Protocol to identify the signaling pathways of quorum sensing system in Burkholderia cenocepacia
Summary: Here, we present a protocol for identifying the components of a quorum sensing signaling system in bacteria. We describe the steps for characterizing the novel response regulator and receptor of the cis-2-dodecenoic acid (BDSF) quorum sensing signaling system in Burkholderia cenocepacia. Th...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672400546X |
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| Summary: | Summary: Here, we present a protocol for identifying the components of a quorum sensing signaling system in bacteria. We describe the steps for characterizing the novel response regulator and receptor of the cis-2-dodecenoic acid (BDSF) quorum sensing signaling system in Burkholderia cenocepacia. The technical assays of this protocol include generating a random mutant library, chromatin immunoprecipitation sequencing (ChIP-seq), electrophoretic mobility transfer assay (EMSA), microscale thermophoresis (MST), and molecular simulation docking.For complete details on the use and execution of this protocol, please refer to Li et al.1 and Yang et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |