Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer
The detection and analysis of cancer cell exosomes with high sensitivity and precision are pivotal for the early diagnosis and treatment strategies of prostate cancer. To this end, a microfluidic chip, equipped with a cactus-like array substrate (CAS) based on surface-enhanced Raman spectroscopy (SE...
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MDPI AG
2024-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/12/579 |
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| author | Huakun Jia Weiyang Meng Rongke Gao Yeru Wang Changbiao Zhan Yiyue Yu Haojie Cong Liandong Yu |
| author_facet | Huakun Jia Weiyang Meng Rongke Gao Yeru Wang Changbiao Zhan Yiyue Yu Haojie Cong Liandong Yu |
| author_sort | Huakun Jia |
| collection | DOAJ |
| description | The detection and analysis of cancer cell exosomes with high sensitivity and precision are pivotal for the early diagnosis and treatment strategies of prostate cancer. To this end, a microfluidic chip, equipped with a cactus-like array substrate (CAS) based on surface-enhanced Raman spectroscopy (SERS) was designed and fabricated for the detection of exosome concentrations in Lymph Node Carcinoma of the Prostate (LNCaP). Double layers of polystyrene (PS) microspheres were self-assembled onto a polyethylene terephthalate (PET) film to form an ordered cactus-like nanoarray for detection and analysis. By combining EpCAM aptamer-labeled SERS nanoprobes and a CD63 aptamer-labeled CAS, a ‘sandwich’ structure was formed and applied to the microfluidic chips, further enhancing the Raman scattering signal of Raman reporter molecules. The results indicate that the integrated microfluidic sensor exhibits a good linear response within the detection concentration range of 10<sup>5</sup> particles μL<sup>−1</sup> to 1 particle μL<sup>−1</sup>. The detection limit of exosomes in cancer cells can reach 1 particle μL<sup>−1</sup>. Therefore, we believed that the CAS integrated microfluidic sensor offers a superior solution for the early diagnosis and therapeutic intervention of prostate cancer. |
| format | Article |
| id | doaj-art-82d986ff12144417ba3b4a5dd10d97a7 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-82d986ff12144417ba3b4a5dd10d97a72024-12-27T14:14:11ZengMDPI AGBiosensors2079-63742024-11-01141257910.3390/bios14120579Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate CancerHuakun Jia0Weiyang Meng1Rongke Gao2Yeru Wang3Changbiao Zhan4Yiyue Yu5Haojie Cong6Liandong Yu7State Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaState Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaState Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaState Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaState Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaState Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaState Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaState Key Laboratory of Chemical Safety, College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, ChinaThe detection and analysis of cancer cell exosomes with high sensitivity and precision are pivotal for the early diagnosis and treatment strategies of prostate cancer. To this end, a microfluidic chip, equipped with a cactus-like array substrate (CAS) based on surface-enhanced Raman spectroscopy (SERS) was designed and fabricated for the detection of exosome concentrations in Lymph Node Carcinoma of the Prostate (LNCaP). Double layers of polystyrene (PS) microspheres were self-assembled onto a polyethylene terephthalate (PET) film to form an ordered cactus-like nanoarray for detection and analysis. By combining EpCAM aptamer-labeled SERS nanoprobes and a CD63 aptamer-labeled CAS, a ‘sandwich’ structure was formed and applied to the microfluidic chips, further enhancing the Raman scattering signal of Raman reporter molecules. The results indicate that the integrated microfluidic sensor exhibits a good linear response within the detection concentration range of 10<sup>5</sup> particles μL<sup>−1</sup> to 1 particle μL<sup>−1</sup>. The detection limit of exosomes in cancer cells can reach 1 particle μL<sup>−1</sup>. Therefore, we believed that the CAS integrated microfluidic sensor offers a superior solution for the early diagnosis and therapeutic intervention of prostate cancer.https://www.mdpi.com/2079-6374/14/12/579SERSmicrofluidic chiphierarchical interfaceexosomeearly diagnosis of cancer |
| spellingShingle | Huakun Jia Weiyang Meng Rongke Gao Yeru Wang Changbiao Zhan Yiyue Yu Haojie Cong Liandong Yu Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer Biosensors SERS microfluidic chip hierarchical interface exosome early diagnosis of cancer |
| title | Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer |
| title_full | Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer |
| title_fullStr | Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer |
| title_full_unstemmed | Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer |
| title_short | Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer |
| title_sort | integrated sers microfluidic sensor based on nano micro hierarchical cactus like array substrates for the early diagnosis of prostate cancer |
| topic | SERS microfluidic chip hierarchical interface exosome early diagnosis of cancer |
| url | https://www.mdpi.com/2079-6374/14/12/579 |
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