The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>)
This study compared the quality of northern pike eggs collected using traditional methods (hand stripping) and pneumatic methods (air stripping). The effects of different activation solutions (0.4% NaCl, 0.8% NaCl, and hatchery water) on egg fertilization under controlled conditions were also invest...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/1/8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study compared the quality of northern pike eggs collected using traditional methods (hand stripping) and pneumatic methods (air stripping). The effects of different activation solutions (0.4% NaCl, 0.8% NaCl, and hatchery water) on egg fertilization under controlled conditions were also investigated. After egg collection, the Pseudo-Gonado-Somatic Index (PGSI) was measured; the PGSI values in the samples obtained using the pneumatic method (13.8 ± 3.9%) were lower, but did not differ statistically from those obtained by hand stripping (16.5 ± 5.4%). The 0.4% NaCl solution proved to be the most effective for sperm activation, as assessed by the Computer-Assisted Sperm Analysis (CASA) system, compared to the 0.8% NaCl solution. The pneumatic method achieved a higher egg collection efficiency (93.7% occlusion) than the traditional method, with significant differences observed in groups activated with water. The average hatching percentage of larvae was 89.5% in groups using the pneumatic method, compared to 71.2% in the traditional groups, highlighting the advantages of this modern approach. The application of the pneumatic method and 0.4% NaCl for the artificial fertilization of northern pike resulted in higher fertilization and hatching rates compared to other techniques, making this method a promising option for the artificial reproduction of other fish species. |
|---|---|
| ISSN: | 2076-2615 |