoltán Deim Michael Snyder Zsolt Boldogkői |
| author_sort | Zsolt Csabai |
| collection | DOAJ |
| description | Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky’s disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent. |
| format | Article |
| id | doaj-art-83094a43713d450ca0f167e6209faa06 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-83094a43713d450ca0f167e6209faa062025-02-03T05:47:25ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932019-01-01201910.1155/2019/18068421806842Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast EuropeZsolt Csabai0Dóra Tombácz1Zoltán Deim2Michael Snyder3Zsolt Boldogkői4Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged H-6720, HungaryDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged H-6720, HungaryDepartment of Biotechnology, Faculty of Science and Informatics, University of Szeged, Középfasor 52., Szeged H-6726, HungaryDepartment of Genetics, School of Medicine, Stanford University, 300 Pasteur Dr., Stanford, CA 94305-5120, USADepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged H-6720, HungaryBackground. Pseudorabies virus (PRV) is the causative agent of Aujeszky’s disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.http://dx.doi.org/10.1155/2019/1806842 |
| spellingShingle | Zsolt Csabai Dóra Tombácz Zoltán Deim Michael Snyder Zsolt Boldogkői Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe |
| title_full | Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe |
| title_fullStr | Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe |
| title_full_unstemmed | Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe |
| title_short | Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe |
| title_sort | analysis of the complete genome sequence of a novel pseudorabies virus strain isolated in southeast europe |
| url | http://dx.doi.org/10.1155/2019/1806842 |
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