Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice cap
Microbes play an essential role in nutrient turnover within Arctic environments, and their contribution to biogeochemical cycles can depend on several factors, including but not limited to cell viability. In this study, we employed the SYBR-PI dual cell stain to epifluorescence microscopy to enumera...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Arctic, Antarctic, and Alpine Research |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15230430.2024.2407711 |
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| author | Archana Dayal Andrew Hodson Marie Šabacká Alan Smalley |
| author_facet | Archana Dayal Andrew Hodson Marie Šabacká Alan Smalley |
| author_sort | Archana Dayal |
| collection | DOAJ |
| description | Microbes play an essential role in nutrient turnover within Arctic environments, and their contribution to biogeochemical cycles can depend on several factors, including but not limited to cell viability. In this study, we employed the SYBR-PI dual cell stain to epifluorescence microscopy to enumerate proportions of potentially viable and non-viable bacterial cell populations within a melting snowpack on an ice cap, Foxfonna in Svalbard. Non-viable cells dominated on Foxfonna (2.5 ± 0.36 × 107 cells m−2) during the June to early July period, when biological production was usually at its peak. Furthermore, non-viable cells also dominated the total cell abundance within superimposed ice (223 ± 242 cells mL−1) and glacial ice (695 ± 717 cells mL−1) beneath the snow. We propose that the rapid, early loss of cell viability was caused by a number of abiotic and biotic factors. Hence, necromass (dead cell residue) contributed to the export of organic matter to downstream ecosystems. |
| format | Article |
| id | doaj-art-82a8ec05694b4732bc8d51bceedfd21c |
| institution | Kabale University |
| issn | 1523-0430 1938-4246 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Arctic, Antarctic, and Alpine Research |
| spelling | doaj-art-82a8ec05694b4732bc8d51bceedfd21c2025-01-13T14:40:40ZengTaylor & Francis GroupArctic, Antarctic, and Alpine Research1523-04301938-42462024-12-0156110.1080/15230430.2024.2407711Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice capArchana Dayal0Andrew Hodson1Marie Šabacká2Alan Smalley3Department of Life Sciences, Aberystwyth University, Aberystwyth, UKArctic Geology Department, University Centre in Svalbard, Longyearbyen, NorwayCentre for Polar Ecology, University of South Bohemia, České Budějovice, Czech RepublicDepartment of Geography, University of Sheffield, Sheffield, UKMicrobes play an essential role in nutrient turnover within Arctic environments, and their contribution to biogeochemical cycles can depend on several factors, including but not limited to cell viability. In this study, we employed the SYBR-PI dual cell stain to epifluorescence microscopy to enumerate proportions of potentially viable and non-viable bacterial cell populations within a melting snowpack on an ice cap, Foxfonna in Svalbard. Non-viable cells dominated on Foxfonna (2.5 ± 0.36 × 107 cells m−2) during the June to early July period, when biological production was usually at its peak. Furthermore, non-viable cells also dominated the total cell abundance within superimposed ice (223 ± 242 cells mL−1) and glacial ice (695 ± 717 cells mL−1) beneath the snow. We propose that the rapid, early loss of cell viability was caused by a number of abiotic and biotic factors. Hence, necromass (dead cell residue) contributed to the export of organic matter to downstream ecosystems.https://www.tandfonline.com/doi/10.1080/15230430.2024.2407711Snowpackviable cellsnon-viable cellsSvalbardHigh Arctic |
| spellingShingle | Archana Dayal Andrew Hodson Marie Šabacká Alan Smalley Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice cap Arctic, Antarctic, and Alpine Research Snowpack viable cells non-viable cells Svalbard High Arctic |
| title | Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice cap |
| title_full | Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice cap |
| title_fullStr | Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice cap |
| title_full_unstemmed | Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice cap |
| title_short | Seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a High Arctic ice cap |
| title_sort | seasonal changes in the viability and abundance of bacterial cells in the snowpack ecosystem of a high arctic ice cap |
| topic | Snowpack viable cells non-viable cells Svalbard High Arctic |
| url | https://www.tandfonline.com/doi/10.1080/15230430.2024.2407711 |
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