Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship
<b>Background/Objectives:</b> Beta-lactam antibiotics, derived from penicillin, are the most used class of antimicrobials used for treating bacterial infections. Over the years, microorganisms have developed resistance mechanisms capable of preventing the effect of these drugs. This cond...
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MDPI AG
2024-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/12/1157 |
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| author | Vinicius Pereira Anjos Caroline Guimarães Pançardes da Silva Marangoni Rafael Nadas Thiago Neves Machado Damaris Krul Luiza Souza Rodrigues Libera Maria Dalla-Costa Wido Herwig Schreiner Denise Maria Zezell Arandi Ginane Bezerra Rafael Eleodoro de Góes |
| author_facet | Vinicius Pereira Anjos Caroline Guimarães Pançardes da Silva Marangoni Rafael Nadas Thiago Neves Machado Damaris Krul Luiza Souza Rodrigues Libera Maria Dalla-Costa Wido Herwig Schreiner Denise Maria Zezell Arandi Ginane Bezerra Rafael Eleodoro de Góes |
| author_sort | Vinicius Pereira Anjos |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Beta-lactam antibiotics, derived from penicillin, are the most used class of antimicrobials used for treating bacterial infections. Over the years, microorganisms have developed resistance mechanisms capable of preventing the effect of these drugs. This condition has been a significant public health concern for the 21st century, especially after predictions that antimicrobial resistance could lead to 10 million deaths annually by 2050. The challenge of developing new antimicrobials brings with it the need to ensure the efficacy of existing ones, hence the importance of developing fast and low-cost monitoring techniques. <b>Methods:</b> In this study, we present an alternative based on nanophotonics using Surface-Enhanced Raman Spectroscopy (SERS) mediated by nanoparticles for the detection of antimicrobials, with emphasis on some beta-lactam antibiotics commonly prescribed in cases of critically ill patients. It is a sensitive and accurate technique for drug monitoring, allowing for rapid and specific detection of its molecular signatures. This approach is crucial to address the challenge of antimicrobial resistance and ensure the therapeutic efficacy of existing treatments. <b>Results:</b> Our experiments demonstrate the possibility of identifying spectra with characteristic vibrations (fingerprints) of these antimicrobials via SERS. <b>Conclusions:</b> Our results point to new strategies for molecular monitoring of drugs by optical techniques using unconventional nanoparticles. |
| format | Article |
| id | doaj-art-82a2033cfc8f42f88a0fe51badc18cda |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-82a2033cfc8f42f88a0fe51badc18cda2024-12-27T14:06:12ZengMDPI AGAntibiotics2079-63822024-12-011312115710.3390/antibiotics13121157Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial StewardshipVinicius Pereira Anjos0Caroline Guimarães Pançardes da Silva Marangoni1Rafael Nadas2Thiago Neves Machado3Damaris Krul4Luiza Souza Rodrigues5Libera Maria Dalla-Costa6Wido Herwig Schreiner7Denise Maria Zezell8Arandi Ginane Bezerra9Rafael Eleodoro de Góes10Laboratório de Biofotônica, Centro de Lasers e Aplicações, Instituto de Pesquisas Energéticas e Nucleares, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório Fotonanobio, Programa de Pós-Graduação em Física e Astronomia, Universidade Tecnológica Federal do Paraná, Curitiba 82590-300, BrazilLaboratório Fotonanobio, Programa de Pós-Graduação em Física e Astronomia, Universidade Tecnológica Federal do Paraná, Curitiba 82590-300, BrazilLaboratório Fotonanobio, Programa de Pós-Graduação em Física e Astronomia, Universidade Tecnológica Federal do Paraná, Curitiba 82590-300, BrazilInstituto de Pesquisas Pelé Pequeno Príncipe, Faculdades Pequeno Príncipe, Curitiba 80250-060, BrazilInstituto de Pesquisas Pelé Pequeno Príncipe, Faculdades Pequeno Príncipe, Curitiba 80250-060, BrazilInstituto de Pesquisas Pelé Pequeno Príncipe, Faculdades Pequeno Príncipe, Curitiba 80250-060, BrazilLaboratório Fotonanobio, Programa de Pós-Graduação em Física e Astronomia, Universidade Tecnológica Federal do Paraná, Curitiba 82590-300, BrazilLaboratório de Biofotônica, Centro de Lasers e Aplicações, Instituto de Pesquisas Energéticas e Nucleares, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório Fotonanobio, Programa de Pós-Graduação em Física e Astronomia, Universidade Tecnológica Federal do Paraná, Curitiba 82590-300, BrazilLaboratório Fotonanobio, Programa de Pós-Graduação em Física e Astronomia, Universidade Tecnológica Federal do Paraná, Curitiba 82590-300, Brazil<b>Background/Objectives:</b> Beta-lactam antibiotics, derived from penicillin, are the most used class of antimicrobials used for treating bacterial infections. Over the years, microorganisms have developed resistance mechanisms capable of preventing the effect of these drugs. This condition has been a significant public health concern for the 21st century, especially after predictions that antimicrobial resistance could lead to 10 million deaths annually by 2050. The challenge of developing new antimicrobials brings with it the need to ensure the efficacy of existing ones, hence the importance of developing fast and low-cost monitoring techniques. <b>Methods:</b> In this study, we present an alternative based on nanophotonics using Surface-Enhanced Raman Spectroscopy (SERS) mediated by nanoparticles for the detection of antimicrobials, with emphasis on some beta-lactam antibiotics commonly prescribed in cases of critically ill patients. It is a sensitive and accurate technique for drug monitoring, allowing for rapid and specific detection of its molecular signatures. This approach is crucial to address the challenge of antimicrobial resistance and ensure the therapeutic efficacy of existing treatments. <b>Results:</b> Our experiments demonstrate the possibility of identifying spectra with characteristic vibrations (fingerprints) of these antimicrobials via SERS. <b>Conclusions:</b> Our results point to new strategies for molecular monitoring of drugs by optical techniques using unconventional nanoparticles.https://www.mdpi.com/2079-6382/13/12/1157beta-lactamdrug monitoringantibioticsRamanSERSnanoparticles |
| spellingShingle | Vinicius Pereira Anjos Caroline Guimarães Pançardes da Silva Marangoni Rafael Nadas Thiago Neves Machado Damaris Krul Luiza Souza Rodrigues Libera Maria Dalla-Costa Wido Herwig Schreiner Denise Maria Zezell Arandi Ginane Bezerra Rafael Eleodoro de Góes Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship Antibiotics beta-lactam drug monitoring antibiotics Raman SERS nanoparticles |
| title | Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship |
| title_full | Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship |
| title_fullStr | Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship |
| title_full_unstemmed | Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship |
| title_short | Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship |
| title_sort | identifying the molecular fingerprint of beta lactams via raman sers spectroscopy using unconventional nanoparticles for antimicrobial stewardship |
| topic | beta-lactam drug monitoring antibiotics Raman SERS nanoparticles |
| url | https://www.mdpi.com/2079-6382/13/12/1157 |
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